Article
Biochemical Research Methods
Mohamed A. Abdelaziz, Fotouh R. Mansour, Neil D. Danielson
Summary: A hydrophobic gadolinium-based magnetic ionic liquid was studied as an extraction solvent in DLLME for the first time, showing higher extraction efficiency than iron or manganese analogues, leading to improved sensitivity and reproducibility of the method, along with a reduced analysis time.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiangdong Ding, Chao Liu, Wei Yu, Zhongling Liu
Summary: A green, efficient and easy method combining magnetic ionic liquid-based liquid-liquid microextraction (MIL-based LLME) with ultraperformance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC-QqQ/ MS2) was developed for simultaneous determination of neurotransmitters (NTs) in biosamples. The selected magnetic ionic liquid [P6,6,6,14]2[CoCl4] showed advantages of visual recognition, paramagnetic behavior and higher extraction efficiency. The proposed method achieved facile magnetic separation of MIL containing analytes from matrix and successfully extracted and determined 20 NTs in human cerebrospinal fluid and plasma samples. The excellent analytical performance suggests the broad potential of this method for clinical diagnosis and therapy of neurological diseases.
Article
Food Science & Technology
Ainhoa Oller-Ruiz, Natalia Campillo, Manuel Hernandez-Cordoba, Javier Gilabert, Pilar Vinas
Summary: The study proposed using DLLME for preconcentration of lipophilic marine toxins in seawater samples. By optimizing the parameters and validating the method through recovery studies, the analysis showed that the water samples were free of the studied toxins.
Article
Chemistry, Multidisciplinary
Muzaffar Iqbal, Essam Ezzeldin, Nazrul Haq, Prawez Alam
Summary: A novel UPLC-MS/MS assay utilizing dispersive liquid-liquid microextraction and ultrasound-assisted reverse extraction has been developed for determination of multiclass pharmaceuticals in water samples. The method showed specificity and reliability for trace analysis of target analytes in waste water samples.
Article
Chemistry, Analytical
Lingling Wang, Xiaoxia Chen, Xueying Han, Baozhao Ju
Summary: A simple, green, rapid, and efficient magnet ionic liquid dispersive liquid-liquid microextraction method coupled with high-performance liquid chromatography was developed and used for the determination of three trace pyrethroids in water and food samples.
ANALYTICAL LETTERS
(2023)
Article
Chemistry, Applied
Jilong Li, Fengze Wu, Yupu Zhang, Ji Feng, Xiujuan Wang, Yi Yang, Zhibing Wang, Hanqi Zhang
Summary: A novel supramolecular solvent based on surface-active ionic liquid was prepared and successfully used for the extraction of four triazine herbicides in tea samples. The supramolecular solvent showed advantages of simple preparation process, low viscosity, and good dispersibility.
Article
Chemistry, Analytical
Maria Consolacion Rodriguez-Palazon, Natalia Arroyo-Manzanares, Pilar Vinas, Natalia Campillo
Summary: This paper presents a new analytical method using DLLME and UHPLC-HRMS for the determination of capsaicin in human urine. The method showed low detection limits and good repeatability, and was successfully applied to the analysis of urine from patients treated with capsaicin-based drugs.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemical Research Methods
Lorenza Schettino, Alejandro Garcia-Juan, Laura Fernandez-Lozano, Juan L. Benede, Alberto Chisvert
Summary: This study presents an analytical method for the determination of residual acrylamide in cosmetic products. The method involves vortex-assisted reversed-phase dispersive liquid-liquid microextraction (VA-RP-DLLME) using water as extraction solvent, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for detection. The method shows good analytical features, including high linearity, low limits of detection and quantification, and good repeatability. It is effective, simple, and fast, and can easily determine trace levels of acrylamide in cosmetic products, making it useful for quality control purposes.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Mahdi Bamorowat, Mohammad Reza Afshar Mogaddam, Mir Ali Farajzadeh
Summary: An effervescent tablet containing ionic liquids was developed and used in ultrasonic-assisted dispersive liquid-liquid microextraction for the extraction and preconcentration of benzoylurea insecticides in fruit juice and vegetable samples. The method achieved high sensitivity and simplicity.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Mahboob Nemati, Mir Ali Farajzadeh, Nail Altunay, Mustafa Tuzen, Sava Kaya, Mikhail M. Maslov, Mohammad Reza Afshar Mogaddam
Summary: In this study, a combination of dispersive solid phase extraction and ionic liquid-based dispersive liquid-liquid microextraction was used to extract aromatic amines from leather industries wastewater. The analytes were adsorbed by N and S doped carbon and then concentrated through microextraction. High performance liquid chromatography-ultraviolet detector was used for determination. The method was validated and showed good performance in terms of limits of detection and quantification, precision, enrichment factor, and extraction recovery. The approach was successfully applied to determine the analytes in wastewater samples from leather manufacturers.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Biochemical Research Methods
Marcio David Bocelli, Deyber Arley Vargas Medina, Julie Paulin Garcia Rodriguez, Fernando Mauro Lancas, Alvaro Jose Santos-Neto
Summary: Dynamic single-drop microextraction (SDME) was automated using an Arduino-based Cartesian robot and combined with liquid chromatography-tandem mass spectrometry for the determination of parabens in wastewater samples. The robot-assisted technique facilitated the control of dynamic SDME, making the process more feasible, robust, and reliable. This setup proved to be a competitive strategy for the automated extraction of organic pollutants from water samples.
Article
Chemistry, Analytical
Arshia Alizadeh Panahi, Afshin Javadi, Mohammad Reza Afshar Mogaddam
Summary: A novel method combining microwave-assisted extraction with magnetic ionic liquid-based dispersive liquid-liquid microextraction was developed for the extraction of chloramine-T from fish samples. This method achieved high extraction recovery, low limits of detection and quantification, good repeatability, and a wide linear range. The suggested method was successfully applied to analyze various fish samples marketed in Tabriz city, Iran.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Reyla A. S. Dias, Eliane R. Sousa, Gilmar S. Silva, Lanna K. Silva, Arlan S. Freitas, Diana L. D. Lima, Erika M. L. Sousa
Summary: This study developed an HPLC-FLD methodology for the separation of fluoroquinolones and optimized the DLLME process for clean-up and preconcentration of enrofloxacin in water samples. The method showed successful results in samples with low salinity content.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Valfredo Azevedo Lemos, Jeferson Alves Barreto, Luana Bastos Santos, Rosivan dos Santos de Assis, Cleber Galvao Novaes, Ricardo J. Cassella
Summary: Dispersive liquid-liquid microextraction (DLLME) is widely used for the separation and preconcentration of various chemical species, with some systems using a syringe as an extraction environment. This review discusses methods utilizing this approach, critical aspects like dispersion promotion, analytical characteristics, and the pros and cons of the methods, as well as future trends in in-syringe microextraction systems.
Article
Multidisciplinary Sciences
Ahmed Abdi Hassan, Abdulkadir Tanimu, Khalid Alhooshani
Summary: This study successfully utilized magnetic ionic liquid for the simultaneous preconcentration and quantification of 3 different categories of pharmaceutical products in wastewater and human urine samples. The method showed satisfactory results in terms of feasibility and accuracy.
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING
(2022)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)