Article
Biochemistry & Molecular Biology
Shuang-Qing Zhang, Sui-Han Cheng, Shi Shen, Bi-Ying Luo, Yan Zhang
Summary: A new method was developed for quantifying Cr(III) and Cr(VI) in Chromium-enriched yeast, using ion chromatography-inductively coupled plasma mass spectrometry. Cr(III) and Cr(VI) were extracted using ultrasound-assisted weakly alkaline EDTA solution combined with boiling, and separated on two successive anion-exchange columns. The method was sensitive, accurate, and precise, with no Cr(VI) species found in CrY.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Kittiya Plaeyao, Ratchaneekorn Kampangta, Yuparat Korkokklang, Chanon Talodthaisong, Apichart Saenchoopa, Saengrawee Thammawithan, Krailikhit Latpala, Rina Patramanon, Navaphun Kayunkid, Sirinan Kulchat
Summary: This study synthesized ginger-stabilized silver nanoparticles (Gin-AgNPs) using an eco-friendly method and demonstrated their application in the detection of Hg2+ in tap water. The colorimetric sensor showed good sensitivity, with a LOD of 1.46 μM and a LOQ of 3.04 μM, and was not affected by other metal ions. Machine learning approach improved the performance of the sensor, achieving accuracy ranging from 0% to 14.66% in detecting different concentrations of Hg2+. Furthermore, the Gin-AgNPs and Gin-AgNPs hydrogels exhibited antibacterial effects, suggesting potential applications in Hg2+ detection and wound healing.
Article
Chemistry, Analytical
Suvi Kulomaki, Elmeri Lahtinen, Siiri Peramaki, Ari Vaisanen
Summary: A simple and sensitive method utilizing 3D printed metal scavenger for preconcentration and speciation of mercury species in water samples has been developed. By studying experimental parameters, MeHg and iHg concentrations in water samples were successfully determined with high analytical accuracy.
Article
Chemistry, Applied
Zhiming Xiao, Jitong Wang, Jiangpeng Guo, Decheng Suo, Shi Wang, Jing Tian, Lili Guo, Xia Fan
Summary: The newly developed analytical method is rapid, sensitive, and species-preservative, allowing for the simultaneous determination of multiple selenium species. It has been successfully applied to various reference materials and feed samples for selenium speciation analysis.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2021)
Article
Biochemical Research Methods
Maria S. Jimenez, Mariam Bakir, Daniel Isabal, Maria T. Gomez, Josefina Perez-Arantegui, Juan R. Castillo, Francisco Laborda
Summary: In this study, hydrodynamic chromatography coupled to inductively coupled plasma mass spectrometry was used to simultaneously determine dissolved and nanoparticulate species of gold and silver, with optimized mobile phase for resolution and recovery. The developed methods allowed for quick and accurate detection of nanoparticulate and dissolved species of gold and silver in dietary supplements, showing good agreement with other analysis methods. Due to achievable resolution, the quality of quantitative results is influenced by the relative abundance and size of nanoparticles present.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Environmental
Shaochen Yang, Ping Li, Kaifeng Sun, Nan Wei, Jinling Liu, Xinbin Feng
Summary: Anthropogenic activities and climate change have led to increased mercury (Hg) levels in seawater, but the sources and processes of Hg in different marine compartments are not well understood. This study analyzed Hg concentrations and stable isotopes in seawater and fish samples collected from different marine compartments of the South China Sea to investigate Hg cycling in marine ecosystems. The results showed that estuarine seawater had higher Hg concentrations than marine continental shelf and pelagic seawater, and the sources of Hg varied in different compartments. This study highlights the importance of Hg stable isotopes for understanding Hg cycling in marine ecosystems.
