Article
Food Science & Technology
Marta Leite, Andreia Freitas, Jorge Barbosa, Fernando Ramos
Summary: Mycotoxins, produced by fungi, can contaminate agricultural fields and food products, posing a threat to public health through animal exposure and subsequent milk contamination. Aflatoxin M1 is currently the only mycotoxin regulated in milk by the EU, but other mycotoxins can also be carried over. Therefore, it is important to develop precise and robust analytical methods to determine the occurrence of multiple mycotoxins in dairy products.
Article
Chemistry, Applied
Jesus M. Gonzalez-Jartin, Ines Rodriguez-Canas, Amparo Alfonso, Maria J. Sainz, Mercedes R. Vieytes, Ana Gomes, Isabel Ramos, Luis M. Botana
Summary: A simple method for quantifying 40 mycotoxins in milk was developed using QuEChERS extraction and UHPLC-MS/MS detection, achieving low LOQs, high recoveries, and acceptable performance characteristics in line with European regulations. The method successfully quantified mycotoxins in raw milk, highlighting the high occurrence of beauvericin and enniatins in small amounts.
Article
Food Science & Technology
Gabrieli Bernardi, Magali Kemmerich, Franciele F. Machado, Osmar D. Prestes, Martha B. Adaime, Renato Zanella
Summary: A fast and straightforward method based on solvent demulsification-dispersive liquid-liquid microextraction (SD-DLLME) sample preparation was developed and successfully applied for the multiresidue determination of 30 fungicides in wine. The method showed high accuracy and was suitable for commercial wine samples.
FOOD ANALYTICAL METHODS
(2022)
Article
Multidisciplinary Sciences
Mariel Cina, Maria del Valle Ponce, Luis Dante Martinez, Soledad Cerutti
Summary: The evaluation of OTA in tea beverage samples using DLLME-SFO extraction and UHPLC-MS/MS analysis method resulted in increased recovery rate and a high greenness profile according to the Green Certificate comparison with other techniques.
Article
Chemistry, Applied
Adriana Bochetto, Nora Merino, Marcos Kaplan, Maria Guinez, Soledad Cerutti
Summary: An optimized methodology was developed for the determination of mycotoxins in Amaranthus-based samples, achieving efficient and accurate results through liquid chromatography-tandem mass spectrometry. The proposed approach demonstrated high extraction recoveries, enrichment factors, and low detection limits, making it a sensitive and reliable method for analyzing deoxynivalenol and zearalenone levels in mentioned food samples.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Review
Pharmacology & Pharmacy
Abdul Haseeb, Hani Saleh Faidah, Saleh Alghamdi, Amal F. Alotaibi, Mahmoud Essam Elrggal, Ahmad J. Mahrous, Safa S. Almarzoky Abuhussain, Najla A. Obaid, Manal Algethamy, Abdullmoin AlQarni, Asim A. Khogeer, Zikria Saleem, Muhammad Shahid Iqbal, Sami S. Ashgar, Rozan Mohammad Radwan, Alaa Mutlaq, Nayyra Fatani, Aziz Sheikh
Summary: This systematic review analyzed the dosing regimen of beta-lactams and found that continuous infusion is considered the most appropriate mode of administration to optimize the safety and efficacy of the treatment. The study also highlighted the challenges in appropriate antibiotic therapy due to age-related pathophysiological changes, but suggested that optimizing pharmacokinetic/pharmacodynamic parameters can support alternative dosing regimens.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Applied
Akyildiz Ismail Emir, Yetimoglu Kok Ece, Raday Sinem, Acar Sezer, Erdem Ozge
Summary: Novel rapid and breakthrough analysis methods were developed with minimized sample preparation processes, providing practical, low-cost, and time-effective analysis with improved sensitivity. The 'dilute & shoot' and modified QuEChERS methods enabled simultaneous quantification of 21 quinolones in honey, identifying residues of quinolones such as enrofloxacin, danofloxacin, pipemidic acid, lomefloxacin, cinoxacin, and ciprofloxacin.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Chemistry, Applied
Wei Jia, Min Zhang, Rong Zhang, Mudan Xu, Meng Xue, Wangcheng Song, Lin Shi
Summary: This study investigated the effects of homogenization on the distribution of fatty acids and phosphatidylcholine metabolism in goat milk. Results showed that homogenization increased phosphatidylcholine content and altered the composition of diacylglycerols. The relationship between homogenization, milk fat globule recombination, and phospholipids bioaccessibility was preliminarily established.
