Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1283, Issue -, Pages 62-67Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2013.01.085
Keywords
Methotrexate; Folic acid; Functionalized multi-wall carbon nanotubes; Ion chromatography; Electrochemical detection
Funding
- National Natural Science Foundation of China [20775070]
- Zhejiang Provincial Natural Science Foundation of China [Y407153, Y4100190]
- Zhejiang Provincial Assay Foundation of China [2012 C37003]
Ask authors/readers for more resources
A simple and sensitive method was developed for simultaneous determination of methotrexate (MTX) and folic acid (FA) by ion chromatography with electrochemical detection (IC-ECD). Quaternary amine functionalized multi-wall carbon nanotubes (q-MWNTs) modified glassy carbon electrode (GCE) was used as an amperometric sensor to determine MTX and FA. The electrochemical behaviors of MTX and FA at the q-MWNTs/GCE were studied by cyclic voltammetry (CV). Results indicated that this modified electrode exhibited electrocatalytic oxidation toward MTX and FA with high sensitivity, stability and long life. Good separation of MTX and FA was demonstrated by IC on an anion-exchange column with phosphate buffer solution (PBS) as eluent. Under the optimal conditions, the linear ranges were from 0.01 to 20 mg/L for both MTX and FA with correlation coefficients r >= 0.9994. The obtained detection limits (LODs) for MTX and FA were 0.2 and 0.4 mu g/L (S/N = 3), respectively. The method has been successfully applied to the determination of MTX and FA in plasma and urine of patients of rheumatoid arthritis. (c) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available