Article
Biophysics
Joao Carlos Simoes-Cardoso, Nanako Hoshino, Yusuke Yoshimura, Chyi-Shin Chen, Cristina Dias-Cabral, Noriko Yoshimoto, Shuichi Yamamoto
Summary: This study investigates the thermodynamics of bovine serum albumin monomer adsorption onto chromatographic surfaces with grafted ligands, revealing clear differences in the driving forces of adsorption caused by different ligand architectures. Additionally, a new relation between the salt required for protein elution and the change in adsorption enthalpy is found, intrinsically connecting both adsorption and desorption mechanisms.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Thermodynamics
Ziga Medos, Isidora Cobanov, Marija Bester-Rogac, Bojan Sarac
Summary: This research paper examines the issues associated with power compensation isothermal titration calorimetry, highlighting the possibility of obtaining different enthalpy values with different instruments and setting parameters. It emphasizes that modern calorimeters' dynamic power compensation mode may lead to overcompensation of heat effects, resulting in experimental results heavily reliant on chosen parameters. To avoid these problems, the paper suggests checking raw signals and conducting experiments with low or disabled power compensation when observing switching effects.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2021)
Article
Chemistry, Physical
Pablo F. Garrido, Margarida Bastos, Adrian Velazquez-Campoy, Alfredo Amigo, Philippe Dumas, Angel Pineiro
Summary: The injection of air into an isothermal titration calorimeter's sample cell allows for accurate determination of the surface tension of the target liquid, despite the periodic contribution from bubbles. The new algorithm successfully identifies and analyzes these contributions, improving the analysis of calorimetric signals.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jean Nyoung Yun, Junseock Koh
Summary: Understanding the molecular mechanisms of multi-domain proteins and intrinsically disordered proteins undergoing liquid-liquid phase separation to form membraneless organelles or protein condensates is crucial. However, the regulatory functions of these condensates are still unclear due to limited knowledge about their assembly driving forces. In this study, isothermal titration calorimetry (ITC) is proposed as an efficient analytical tool to explore the thermodynamic parameters of cooperative condensate formations. Systematic simulations demonstrate that the initial heat analysis can quantitatively dissect protein condensates with a broad range of thermodynamic properties. This provides an opportunity to investigate the molecular driving forces of liquid-liquid phase separation by scrutinizing the thermodynamic quantities as functions of solution variables.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Fanrui Sha, Tzu-Yi Tai, Madeleine A. Gaidimas, Florencia A. Son, Omar K. Farha
Summary: Isothermal titration calorimetry (ITC) is a technique for measuring the thermodynamic parameters of binding events, which has applications not only in biological macromolecules but also in synthetic systems like metal-organic frameworks (MOFs). Utilizing ITC in MOFs can provide insights into small molecule adsorption mechanisms and the formation of MOF-based composite materials through non-covalent interactions, with specific experimental considerations discussed. Furthermore, underexplored research directions related to MOFs that could benefit from ITC are highlighted.
Article
Thermodynamics
Federica D'Aria, Bruno Pagano, Concetta Giancola
Summary: Cyclodextrins (CDs) have been shown to improve the stability, solubility, and bioavailability of various molecules, with low toxicity for different routes of administration. Isothermal titration calorimetry (ITC) is a key method for studying cyclodextrin/guest interaction, providing important thermodynamic parameters simultaneously.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2022)
Article
Physics, Multidisciplinary
Monica Corea, Rogelio Jimenez-Juarez, Gabriela Martinez-Mejia, Maria de Jesus Martinez-Ortiz, Jose Manuel del Rio
Summary: The study experimentally analyzed the influence of the stock solution concentration on the geometrical characteristics of the titration curve, and found that the magnitude and shape of the heat titration depend on the stock solution concentration until reaching a limiting value. Qualitative analysis suggested that the limiting value is substance-dependent.
Article
Construction & Building Technology
K. -D. Grevel, F. Bellmann, J. Majzlan, E. Dachs, A. Benisek, H. -M. Ludwig
Summary: The study investigated the crystalline phases of Ca2SiO4 in Portland cement clinker through calorimetry, recording and comparing low-temperature heat capacity data. Recommended values for beta-C2S and gamma-C2S were obtained.
CEMENT AND CONCRETE RESEARCH
(2022)
Article
Engineering, Environmental
Min-Ji Kim, Yuliia Herchenova, Jaeshik Chung, Sang-Heon Na, Eun-Ju Kim
Summary: This study systematically investigates the aggregation behavior of polystyrene nanoplastics (PS NPs) under different electrolyte conditions and the impact of environmental factors on NP stability. The results show that solvation entropy plays a significant role in NP aggregation, and the stability of PS NPs is influenced by ionic strength and the presence of natural colloids.