Article
Chemistry, Applied
Xinhao Zhang, Haijing Li, Li Yang, Guimiao Jiang, Chuanliang Ji, Qin Zhang, Fuwei Zhao
Summary: The study compared the lipidomics profiles of donkey milk, human milk, and cow milk, revealing differences in phospholipid composition, carbon number, and triglyceride unsaturation. Significant different lipids were found between donkey milk and human milk, as well as between donkey milk and cow milk, involving multiple metabolic pathways. These results provide insights into lipid composition, biosynthesis, and metabolic processes, with potential implications for the development of infant formula milk powder or other milk products.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Chemistry, Multidisciplinary
Anna Roszkowska, Natalia Treder, Alina Plenis, Natalia Miekus, Ilona Oledzka, Piotr Kowalski, Tomasz Baczek
Summary: This study compared and optimized two microextraction strategies for the extraction of four immunosuppressants in biofluids. The results showed that one strategy exhibited better performance for the extraction of a novel immunosuppressive drug.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Article
Biochemistry & Molecular Biology
Ovokeroye A. Abafe, Linda R. Macheka, Joshua O. Olowoyo
Summary: An ultra-high performance liquid chromatography tandem mass spectrometry method was developed and validated for the sensitive determination and unambiguous confirmation of residues of per and polyfluorinated alkyl substances (PFAS) in breastmilk, retail milk and infant formulas. The method, using a simplified QuEChERS method for sample pre-treatment, showed good linearity, recovery and within-laboratory reproducibility. The method is suitable for the sensitive and high-throughput confirmatory analysis of C-4-C-14 PFAS in breastmilk, dairy milk and infant formulas.
Article
Chemistry, Applied
Siou Bang Ye, Ying Huang, Ding-Yan Lin
Summary: A method combining QuEChERS technique with ultra-performance liquid chromatography-tandem mass spectrometry was developed and validated for the rapid determination of 19 quinolones in goat's milk. The method selected parameters that best promote extraction efficiency using Plackett-Burman and Central Composite Design methods. The developed method showed high specificity, linearity, recovery rates, repeatability, and intermediate precision, making it suitable for routine analysis of quinolone residues in goat's milk.
Article
Chemistry, Analytical
Zafar Iqbal Tanveer, Qingwen Huang, Tengfei Xu, Yong Chen, Xuesong Liu, Zheng Han, Yongjiang Wu
Summary: In this study, rGO-Zn/Fe2O4 nanomaterial was used as a solid phase extraction adsorbent for the enrichment and separation of aflatoxins from milk. The optimized dispersive solid-phase extraction method combined with UHPLC-MS/MS analysis showed excellent selectivity, a robust linear relationship, and high sensitivity for the detection of aflatoxins in milk.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Jia Zhan, Xi-Zhi Shi, Yi Ding, Ning Qian, Jie Zhou, Shao-Dong Xie, Guo-Zhou Cao, Xian-Feng Chen
Summary: A generic, rapid, simple, and low-cost analytical method for screening and confirming 400 veterinary drugs and other contaminants in raw honey was developed. The method employs one-step extraction and ultra-high performance liquid chromatography coupled with electrospray ionization and tandem mass spectrometry, providing good extraction efficiency and analysis accuracy.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemistry & Molecular Biology
Piotr Cybulski, Anna Gajda, Magdalena Bilecka, Artur Jablonski
Summary: This study aimed to analyze the concentration of tiamulin in sow milk and determine the level of tiamulin in serum samples of suckling piglets. The results demonstrated that tiamulin can be transmitted into sow milk, and the concentration can be relatively high.
Article
Biology
Ilaria Dutto, Julian Gerhards, Antonio Herrera, Olga Souckova, Vaclava Skopova, Jordann A. Smak, Alexandra Junza, Oscar Yanes, Cedric Boeckx, Martin D. Burkhalter, Marie Zikanova, Sebastian Pons, Melanie Philipp, Jens Luders, Travis H. Stracker
Summary: ADSL deficiency causes neurodevelopmental pathologies, including microcephaly and autism spectrum disorder. Reduced purine levels and impaired de novo purine synthesis contribute to the pathology, while defective ciliogenesis may influence the phenotypic spectrum of ADSLD.