Article
Chemistry, Analytical
Alina Kleindienst, Igor Zivkovic, Emmanuel Tessier, Alkuin Koenig, Larseric Heimburger-Boavida, Milena Horvat, David Amouroux
Summary: By assessing the measurement uncertainties, we validated the application of an indirect method for determining DMHg and suggested the potential for generating more precise and accurate DMHg data.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Melissa Gilbert, Peng Ho, Laura Whitmore, Alan Shiller
Summary: There is a growing interest in dissolved gallium (Ga) in natural waters for tracking water masses, but current analytical methods are time-consuming and labor-intensive. In this study, an automated isotope dilution method was developed to determine Ga in seawater using commercially available equipment. The method addressed challenges such as sample volume and pre-concentration, instrumental interferences, sample carryover, sample recovery variability, and improved detection limits, accuracy, and precision. The new automated method showed comparable Ga concentrations to traditional methods but with greater precision and a lower detection limit.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Kamal K. Jinadasa, Paloma Herbello-Hermelo, Elena Pena-Vazquez, Pilar Bermejo-Barrera, Antonio Moreda-Pineiro
Summary: A pre-concentration method for low mercury levels in seaweed was developed, utilizing an imprinted polymer for selective extraction and determination of mercury species. The method showed low detection limits, good repeatability, and was successfully applied to certified reference materials and edible seaweeds for analysis of methylmercury and Hg(II).
Article
Chemistry, Analytical
Yihuan Song, Qingfang Ma, Heyong Cheng, Jinhua Liu, Yuanchao Wang
Summary: A method utilizing online solid-phase extraction coupled with high performance liquid chromatography and inductively coupled plasma mass spectrometry was developed for simultaneous enrichment and analysis of ultra-trace lead and mercury species in water. This method allows for efficient and accurate determination of lead and mercury species in water samples, with high enrichment factors and low LODs and LOQs. The method was successfully applied to analyze trace lead and mercury species in various types of water samples, showing promising results for unstable sample analysis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Limnology
Chikako Cheong, Tsutomu Miura, Naoko Nonose
Summary: A novel method was developed for the analysis of phosphate in seawater using ICP-SFMS and IC, enhancing P-31 sensitivity by optimizing instrument settings and adding an internal standard element. The method successfully corrected fluctuations in sensitivity and allowed for accurate and sensitive measurement of phosphate, with results consistent with continuous flow analysis.
LIMNOLOGY AND OCEANOGRAPHY-METHODS
(2021)
Article
Biochemical Research Methods
M. Ribeiro, E. Galli, T. Guerin, J. A. L. Silva, I. Castanheira, A. Leufroy, P. Jitaru
Summary: This study presents the development and validation of a new analytical method for simultaneous speciation analysis of selenium and mercury in fish muscle. The method involves the extraction and separation of the different species using microwave-assisted enzymatic hydrolysis and HPLC with a specific column and mobile phase. The method was validated and successfully applied to analyze muscle samples from various fish species.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Magadima Mary Thosago, Angelique Botha, Abayneh Ataro Ambushe, Taddese Wondimu Godeto
Summary: A method for arsenic speciation in South African wheat flour was developed using microwave-assisted extraction with ion chromatography separation and detection with high resolution inductively coupled plasma-mass spectrometry. The method was optimized and validated, and the results showed that the levels of inorganic arsenic in the wheat flour were below the permissible level proposed by the World Health Organization.
ANALYTICAL LETTERS
(2022)
Article
Biochemical Research Methods
Catharina Erbacher, Nils Flothkoetter, Marcel Macke, C. Derrick Quarles, Michael Sperling, Jens Mueller, Uwe Karst
Summary: A fast and automated method for separation and quantification of bromide and 5-BrdU via IC and ICP-MS was developed, relevant for monitoring electron transfer efficiency along DNA. With a LOD of 30 ng/L, the method is suitable for screening applications with a multitude of samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Spectroscopy
Peng Li, Yu-Ying Huang, Jun Zeng, Zheng-Zhong Lin, Zhi-Yong Huang
Summary: The study successfully investigated cadmium species in marine shellfish using SEC-ICP-MS and SEC-ESI-Q-TOF-MS methods, revealing multiple cadmium binding species in clams, including high molecular weight Cd-binding molecules and MTs-like Cd-binding proteins.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2021)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)