Article
Engineering, Chemical
Min Chen, Chengna Dai, Gangqiang Yu, Ning Liu, Ruinian Xu, Ning Wang, Biaohua Chen
Summary: This study proposes and systematically investigates the technology for absorbing MTBE with ionic liquids, from the molecular level to system scale. The research demonstrates that ILs are potential absorbents, and IL-based mixed absorbents may be a better strategy for achieving highly efficient absorption of MTBE, reducing product gas loss and energy consumption.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Yuting Wang, Yisheng Wang, Chen Chen, Fang Ren, Rui Cao, Yuefei Wang, Pin Han, Xiaoyan Zhang, Congjian Xu, Xinyu Liu, Guowang Xu
Summary: The study identified lipid disturbances in the serum of patients with epithelial ovarian cancer (EOC) and established 4 lipids as potential markers for aiding EOC diagnosis. These markers showed high accuracy in distinguishing EOC and early EOC from non-cancer, with good specificity and sensitivity. The research not only revealed the characteristics of lipid metabolism in EOC, but also provided a potential marker pattern for aiding EOC diagnosis.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Multidisciplinary
Tingyu Hua, Shanshan Li, Ling Wang, Wei Yan
Summary: In this study, three strains with high adaptability and different degrading characteristics to MTBE were cultured, and kinetic models were established to simulate the biodegradation process of MTBE by the strains at different concentrations. The study found that a mixed consortium of the three strains significantly enhanced the removal efficiency and rate of MTBE, especially at high concentrations. Furthermore, the mixed consortium showed strong adaptability and effectively removed other gasoline components. This research provides support for the use of a mixed consortium to remediate MTBE-contaminated environments.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Analytical
Zhaoxia Shi, Ling Xia, Gongke Li, Yufei Hu
Summary: Pt/Au NPs@Co3O4 composites with a hollow dodecahedron three-dimensional structure were successfully synthesized, and a CTL sensor based on this composite was established for the accurate and rapid detection of MSBE in MTBE. The experimental results demonstrated that the sensor exhibited strong specificity and sensitivity, and the analytical results were consistent with those obtained using traditional gas chromatography.
Article
Engineering, Chemical
Jordi H. Badia, Carles Fite, Roger Bringue, Fidel Cunill, Javier Tejero
Summary: The kinetics of the simultaneous synthesis of ETBE and BTBE over A35 catalyst in the liquid phase was investigated. Various kinetic modeling approaches were compared, and it was found that empirical power-law modeling provided the best fit, while mechanistic modeling explained the reaction mechanisms.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Domenico Masuero, Domen Skrab, Giulia Chitarrini, Mar Garcia-Aloy, Pietro Franceschi, Paolo Sivilotti, Graziano Guella, Urska Vrhovsek
Summary: A new mass spectrometry method was developed in this study, successfully analyzing 412 compounds in grapes. The results showed that certain lipid components were positively correlated with grape maturity, while others were negatively correlated.
Editorial Material
Biochemistry & Molecular Biology
Cosima Damiana Calvano, Ilario Losito, Tommaso Cataldi
Summary: This special issue focuses on the contribution of dysregulation of lipid signaling and metabolism to neurodegenerative diseases, including Alzheimer's and Parkinson's, summarizing three reviews and one research article.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Energy & Fuels
Iwan Ridwan, Charoen Chinwanitcharoen, Kazuhiro Tamura
Summary: The addition of water to supercritical tert-butyl methyl ether enhances biodiesel yield, with the optimal conditions being 25 wt% water addition, 300 degrees C temperature, 20 MPa pressure, and 30 min space time resulting in a 77.39 wt% biodiesel yield. The reaction rate is accelerated by water, acting as a catalyst, and the supercritical reaction is found to be endothermic, endergonic, and non-spontaneous based on thermodynamic parameters.