Article
Biochemical Research Methods
Inmaculada Moscoso-Ruiz, Yolanda Galvez-Ontiveros, Maria Giles-Mancilla, Maria Del Carmen Gomez-Regalado, Ana Rivas, Alberto Zafra-Gomez
Summary: The presence of endocrine-disrupting chemicals in our daily life is increasing, affecting human exposure and health. A new method for accurate and sensitive analysis of bisphenol homologues and parabens in human urine is proposed. The method has higher extraction efficiency and can calculate matrix effect. Validation results show low limits of detection and quantification, as well as good recovery rates.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
D. Martin-Way, I. Puche-Sanz, J. M. Cozar, A. Zafra-Gomez, M. D. C. Gomez-Regalado, C. M. Morales-Alvarez, A. F. Hernandez, L. J. Martinez-Gonzalez, M. J. Alvarez-Cubero
Summary: Bladder cancer is one of the most common tumors globally, and this study aims to identify molecular markers and risk factors associated with it. The study found several genes associated with bladder cancer risk, as well as environmental exposures and dietary chemicals that contribute to the disease. The study also identified specific miRNA expression patterns that are elevated in bladder cancer cases. These findings suggest the potential use of non-invasive biomarkers and personalized medicine for bladder cancer management.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Analytical
Inmaculada Moscoso-Ruiz, Alberto Navalon, Ana Rivas, Alberto Zafra-Gomez
Summary: Due to their antimicrobial properties, parabens are widely used in food and cosmetics as preservatives. This study evaluated the presence of parabens in children's stool samples and developed an optimized analytical method for their detection. The method successfully identified paraben contamination in 100% of the samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Review
Biochemistry & Molecular Biology
Maria del Carmen Gomez-Regalado, Laura Martin-Pozo, Julia Martin, Juan Luis Santos, Irene Aparicio, Esteban Alonso, Alberto Zafra-Gomez
Summary: There is increasing scientific evidence that certain pharmaceuticals are present in marine ecosystems at concentrations that can have adverse effects on the organisms that inhabit them. However, there is currently limited scientific literature on the accumulation of these compounds in different species, as well as the relationship between their presence and the adverse effects they cause. Efforts have been made to optimize and validate analytical methods for determining pharmaceutical residues in marine organisms through the study of sample treatment, cleanup, and subsequent analysis. This literature review provides a summary of commonly used techniques and their analytical features for determining pharmaceutical compounds in aquatic organisms at various trophic levels over the past 10 years.
Review
Environmental Sciences
Maria del Carmen Gomez-Regalado, Julia Martin, Juan Luis Santos, Irene Aparicio, Esteban Alonso, Alberto Zafra-Gomez
Summary: There is growing evidence that certain pharmaceuticals in the environment pose a potential risk to ecosystems through biota exposure. This review aims to summarize the research and factors involved in assessing the bioaccumulation of pharmaceuticals in aquatic organisms, and provide a critical review of the bioconcentration and bioaccumulation factors of these compounds in different trophic levels. The most commonly studied pharmaceuticals are antidepressants, followed by diclofenac and carbamazepine. Field studies yield more reliable data than laboratory tests for assessing the true environmental impact.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Morsina Cipa, Maria Del Carmen Gomez-Regalado, Alberto Navalon, Elda Marku, Alberto Zafra-Gomez
Summary: This study evaluated the occupational exposure to PAHs in a specific petroleum refinery in Albania. The main exposure route was inhalation in a highly contaminated work environment. Serum samples from workers were collected and analyzed. A cloud-point extraction method was used to isolate and concentrate PAHs from samples. Gas chromatography/mass spectrometry analysis showed that the PAHs present in the serum were primarily pyrogenic PAHs. The bioaccumulation trend of the PAHs in the workers was found to be dependent on their physical location within the workplace.
ATMOSPHERIC POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Maria del Carmen Gomez-Regalado, Julia Martin, Felix Hidalgo, Juan Luis Santos, Irene Aparicio, Esteban Alonso, Alberto Zafra-Gomez
Summary: The bioconcentration kinetics of atenolol and carbamazepine in marine organisms were assessed under controlled laboratory conditions. Carbamazepine showed higher uptake and excretion rates, resulting in a higher bioconcentration factor (BCF), while both were below the limits set by the European Union. The measured BCF using kinetic data showed differences compared to those predicted using steady-state concentrations, possibly due to the steady state not being reached. Specific to the organisms, atenolol exhibited different bioconcentration patterns in different species, with Holothuria tubulosa having the highest BCF. The study also highlighted tissue differences within Holothuria tubulosa. This work is the first to model the bioconcentration of atenolol and carbamazepine in holothurians and anemones.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Maria del Carmen Gomez-Regalado, lado a Laura Martin-Pozo, Felix Hidalgo, Samuel Cantarero-Malagon, Alberto Zafra-Gomez
Summary: Antibiotics are widely used and their presence in the environment is a concern due to the development of antibiotic resistance. This study developed an analytical method to quantitatively analyze antibiotic residues in Holothuria tubulosa specimens. The method involved ultrasound-assisted extraction and dispersive solid phase extraction. The results showed the presence of antibiotics, with ciprofloxacin being the most prevalent, in specimens collected along the Mediterranean coast of Granada.
