Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1271, Issue -, Pages 192-200Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2012.11.034
Keywords
Mineral oil saturated hydrocarbons (MOSH); Mineral oil aromatic hydrocarbons (MOAH); Silver nitrate/silica gel; Separation from wax esters; Toluene to support selective reconcentration
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So far the majority of the measurements of mineral oil saturated hydrocarbons (MUSH) and mineral oil aromatic hydrocarbons (MOAH) were obtained from on-line high performance liquid chromatography-gas chromatography-flame ionization detection (on-line HPLC-GC-FID). Since this technique is not available in many laboratories, an alternative method with more easily available tools has been developed. Preseparation on a small conventional liquid chromatographic column was optimized to achieve robust separation between the MOSH and the MOAH, but also to keep out the wax esters from the MOAH fraction. This was achieved by mixing a small portion of silica gel with silver nitrate into highly activated silica gel and by adding toluene into the eluent for the MOAH. Toluene was also added to the MOSH fraction to facilitate reconcentration and to serve as a keeper preventing loss of volatiles during solvent evaporation. A 50 mu l volume was injected on-column into GC-FID to achieve a detection limit for MUSH and MOAH below 1 mg/kg in most foods. (C) 2012 Elsevier B.V. All rights reserved.
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