Article
Engineering, Chemical
Yanli Zhang, Zihao Su, Ke Xue, Jiafu Xing, Dingchao Fan, Jianguang Qi, Zhaoyou Zhu, Yinglong Wang
Summary: This study investigated the efficient separation of MTBE from gasoline using ILs and explored the separation mechanism using quantum chemistry and molecular dynamics. The results showed that 1-butyl-3-methylimidazolium nitrate and 1-ethanyl-3-methylimidazole hydrogen sulfate had the highest extraction rate.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Article
Environmental Sciences
Qiqi Zhu, Shanshan Zhu, Qiyao Li, Yun Wang, Dongxu Zheng, Chunjiang Chen, Naqi Ruan, Haiqiong Chen, Haoni Yan, Han Lin, Ren-Shan Ge
Summary: Exposure to MTBE during late puberty compromises the development of Leydig cells in male rats by inhibiting cell proliferation, inducing mitochondrial autophagy and apoptosis, and indirectly stimulating Sertoli cells to secrete anti-Mullerian hormones, resulting in a reduced number of Leydig cells.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biotechnology & Applied Microbiology
Yiyang Chen, Hao Ren, Xiangyu Kong, Hao Wu, Zhenmei Lu
Summary: In this study, an MTBE-degrading enzyme, prmABCD, was identified and its role in MTBE metabolism was revealed. Transcriptome analysis showed that the expression of prmABCD was upregulated by MTBE. Functional characterization demonstrated that prmABCD could catalyze the degradation of MTBE, and the loss of genes encoding the oxygenase subunits, coupling protein, or reductase disrupted MTBE degradation. The catalytic capacity of PRM was influenced by specific amino acid residues in the active site and substrate tunnel.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ruchika Bhawal, Qin Fu, Elizabeth T. Anderson, Gary E. Gibson, Sheng Zhang
Summary: The study applied a parallel metabolomics and lipidomics approach to investigate the serum metabolome and lipidome of patients treated with BFT, identifying multiple novel biomarkers and potential therapeutic targets involved in glucose metabolism and biosynthesis of aromatic amino acids. Significant differences were found between the placebo and BFT treatment groups in terms of metabolites and lipid species, indicating potential mechanisms underlying the benefits of BFT in Alzheimer's disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Chemical
Xue-Ling Wei, Bo Song, Yu-Ling Luo, Jun-Fang An, Jun-Bin Zhang, Meng Pan, Xingyang Li
Summary: In this study, compact NaX zeolite membranes with excellent pervaporation performance for methanol/MTBE mixtures were synthesized by controlling the deposition process of the silicon aluminum components using water-organic solvent synthesis solutions. This new method provides a new approach for the preparation of compact membranes.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Engineering, Environmental
Yanli Zhang, Ke Xue, Huiyuan Li, Shuyi Lian, Chunyang Han, Zhaoyou Zhu, Yanyue Lu, Jianguang Qi, Yinglong Wang
Summary: In this study, the process of extracting MTBE from petroleum wastewater using a mixed extractant of water and ionic liquids (ILs) was clarified. A comprehensive method of evaluating the extraction performance was proposed using the COSMO-SAC model, liquid-liquid extraction measurement, and micro-scale analysis mechanism. The interaction mechanisms of different solvents with MTBE and ethanol were revealed through molecular dynamics simulations and quantum chemical calculations.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Biochemical Research Methods
Lauren M. Bishop, Oliver Fiehn
Summary: Large-scale lipidomic analyses have been limited by the cost and accessibility of traditional venipuncture sampling. Microsampling techniques offer a less-invasive and more accessible alternative. From a single drop of blood, plasma separation cards (PSC) deliver two volumetric dried plasma samples which are studied here for profiling endogenous blood lipids.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biology
Chao Li, Peng Dou, Tianxiang Wang, Xin Lu, Guowang Xu, Xiaohui Lin
Summary: Studying miRNA synergy and identifying miRNA synergistic modules can help understand complex diseases such as cancers. A new method called DDRM combines knowledge databases and miRNA data to define disease-related modules and construct a weighted miRNA synergistic network. Experiments on TCGA datasets showed that DDRM can accurately distinguish cancer samples from normal samples and outperforms previous methods. Validation on prostate cancer data also demonstrated superior performance. Hence, combining miRNA synergy networks and miRNA data is significant for studying disease mechanisms.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Food Science & Technology
Zhihui Wang, Zhihua Wang, Weidong Dai, Yan Huang, Fuming Lin, Haomin Dai, Zhe Zhou, Xingchen Lin, Zhi Lin, Weijiang Sun
Summary: In this study, the composition and dynamic changes of the bacterial community during the long-term storage of compressed white tea (CWT) were investigated. The results showed that the bacterial diversity initially decreased and then increased significantly during CWT storage. The dominant phyla and genera of bacteria changed significantly in the 10th year, which was also the key period for alterations in antioxidant capability. The study further revealed that aerobic bacteria played a dominant role in these changes and influenced the phenol content, thus affecting the antioxidant ability of CWT. Additionally, 29 biomarkers unique to different storage years were identified during the study.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Miriam Hoene, Xinjie Zhao, Juergen Machann, Andreas L. L. Birkenfeld, Martin Heni, Andreas Peter, Andreas Niess, Anja Moller, Rainer Lehmann, Guowang Xu, Cora Weigert
Summary: Physical exercise is a powerful preventative measure for cardiometabolic diseases. The individual response to lifestyle interventions varies and cannot currently be predicted. Recent research has shown that Lac-Phe produced during exercise could contribute to weight loss and potentially explain differences in the effectiveness of exercise interventions in humans.
