Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 877, Issue 32, Pages 4159-4163Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2009.11.001
Keywords
UPLC; HPLC; Silymarin; Gradient analysis
Funding
- Natural Science Foundation of China [20776009]
- Program for New Century Excellent Talents [NCET-05-0117]
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A novel rapid chromatographic method based on utilization of UPLC column was developed for the analysis of eight active compounds in silymarin. The analysis was performed on a Waters Acquity UPLC system with an Acquity UPLCBEH C18 column (5 mm x 2.1 mm I.D., 1.7 mu m) and a gradient elution of methanol and water containing 0.01% formic acid with a run time of 9 min, in which the retention time of the last analyte was 5.8 min. And all eight active compounds achieved complete separation. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency and sensitivity. The results indicated that the type of column. the type of mobile phase and the modified addition were significant to the separation of isomeric compounds in herb extracts. (C) 2009 Elsevier B.V. All rights reserved.
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