Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1285, Issue -, Pages 124-131Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2013.02.039
Keywords
Acyclovir; High performance liquid chromatography; Molecularly imprinted polymer receptor; Hydrogen-bond array
Funding
- National Natural Science Foundation of China [20875034, 21275057]
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This paper demonstrates a novel approach to assembling homogeneous molecularly imprinted polymers (MIPs) based on mimicking multiple hydrogen bonds between nucleotide bases by preparing acyclovir (ACV) as a template and using coatings grafted on silica supports. H-1 NMR studies confirmed the AAD-DDA (A for acceptor, D for donor) hydrogen-bond array between template and functional monomer, while the resultant monodisperse molecularly imprinted microspheres (MIMs) were evaluated using a binding experiment, high performance liquid chromatography (HPLC), and solid phase extraction. The Langmuir isothermal model and the Langmuir-Freundlich isothermal model suggest that ACV-MIMs have more homogeneous binding sites than MIPs prepared through normal imprinting. In contrast to previous MIP-HPLC columns, there were no apparent tailings for the ACV peaks, and ACV-MIMs had excellent specific binding properties with a K-a peak of 3.44x 10(5) M-1. A complete baseline separation is obtained for ACV and structurally similar compounds. This work also successfully used MIMs as a specific sorbent for capturing ACV from serum samples. The detection limit and mean recovery of ACV was 1.8 ng/mL(-1) and 95.6%, respectively, for molecularly imprinted solid phase extraction coupled with HPLC. To our knowledge, this was the first example of MIPs using AAD-DDA hydrogen bonds. (C) 2013 Elsevier B.V. All rights reserved.
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