Article
Medical Laboratory Technology
Disheng Feng, Zixuan Wang, Hang Li, Xianzhe Shi, Lin Zou, Hongwei Kong, Zhiliang Xu, Chaowen Yu, Chunxiu Hu, Guowang Xu
Summary: This study developed a highly efficient method for rapid and accurate measurement of 17-alpha-hydroxyprogesterone (17-OHP) and related steroids in amount/volume-limited clinic samples, which is of great importance for the precise newborn diagnosis of congenital adrenal hyperplasia (CAH) and its subtypes in clinic.
CLINICA CHIMICA ACTA
(2023)
Article
Engineering, Environmental
Chang Wang, Huayu Fu, Jun Yang, Lei Liu, Fenghong Zhang, Chunyu Yang, Hongyuan Li, Jiamiao Chen, Qi Li, Xiaolin Wang, Yaorui Ye, Nan Sheng, Yong Guo, Jiayin Dai, Guowang Xu, Xinyu Liu, Jianshe Wang
Summary: A study found that PFECAs have inhibitory effects on cellular stress signals and lead to hepatomegaly in mice. Additionally, PFECAs also have inhibitory effects on glucocorticoid receptors, which may result in extensive suppression of stress signals. Unlike other similar substances, the next-generation replacements of legacy PFASs, PFECAs, may pose potential risks to human health.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Qingqing Wang, Miriam Hoene, Chunxiu Hu, Louise Fritsche, Robert Ahrends, Gerhard Liebisch, Kim Ekroos, Andreas Fritsche, Andreas L. Birkenfeld, Xinyu Liu, Xinjie Zhao, Qi Li, Benzhe Su, Andreas Peter, Guowang Xu, Rainer Lehmann
Summary: Reliability, robustness, and interlaboratory comparability of quantitative measurements in clinical lipidomics studies are crucial. The different ex vivo stability of lipids in blood poses a risk to data interpretation. Clear recommendations for blood sample collection are necessary. In this study, we used UHPLC-high resolution mass spectrometry to investigate the stability of 417 lipid species in EDTA whole blood under different temperature conditions and time points. Our data suggest that cooling whole blood immediately and continuously, and separating plasma within 4 hours, unless focusing on robust lipids, are recommended. Lists of lipids' ex vivo (in)stability and potential biomarkers in whole blood are provided. Overall, our results contribute to the international efforts in achieving reliable and comparable clinical lipidomics studies in the future.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Chemistry, Analytical
Xingyu Guo, Lina Zhou, Yi Wang, Feng Suo, Chuanxia Wang, Wei Zhou, Lingshan Gou, Maosheng Gu, Guowang Xu
Summary: This study developed a LC-MS-based method for neonatal metabolomics analysis of DBS and investigated the influences of blood volume and chromatographic effects on metabolite levels. The method showed good repeatability, precision, and linearity, and was applied to study metabolic disruptions of congenital hypothyroidism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Xin Lu, Peng Dou, Chao Li, Fujian Zheng, Lina Zhou, Xiaoyu Xie, Zixuan Wang, Guowang Xu
Summary: This study proposes an annotation strategy involving the derivatization of dipeptides and tripeptides via dansylation, which can choose dipeptides/ tripeptides using a small number of standards. A total of 198 dipeptides and 149 tripeptides were annotated, and the variations in serum dipeptides/tripeptides of three different liver diseases were characterized.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Neurosciences
Xiaojiao Xu, Qiu Yang, Zheyi Liu, Rong Zhang, Hang Yu, Manli Wang, Sheng Chen, Guowang Xu, Yaping Shao, Weidong Le
Summary: This study aimed to investigate the molecular changes at both metabolic and proteomic levels during the longitudinal progression of ALS and identify the most critical metabolic pathways and underlying mechanisms involved in ALS pathophysiological changes. The study found differential expressions of metabolites involved in purine metabolism, methionine cycle, and glycolysis in ALS mice, and abnormal expressions of enzymes in these metabolic pathways were also confirmed. Additionally, fatty acid metabolism, TCA cycle, arginine and proline metabolism, and folate-mediated one-carbon metabolism were significantly altered in this disease. The identified differential metabolites and proteins could complement existing data on metabolic reprogramming in ALS and provide new insights into the pathological mechanisms and therapeutic targets of ALS.