Article
Environmental Sciences
Hongfeng Chen, Mingxia Hou, Zhouyang He, Yu Liang, Jinling Xu, Wenfeng Tan
Summary: This study investigated the complex species of fulvic acid on the surface of minerals (kaolinite and goethite) and their thermodynamic characteristics. The adsorption of kaolinite for fulvic acid increased at pH < 5.5 and decreased at pH > 5.5, while that of goethite consistently decreased with increasing pH. Fulvic acid was mainly adsorbed on the edge-face and O-face to form inner sphere complexes on kaolinite and outer sphere complexes on goethite. The molar adsorption enthalpies and complexation mechanisms differed between kaolinite and goethite.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Physical
Pablo F. Garrido, Margarida Bastos, Adrian Velazquez-Campoy, Philippe Dumas, Angel Pineiro
Summary: The experiment focused on the spontaneous adsorption of amphiphilic molecules to fluid interfaces and the heat exchange involved in the process. Findings showed that the formation, growth, and release of air bubbles in different liquids resulted in various heat phenomena, offering insights into parameters such as surface tension.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Shams T. A. Islam, Jie Zhang, Fabio Tonin, Renske Hinderks, Yanthi N. Deurloo, Vlada B. Urlacher, Peter-Leon Hagedoorn
Summary: Lignin valorization through laccase catalysis can release aromatic products, but different enzymes exhibit significant variations in conversion efficiency and rate. Trametes versicolor laccase follows Michaelis-Menten kinetics in converting Kraft lignin, with specific thermodynamic and kinetic parameters, making it a promising tool for guiding enzymatic lignin valorization strategies.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Chemistry, Applied
Wenfei Xiong, Ya Li, Cong Ren, Jing Li, Bin Li, Fang Geng
Summary: Investigating the thermodynamic parameters in the formation process of protein-polysaccharide complex coacervates is crucial for understanding the binding mechanism. pH, salt ion strength, and temperature were found to influence the thermodynamic parameters of gelatin (G)-pectin (P) complex coacervation. The binding between G and P involves electrostatic bonding, hydrogen bonding, and hydrophobic interactions.
FOOD HYDROCOLLOIDS
(2021)
Review
Pharmacology & Pharmacy
Iago Dillion Lima Cavalcanti, Francisco Humberto Xavier Junior, Nereide Stela Santos Magalhaes, Mariane Cajuba de Britto Lira Nogueira
Summary: Isothermal titration calorimetry (ITC) is a technique for evaluating the thermodynamic profiles of connection between two molecules, allowing the experimental design of nanoparticles systems with drugs and/or biological molecules. In the field of pharmaceutical nanotechnology, ITC has been increasingly used to understand the interaction mechanisms in the formation of nanoparticles and the behavior of nanocarriers in in vivo studies. This technique provides quick and easy results to optimize the formulation process of nano-systems.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Thermodynamics
Vitaly Kocherbitov
Summary: The sorption calorimetry method developed by Wadso and co-workers is successful in measuring both water sorption isotherm and sorption enthalpy, though accuracy can be affected by diffusion. A new calculation method is proposed to eliminate these effects and improve the accuracy of enthalpy measurements. This new method has been successfully tested on previously published data and can be recommended for future experiments.
JOURNAL OF CHEMICAL THERMODYNAMICS
(2021)
Article
Engineering, Chemical
Agnes Froescher, Kai Langenbach, Erik von Harbou, Hans Hasse
Article
Chemistry, Physical
Martin P. Lautenschlaeger, Martin Horsch, Hans Hasse
Article
Thermodynamics
Elmar Kessler, Luciana Ninni, Tanja Breug-Nissen, Benjamin Willy, Rolf Schneider, Muhammad Irfan, Joern Raker, Erik von Harbou, Hans Hasse
JOURNAL OF CHEMICAL AND ENGINEERING DATA
(2019)
Article
Thermodynamics
Nadia Galeotti, Jakob Burger, Hans Hasse
JOURNAL OF CHEMICAL AND ENGINEERING DATA
(2019)
Article
Chemistry, Physical
Martin P. Lautenschlaeger, Hans Hasse
Article
Engineering, Chemical
Fabian Jirasek, Jakob Burger, Hans Hasse
Article
Engineering, Chemical
Dan Vasiliu, Elmar Kessler, Erik von Harbou, Hans Hasse
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2020)
Article
Thermodynamics
Daniel Bellaire, Hendrik Kiepfer, Kerstin Muennemann, Hans Hasse
JOURNAL OF CHEMICAL AND ENGINEERING DATA
(2020)
Article
Thermodynamics
Alexander Keller, Irenaus Wlokas, Maximilian Kohns, Hans Hasse
JOURNAL OF CHEMICAL AND ENGINEERING DATA
(2020)
Article
Biochemical Research Methods
Anne Friebel, Thomas Specht, Erik von Harbou, Kerstin Muennemann, Hans Hasse
JOURNAL OF MAGNETIC RESONANCE
(2020)
Article
Chemistry, Physical
Fabian Jirasek, Rodrigo A. S. Alves, Julie Damay, Robert A. Vandermeulen, Robert Bamler, Michael Bortz, Stephan Mandt, Marius Kloft, Hans Hasse
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2020)
Article
Chemistry, Physical
Simon Stephan, Hans Hasse
Article
Engineering, Chemical
Nadia Galeotti, Eva Hackemann, Fabian Jirasek, Hans Hasse
SEPARATION AND PURIFICATION TECHNOLOGY
(2020)
Article
Engineering, Multidisciplinary
Felix Diewald, Martin P. Lautenschlaeger, Simon Stephan, Kai Langenbach, Charlotte Kuhn, Steffen Seckler, Hans-Joachim Bungartz, Hans Hasse, Ralf Mueller
COMPUTER METHODS IN APPLIED MECHANICS AND ENGINEERING
(2020)
Article
Physics, Fluids & Plasmas
Simon Stephan, Hans Hasse
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)