Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 32, Pages 4140-4146Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2009.09.041
Keywords
HMMA(VMA); HVA; 5-HIAA; Neuroblastoma; Carcinoid syndrome; Pheochromocytoma
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A validated, high pressure liquid chromatographic (HPLC) method for simultaneous quantitation of urinary catecholic acids 4-hydroxy-3-methoxymandelic acid (HMMA) (vanylmandelic acid) (VMA), 4-hydroxy-3-methoxyphenylacetic acid (HVA) and 5-hydroxyindole-3-acetic acid (5-HIAA) was developed. Sample preparation involved liquid-liquid extraction of acidified urine, containing iso-HMMA (IS) as internal standard, with ether, evaporation of the organic extract, followed by reconstitution of the residue in phosphate buffer at pH 3.3. After reversed-phase HPLC at 35 degrees C and separation on a Licrospher 125 mm x 4 mm C(18) column (5 mu m particle size) with phosphate buffer (pH 3.5)-acetone (950:50, v/v) as eluent, quantitation is achieved by electrochemical detection using coulometric detection at a potential of +350 mV. The method was successfully applied to routine diagnosis of neuroblastoma. carcinoid syndrome and pheochromocytoma. (C) 2009 Elsevier B.V. All rights reserved.
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