NEUROBIOLOGY OF DISEASE
(2023)
Review
Chemistry, Analytical
Di Yu, Lina Zhou, Xinyu Liu, Guowang Xu
Summary: The introduction of stable isotope labeling (SIL) technology has become a routine tool for functional metabolomics studies and allows for a deeper understanding of dynamic metabolic regulation in organisms. Mass spectrometry (MS) techniques have shown great vitality in this field by utilizing the different mass spectrometric characteristics between endogenous metabolites and their isotope-labeling forms. Recent advances in MS-based stable isotope-resolved metabolomics (SIRM) methods have expanded the breadth and depth of SIRM research by improving sample pretreatment, MS analysis, and data analysis. This review describes the recent progress of SIRM methods, summarizes their applications in metabolite identification, accurate quantification, and metabolic flux analysis, and discusses current limitations and challenges.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Environmental Sciences
Yuting Wang, Lina Zhou, Tiantian Chen, Lei You, Xianzhe Shi, Xinyu Liu, Sijia Zheng, Jie Jiang, Yuebin Ke, Guowang Xu
Summary: In this study, a 2D-LC-HRMS-based screening strategy was developed for simultaneously screening pesticides, veterinary/human drugs, and other chemical pollutants in serum. The method was validated to detect 92% and 81% of 1022 residues spiked in serum at concentrations of 50 ng/mL and 5 ng/mL, respectively. This strategy is helpful for studying the effect of exogenous exposures on human health.
ENVIRONMENTAL POLLUTION
(2023)
Article
Chemistry, Applied
Yingying Li, He Zhou, Tian Tian, Yihong Hou, Dan Chen, Jie Zhou, Shuyuan Liu, Youben Yu, Weidong Dai, Tianshan Zhou
Summary: This study investigated the effects of golden flora amount on the sensory quality, metabolites, and bioactivities of Fu brick tea. Different golden flora amounts were used to prepare the tea samples, resulting in changes in tea liquor color and astringent taste. Targeted analysis revealed decreased levels of (-)-epigallocatechin gallate, (-)-epicatechin gallate, and most amino acids with increased golden flora. Untargeted analysis identified 70 differential metabolites, where 16 compounds, including two Fuzhuanins and four EPSFs, were positively correlated with golden flora amount. Fu brick tea samples with golden flora demonstrated higher inhibitory potency on alpha-amylase and lipase. These findings provide a theoretical basis for guiding the processing of Fu brick tea based on desired sensory quality and metabolites.
Article
Chemistry, Applied
Jianjian Gao, Mengxue Zhou, Dan Chen, Jiye Xu, Zhe Wang, Jiakun Peng, Zhiyuan Lin, Shuai Yu, Zhi Lin, Weidong Dai
Summary: An affinity selection-mass spectrometry method was used to screen for α-glucosidase (AGH) inhibitors from teas, resulting in the identification of 14 galloylated polyphenols (GPs) out of 19 inhibitor candidates. Various studies suggested that GPs noncompetitively inhibit AGH activity by interacting with amino acid residues near the active site of AGH and inducing changes in AGH secondary structure. In cellular and animal experiments, representative GPs and white tea extract (WTE) showed comparable AGH inhibition effects and hypoglycemic efficacy as acarbose. These findings provide a high-efficiency approach to discover novel AGH inhibitors and explain the potential mechanism by which tea reduces diabetes risks.
Article
Biochemical Research Methods
Xiaoyu Xie, Liangqiang Chen, Tiantian Chen, Fan Yang, Zixuan Wang, Yang Hu, Jianjun Lu, Xin Lu, Qi Li, Xiuqiong Zhang, Ming Ma, Li Wang, Chunxiu Hu, Guowang Xu
Summary: This study presents a chlorine isotope labeling-assisted ultrahigh-performance liquid chromatography-high resolution mass spectrometry-based method for profiling and annotating carbonyl compounds in sauce flavored-Baijiu Daqu. A total of 487 carbonyl compounds were detected, among which 314 (64.5%) were positively or putatively identified. The method provides an effective and economical strategy for comprehensive analysis of carbonyl compounds in complex matrix samples.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Editorial Material
Chemistry, Analytical
Xinyu Liu, Xin Lu, Xianzhe Shi, Guowang Xu
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)