Article
Biotechnology & Applied Microbiology
Eduardo Gushiken, Milagros Quintana, Luz Agueda Perez, Patricia Herrera-Velit, Elizabeth R. Balmayor, Martijn van Griensven, Jose R. Espinoza
Summary: This study successfully isolated ASCs using anti-CD105 VHH conjugated magnetic beads, which showed high selectivity and recovery rate. This method may provide a new strategy for MSCs isolation.
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Cell Biology
Qiong Li, Huifang Hou, Meng Li, Xia Yu, Hongbo Zuo, Jianhui Gao, Min Zhang, Zongjin Li, Zhikun Guo
Summary: The research demonstrates the effective role of CD73(+) AD-MSCs in treating myocardial infarction by promoting angiogenesis for accelerating cardiac recovery. This subgroup shows differences in the expression of factors like VEGF compared to other subgroups, suggesting its potential superiority in myocardial repair.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Kristeen Ye Wen Teo, Shipin Zhang, Jia Tong Loh, Ruenn Chai Lai, Hwee Weng Dennis Hey, Kong-Peng Lam, Sai Kiang Lim, Wei Seong Toh
Summary: MSC exosomes promote the polarization of M2-like macrophages through activation of the toll-like receptor signaling pathway and CD73-mediated adenosine production. The production of adenosine activates AKT/ERK-dependent signaling pathways via binding to adenosine receptors A(2A) and A(2B). These findings highlight the critical role of CD73 in mediating M2-like macrophage polarization by MSC exosomes, and have implications for predicting the immunomodulatory potency of MSC exosome preparations.
Article
Biotechnology & Applied Microbiology
Xiao Zhai, Kai Chen, Huan Yang, Bo Li, Tianjunke Zhou, Haojue Wang, Huipeng Zhou, Shaofeng Chen, Xiaoyi Zhou, Xiaozhao Wei, Yushu Bai, Ming Li
Summary: CD73+ hucMSC-EVs ameliorated inflammation after spinal cord injury by reducing extracellular ATP, promoting A2bR/cAMP/PKA pathway and M2/M1 polarization. They may be promising nano drugs for clinical application in SCI therapy.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lu Cao, Kim R. Bridle, Ritu Shrestha, Prashanth Prithviraj, Darrell H. G. Crawford, Aparna Jayachandran
Summary: Gallbladder cancer is a highly aggressive form of biliary tract cancer, and current clinical trials are examining the effectiveness of immune checkpoint inhibitors as monotherapy. However, only select patients respond to these treatments. This study investigates the molecular mechanisms that contribute to resistance to immune checkpoint blockade and identifies epithelial-to-mesenchymal transition and cancer stem cells as key factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Tao Chen, Ting Xu, Mingye Cheng, Hao Fang, Xianjuan Shen, Zhiyuan Tang, Jianmei Zhao
Summary: The study evaluated the expression levels of CD31(+) CD54(+) and CD31(+) CD105(+) endothelial microparticles in Kawasaki disease patients before and after treatment, finding that hucMSCs can inhibit endothelial inflammation in KD patients and show similar effects in mouse experiments.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Chemistry, Medicinal
Bruna C. Galgaro, Liziane R. Beckenkamp, Martha van den M. Nunnenkamp, Vitoria G. Korb, Liliana I. S. Naasani, Katarzyna Roszek, Marcia R. Wink
Summary: Mesenchymal stem cells (MSCs) have various important functions in tissue homeostasis, damage repair, and immune modulation, and their therapeutic potential is closely related to the CD73/adenosine-mediated signaling pathway. Further research is needed to fully understand the role of CD73 and adenosine in the therapeutic functions of MSCs.
MEDICINAL RESEARCH REVIEWS
(2021)
Review
Polymer Science
Aiah A. El-Rashidy, Sara El Moshy, Israa Ahmed Radwan, Dina Rady, Marwa M. S. Abbass, Christof E. Doerfer, Karim M. Fawzy El-Sayed
Summary: The stiffness of the extracellular matrix plays a regulatory role in directing the osteogenic differentiation of MSCs, with MSCs sensing and responding to their ECM to undergo differentiation. Customizing scaffold materials to mimic cells' natural environment is crucial in the tissue engineering process.