MICROCHEMICAL JOURNAL
(2023)
Article
Nutrition & Dietetics
Inmaculada Moscoso-Ruiz, Yolanda Galvez-Ontiveros, Cristina Samaniego-Sanchez, Vega Almazan Fernandez de Bobadilla, Celia Monteagudo, Alberto Zafra-Gomez, Ana Rivas
Summary: This study investigated the association between parabens exposure and childhood obesity and found no significant relation between children's body weight and the presence of parabens in their bodies.
Article
Food Science & Technology
Sara Pantalone, Vito Verardo, Alberto Zafra-Gomez, Eduardo Guerra-Hernandez, Angelo Cichelli, Nicola D'Alessandro, Ana Maria Gomez-Caravaca
Summary: The study aimed to investigate the impact of different oils (EVOO, refined olive oil, HOSO, and RSO) and intermittent deep-frying on the levels of GD, MCPDs, and AA in pre-fried French fries. The results showed that the content of MCPDs, GD, and AA were influenced by the time and type of oil used. The migration of MCPDs and glycidols in French fries during frying was observed, along with the formation of new compounds. EVOO exhibited the lowest MCPDs and GD content, while HOSO resulted in the lowest AA levels.
Article
Chemistry, Analytical
Maria del Carmen Gomez-Regalado, Lydia Espin-Moreno, Laura Martin-Pozo, Alberto Zafra-Gomez
Summary: This study compares the efficiency of ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) in determining 17 commonly prescribed antibiotics in human nails. After optimizing the extraction parameters using multivariate techniques, MAE was found to be more practical and provided better extraction efficiencies. The optimized method was then applied to nail samples from 10 volunteers, revealing the presence of one or more antibiotics in all samples, with sulfamethoxazole, danofloxacin, and levofloxacin being the most commonly found.
Article
Nutrition & Dietetics
Yolanda Galvez-Ontiveros, Inmaculada Moscoso-Ruiz, Vega Almazan Fernandez de Bobadilla, Celia Monteagudo, Rafael Gimenez-Martinez, Lourdes Rodrigo, Alberto Zafra-Gomez, Ana Rivas
Summary: This study investigated the association between Bisphenol A (BPA) and 11 BPA analogs in children and overweight/obesity. The results suggested that BPF in nails was associated with overweight/obesity in children, while the highest concentrations of BPAF in saliva showed an inverse association. No significant associations were found between exposure to BPA or its analogs and overweight/obesity in other biological matrices.
FRONTIERS IN NUTRITION
(2023)
Article
Engineering, Environmental
Fatine El Moukhtari, Laura Martin-Pozo, Alberto Zafra-Gomez
Summary: The continuous contamination of the environment by a high variety of pollutants is a global concern. Among these pollutants, endocrine disrupting chemicals (EDCs) are particularly problematic due to their persistence and toxicity. Conventional treatments are not sufficient to remove EDCs, making bioremediation a promising alternative. Microbial communities, enzymes, and fungi have shown potential in efficiently transforming EDCs into non-hazardous end products. Certain aerobic bacteria such as Sphingomonas, Achromobacter, and Pseudomonas have proven to be highly effective in removing EDCs such as nonylphenols, bisphenols, and parabens.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Agriculture, Multidisciplinary
Alexandra Junza, Javier Saurina, Cristina Minguillon, Dolores Barron
Summary: LC-HRMS techniques were used to study metabolomic profile changes in milk from cows with mastitis and treated with enrofloxacin. Principal component analysis was applied to identify changes in compounds unrelated to enrofloxacin. The majority of compounds affected by the pharmacological treatment and/or pathological status were amino acids and peptides. Potential biomarkers for disease or treatment were detected, and the impact of thermal processes on the identified metabolites was also considered.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)