Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1364, Issue -, Pages 271-275Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2014.08.069
Keywords
Thin-layer chromatography; Inductively coupled plasma mass spectrometry; Diode laser thermal vaporization; Hyphenated techniques; Speciation; Cobalamin
Funding
- Czech Science Foundation [GAP206/12/0538]
- project CEITEC from the European Regional Development Fund [CZ.1.05/1.1.00/02.0068]
- Program of Employment of Newly Graduated Doctors of Science for Scientific Excellence - European Social Fund [CZ.1.07/2.3.00/30.0009]
- Czech Republic
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Here we present a novel coupling of thin-layer chromatography (TLC) to diode laser thermal vaporization inductively coupled plasma mass spectrometry (DLTV ICP MS). DLTV is a new technique of aerosol generation which uses a diode laser to induce pyrolysis of a substrate. In this case the cellulose stationary phase on aluminum-backed TLC sheets overprinted with black ink to absorb laser light. The experimental arrangement relies on economic instrumentation: an 808-nm 1.2-W continuous-wave infrared diode laser attached to a syringe pump serving as the movable stage. Using a glass tubular cell, the entire length of a TLC separation channel is scanned. The 8-cm long lanes were scanned in similar to 35 s. The TLC - DLTV ICP MS coupling is demonstrated on the separation of four cobalamins (hydroxo-; adenosyl-; cyano-; and methylcobalamin) with limits of detection similar to 2 pg and repeatability similar to 15% for each individual species. (C) 2014 Elsevier B.V. All rights reserved.
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