Article
Biology
Samira Diar-Bakirly, Tarek El-Bialy
Summary: This study investigated the differentiation potential of GMSCs based on CD146 expression, indicating that solely relying on CD146 for identifying gingival stem cells may not be adequate for translational research on novel therapeutic methods for treating oral disease.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Ophthalmology
Jilu Jaffet, Aparna Mohanty, Induvahi Veernala, Swati Singh, Mohammad Javed Ali, Sayan Basu, Geeta K. Vemuganti, Vivek Singh
Summary: This study provides the first evidence of the presence of mesenchymal stem cell (MSC) population within human lacrimal glands (LGs), suggesting their potential role in maintaining a healthy microenvironment within normal LGs and repairing diseased LGs.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Luminita Labusca, Dumitru-Daniel Herea, Anca Emanuela Minuti, Cristina Stavila, Camelia Danceanu, Petru Plamadeala, Horia Chiriac, Nicoleta Lupu
Summary: Iron oxide based magnetic nanoparticles (MNP) have versatile applications in biology and medicine. This study compared the chondrogenic conversion of ADSC-MNP and WJMSC under different conditions, finding that ADSC-MNP displayed superior proliferation and chondrogenic potential, with MF exposure further enhancing chondrogenesis in ADSC-MNP. Loading ADSC with MNP may be a promising approach for cartilage engineering in the future.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Gustavo A. Higuera, Tiago Ramos, Antonio Gloria, Luigi Ambrosio, Andrea Di Luca, Nicholas Pechkov, Joost R. de Wijn, Clemens A. van Blitterswijk, Lorenzo Moroni
Summary: Additively manufactured scaffolds have custom-shaped structures with completely interconnected pore networks, and the surface properties of scaffolds greatly impact the success of engineered tissue structures. By combining microspheres with additive manufacturing technologies, 3D scaffolds with adjustable surface roughness can enhance cell adhesion and metabolic activity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Cell Biology
Aisha Naeem, Nikita Gupta, Usra Naeem, Muhammad Jawad Khan, Mohamed A. Elrayess, Wanxing Cui, Chris Albanese
Summary: The successful translation of MSCs from bench to bedside relies on their regenerative capabilities and immunomodulatory potential. Adult tissue-derived MSCs face challenges in sourcing and characterization, while alternative sources, such as amniotic membrane, offer advantages for clinical applications with optimized culture methods.
Article
Polymer Science
Mia Arifka, Gofarana Wilar, Khaled M. Elamin, Nasrul Wathoni
Summary: The polymeric hydrogels have been shown to have great potential as MSC secretome delivery systems in biomedical applications such as tissue engineering, drug delivery, and cancer therapy. They provide a friendly environment, control the distribution of bioactive compounds in tissues, and allow for controlled and sustained release.
Article
Veterinary Sciences
Min-Soo Seo, Kyung-Ku Kang, Se-Kyung Oh, Soo-Eun Sung, Kil-Soo Kim, Young-Sam Kwon, Sungho Yun
Summary: Wharton's jelly is a known source of mesenchymal stem cells in various species, but there have been no reports on cats. This study isolated and characterized mesenchymal stem cells from the Wharton's jelly of cats, demonstrating their differentiation potential and therapeutic applications in feline medicine.
VETERINARY SCIENCES
(2021)
Article
Cell & Tissue Engineering
Umran Aydemir Sezer, Kevser Ozturk Yavuz, Gizem Ors, Sadik Bay, Basak Aru Aru, Oguz Sogut, Tuba Akgul Caglar, Mehmet Recep Bozkurt, Esra Cagavi Cagavi, Gulderen Yanikkaya Demirel Yanikkaya Demirel, Serdar Sezer, Huseyin Karaca
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Article
Immunology
Claude Lambert, Gulderen Yanikkaya Demirel, Thomas Keller, Frank Preijers, Katherina Psarra, Matthias Schiemann, Mustafa Ozcurumez, Ulrich Sack
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Obstetrics & Gynecology
Sebnem Alanya Tosun, Enis Ozkaya, Basak Aru, Gulderen Yanikkaya Demirel, Ebru Cogendez, Mehmet Sipahi
Summary: The study demonstrates that LH supplementation during COS for PORs improves GC viability and reduces early/late apoptosis rates, leading to a higher number of MII oocytes. However, this does not result in a significant difference in clinical pregnancy rates. Further multi-centre research is needed to validate these findings.
