☆ 4.6 Article

Influence of the nature of alkyl substituents on the high-performance liquid chromatography enantioseparation and retention of new atropisomeric 1,1′-bibenzimidazole derivatives on amylose tris(3,5-dimethylphenylcarbamate) chiral stationary phase

JOURNAL OF CHROMATOGRAPHY A (2014)

Journal

JOURNAL OF CHROMATOGRAPHY A

Volume 1363, Issue -, Pages 128-136

Publisher

ELSEVIER SCIENCE BV

DOI: 10.1016/j.chroma.2014.08.060

Keywords

Enantiomer separation; Chiral stationary phase; Chiralpak AD-3; Enantiomer elution order; Isoenantioselective temperature; Thermodynamic parameters

Categories

Biochemical Research Methods Chemistry, Analytical

Funding

Fondazione Cariplo [2011-1851]

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Abstract

Six new atropisomeric heteroarenes were synthesized by connecting two 2-alkylbenzimidazole fragments via N-N junction. They differ by the substituent nature (methyl, ethyl, propyl, butyl, pentyl and hexyl) of the aliphatic function. The novel atropisomeric compounds were used as chiral probes to study the chromatographic behavior of the amylose tris(3,5-dimethylphenyl carbamate) (Chiralpak AD-3) chiral stationary phase (CSP) under normal phase mode. The pivotal role of the length and flexibility of the 2,2'-alkyl groups on retention, enantioselectivity and enantiomer elution order was demonstrated by enantioselective HPLC analysis. Additional information on the chiral recognition mechanism was obtained from the evaluation of the correlated thermodynamic data. (C) 2014 Elsevier B.V. All rights reserved.

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Article Biochemical Research Methods

A two-run heart-cut multidimensional gas chromatography method using flame ionization and mass spectrometry for automated and robust determination of nearly complete wine aroma-volatile profiles

Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira

Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.

JOURNAL OF CHROMATOGRAPHY A (2024)

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