Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 13, Pages 1388-1393Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2009.02.005
Keywords
1,3-Butadiene; DHBMA; Occupational exposure; Biological monitoring; HPLC-MS/MS; Method validation; Polynomial regression
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1,3-Butadiene is used in the production of synthetic rubber and is also a widespread environmental pollutant, produced by car exhaust, heating and cigarette smoke. According to IARC it is probably carcinogenic to humans. A method was developed and validated for the quantification in human urine of 1,2-dihydroxybutyl mercapturic acid, a butacliene metabolite for which the American Conference of Governmental Hygienists suggests a biological exposure index of 2500 mu g/L. Solid phase extraction was used for analyte extraction and HPLC-MS/MS for detection. The calibration range from 20 to 2500 mu g/L required the use of polynomial calibration curves, and the performance of the analytical method was tested according to an international validation guideline. Accuracy was never less than 85%, precision always higher than 15% and the LOD 3.6 mu g/L The method was applied to 33 non-smokers, non-occupationally exposed to butadiene, and gave urinary concentrations between 16 and 599 mu g/L. (C) 2009 Elsevier B.V. All rights reserved.
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