JOURNAL OF OBSTETRICS AND GYNAECOLOGY
(2022)
Review
Oncology
Basak Aru, Mojdeh Soltani, Cemil Pehlivanoglu, Ege Guerlue, Mazdak Ganjalikhani-Hakemi, Guelderen Yanikkaya Demirel
Summary: Immune checkpoint inhibitors have limited clinical benefits in hematological malignancies, and may cause side effects and resistance mechanisms. Laboratory biomarkers are needed to predict efficacy and manage side effects, but there is a lack of consensus on guidelines and parameters.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Murat Erdogan, Basak Aru, Umut Can Taygun, Ceren Simsek, Erdem Yesilada, Gulderen Yanikkaya-Demirel, Hasan Kirmizibekmez
Summary: This study aimed to isolate cytotoxic compounds from V. alliariifolia, determine their mechanisms of action, and investigate their anticancer potential. Fourteen secondary metabolites were isolated, and compounds 2, 4-6, and 8 showed potent cytotoxicity against cancer cells. These compounds exerted anticancer effects through different cell death mechanisms.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Plant Sciences
Murat Erdogan, Basak Aru, Ceren Ozturk, Olcay Esin Ozdemir, Erdem Yesilada, Gulderen Yanikkaya-Demirel, Hasan Kirmizibekmez
Summary: This study conducted phytochemical and bioactivity research on Valeriana speluncaria, an endemic species to Turkey, and isolated two unreported neolignans and seven known compounds through cytotoxic activity-guided fractionation method.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Pharmacology & Pharmacy
Tugce Onel, Cihan S. Erdogan, Basak Aru, Ecem Yildirim, Gulderen Yanikkaya Demirel, Aylin Yaba
Summary: mTOR pathway is an important kinase that regulates cell cycle, protein synthesis, and energy metabolism. In this study, rapamycin treatment effectively inhibited mTOR and P70S6K activity in TCam-2 cells, leading to suppressed cell proliferation and induced apoptosis. These findings contribute to the development of new treatment approaches for seminoma and preservation of male fertility.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Murat Erdogan, Basak Aru, Seref Tayga Yilmaz, Erdem Yesilada, Gulderen Yanikkaya-Demirel, Hasan Kirmizibekmez
Summary: Cytotoxic activity-guided isolation studies identified 12 secondary metabolites from Valeriana sisymbriifolia, including two undescribed iridoids and two unreported sesquiterpene lactones. Extensive analysis was performed to establish the chemical structures of the isolates. In vitro cytotoxicity evaluations showed that certain compounds exhibited promising activity against cancer cell lines, with valtrate and 7-homovaltrate being particularly potent against multiple cell lines.
Article
Medicine, General & Internal
Basak Aru, Tuba Akdeniz, Husniye Dagdeviren, Gizem Gurel, Gulderen Yanikkaya Demirel
Summary: This study aimed to investigate the effects of testosterone propionate on the proliferation and viability of human bone marrow-derived mesenchymal stem cells. It was found that testosterone propionate promoted cell proliferation and maintained viability, as well as increasing cytotoxicity.
BALKAN MEDICAL JOURNAL
(2023)
Article
Medicine, Research & Experimental
Duygu Misirli, Ozlem Bingol Ozakpinar, Turgut Sekerler, Basak Aru, Gulderen Yanikkaya Demirel, Servet Tunoglu, Derya Ozsavci
Summary: The study aimed to observe the effects of exogenous SPARC on colon cancer cell line HT-29, investigate the possible receptors stabilin-1 and integrin alpha v beta 3, and determine the receptor numbers. The appropriate doses of SPARC and its inhibitor cilengitide were determined, caspase 3 expressions were measured, and gene expression levels and receptor numbers were detected. The results showed that exogenous SPARC reduced proliferation and induced apoptosis in colon cancer cells, with integrin alpha v beta 3 considered as a possible mediating receptor for SPARC. Quantification of surface receptors per cell can be a marker for anticancer treatments.
CLINICAL AND EXPERIMENTAL HEALTH SCIENCES
(2023)
Review
Immunology
Basak Aru, Gizem Gurel, Gulderen Yanikkaya Demirel
Summary: Mesenchymal stem cells, a subgroup of heterogeneous cells, have multipotent differentiation capabilities and can modulate immune responses. They have become widely used in regenerative medicine due to their accessibility and ability to be propagated in vitro. However, their heterogeneity poses challenges in clinical practice.
TURKISH JOURNAL OF IMMUNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Basak Aru, Gizem Gumusgoz Celik, Kevser Harmandar, Belgin Sahin, Ayse Gul Gurek, Devrim Atilla, Gulderen Yanikkaya Demirel
Summary: Long-term use of NSAIDs has been proven to reduce the risk of colorectal cancer development. This study evaluated the anti-cancer activity and molecular mechanisms of two different NSAIDs substituted silicon phthalocyanine derivatives in various colorectal cancer cell lines.
ACS APPLIED BIO MATERIALS
(2022)
Meeting Abstract
Plant Sciences
M. Erdogan, B. Aru, S. T. Yilmaz, E. Yesilada, Yanikkaya G. Demirel, H. Kirmizibekmez
Article
Chemistry, Multidisciplinary
Basak Aru, Aysel Gunay, Gulderen Yanikkaya Demirel, Ayse Gul Gurek, Devrim Atilla
Summary: This study synthesized and characterized different Pc derivatives for anti-cancer efficacy, showing varied effects on breast cancer and endothelial cell lines. In particular, InPc derivatives exhibited enhanced effects on the mitochondrial network, while non-peripheral substitutions led to decreased anti-cancer activity. Further research is needed to determine ideal Pc candidates for anti-cancer PDT treatments.
Review
Immunology
Zeynep Akbulut, Gulderen Yanikkaya Demirel
Summary: KREC is a molecular structure that occurs in response to B lymphocytes, protecting the immune system against various antigens and serving as an indicator of early immune response, especially in immunodeficiency patients. It can be measured through molecular analysis to show B cell output from the bone marrow and has been increasingly studied in clinical settings. The mechanism of KREC occurrence, importance of KREC measurement, and its role in disease treatment are the main focus of this review.
TURKISH JOURNAL OF IMMUNOLOGY
(2021)