Article
Pharmacology & Pharmacy
Zheng-Wei Zhang, Chun-Sheng Gao, Heng Zhang, Jian Yang, Ya-Ping Wang, Li-Bin Pan, Hang Yu, Chi-Yu He, Hai-Bin Luo, Zhen-Xiong Zhao, Xin-Bo Zhou, Yu-Li Wang, Jie Fu, Pei Han, Yu-Hui Dong, Gang Wang, Song Li, Yan Wang, Jian-Dong Jiang, Wu Zhong
Summary: Morinda officinalis oligosaccharides alleviate depression by increasing 5-HTP levels in the gut microbiota.
ACTA PHARMACEUTICA SINICA B
(2022)
Review
Biotechnology & Applied Microbiology
Xin-Xin Liu, Bin Zhang, Lian-Zhong Ai
Summary: 5-hydroxytryptophan (5-HTP) plays a crucial role in regulating various physiological functions and is used in treating depression, insomnia, and other diseases. Due to inadequate natural extraction methods, increasing production efficiency through enzyme directed evolution and substrate supply pathways has become necessary to meet market demands.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Sena L. Field, Marcela G. Marrero, Lihe Liu, Francisco Penagaricano, Jimena Laporta
Summary: This study characterized the transcriptomic profile and microstructure of adipose and muscle in pre-weaned calves with increased circulating serotonin. The supplementation of 5-HTP influenced cell proliferation and metabolic pathways in both adipose and muscle tissues. This manipulation of the serotonin pathway may lead to improvements in calf growth and development.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Shih-Shin Liang, Po-Tsun Shen, Yu-Qing Liang, Yi-Wen Ke, Chieh-Wen Cheng, Yi-Reng Lin
Summary: In this study, we used isotopic formaldehyde and sodium cyanoborohydride through reductive amination to label two methyl groups on primary amine for the preparation of standards and internal standards of tryptophan and its metabolites. The derivatized reactions showed high yields and were suitable for manufacturing standards and internal standards. This method created one or two methyl groups on amine to generate different mass unit shifts, providing a useful tool for biomolecules with amine groups. Using serotonin, 5-hydroxytryptophan, and tryptophan as examples, we successfully demonstrated the application of isotopic formaldehyde for generating standards and internal standards. The derivatized method showed good linearity, with coefficients of determination ranging from 0.9938 to 0.9969, and limits of detection and quantification ranging from 1.39 to 15.36 ng/mL.
Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Chemistry, Physical
BingBing Wang, Shuai Liu, HaiDe Wang, JianZhong Xu, WeiGuo Zhang
Summary: In this study, a new in vitro multi-enzyme cascade system (MECCS) was developed for the efficient production of 5-HTP. The mutant M1 showed excellent enzymatic performance, with a 2.3-fold increase in catalytic activity. Under optimized conditions, the MECCS achieved a conversion rate of 73.8% for the conversion of L-tryptophan to 5-HTP.
MOLECULAR CATALYSIS
(2023)
Article
Physiology
Kristoffer Anton Lundgren, Hanne Lovlie
Summary: Our production animals typically have poor welfare, and reducing fearfulness through dietary manipulation of serotonin precursor has shown promising results in reducing negative affective state in hens.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Chemistry, Analytical
Albert Perez-Alcaraz, Francesc Borrull, Marta Calull, Carme Aguilar
Summary: The consumption of synthetic cathinones has been increasing in recent years due to its affordability and accessibility as an alternative to amphetamines. Developing procedures capable of detecting and monitoring their consumption is crucial, and urine has been widely used for this purpose as it is non-invasive and provides larger sample volumes than other biological matrices. Chromatographic techniques are the most reported approach for determining cathinones in urine samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Feifei Song, Tao Gu, Lin Zhang, Jiaxing Zhang, Shengping You, Wei Qi, Rongxin Su
Summary: This study improved the catalytic activity of L-Trp-TPH1-BH4 by rational design and obtained the S337A/F318Y beneficial mutant, which increased the yield of 5-HTP and produced 0.91 g/L of 5-HTP, offering the prospect of green production of 5-HTP.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Michele Spinelli, Alessandra Biancolillo, Gennaro Battaglia, Martina Foschi, Angela Amoresano, Maria Anna Maggi
Summary: This study aimed to evaluate if saffron samples produced in different years and origins have significantly different crocin compositions. By analyzing 120 samples harvested in four different Italian areas using HPLC-ESI-MS/MS, the relevance of origin and annuity in affecting crocin composition was confirmed. SIMCA models were created for each origin and tested using mixtures of saffron from different areas, providing satisfying results.
Article
Neurosciences
Hannah B. Weinberg-Wolf, Nick Fagan, Olga Dal Monte, Steve W. C. Chang
Summary: The study found that increasing central concentrations of serotonin with the direct precursor 5-HTP impairs the ability of rhesus macaques to flexibly orient and inhibit, particularly inhibiting orienting to face images. The effects of 5-HTP on performance were correlated with physiological changes such as pupil constriction, increased time to initiate trials, and increased reaction time, suggesting a downregulation of arousal and motivational states.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Biochemical Research Methods
Emanuele Di Carlo, Silvia Santagata, Luca Sauro, Manuela Tolve, Filippo Manti, Vincenzo Leuzzi, Antonio Angeloni, Claudia Carducci
Summary: The study developed and validated a fast and specific diagnostic tool for detecting 5-HTP and 3OMD, improving the specificity and sensitivity for the biochemical diagnosis of AADC deficiency.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Environmental Sciences
A. Ruth Godfrey, Jonathan Dunscombe, Anthony Gravell, Ann Hunter, Mark P. Barrow, Geertje van Keulen, Claire Desbrow, Rachel Townsend
Summary: The study demonstrates that the modified QuEChERS sample preparation method is a viable option for efficient and cost-effective monitoring of environmental pollutants. The method offers reduced extraction times, good reproducibility, and the ability to differentiate between different samples. It also identifies new potential pollutant candidates.
Article
Chemistry, Applied
Sung-Tong Chin, Gerhard Hoerlendsberger, Kok Wai Wong, Sirui Li, Sze How Bong, Luke Whiley, Julien Wist, Reika Masuda, Johan Greeff, Elaine Holmes, Jeremy K. Nicholson, Ruey Leng Loo
Summary: In this study, a one-step isopropanol method and a two-step methyl-tert-butyl ether method were compared for extracting lipid species in chicken egg yolks before LC-MS/MS analysis. The isopropanol method showed high reproducibility in extracting 937 lipid species from 20 major lipid subclasses. Machine learning techniques successfully differentiated cage, barn, and free-range eggs with high accuracy. The lipid profiles of these eggs were found to be characteristic and could be used for verifying the provenance of conventional chicken eggs.
Article
Veterinary Sciences
Diana Victoria Arbona, Larry D. Cowgill, Saya Press, Cedric Dufayet, Stephanie Istvan
Summary: This article describes a case of 5-hydroxytryptophan (5-HTP) toxicity in a dog successfully treated with haemodialysis. The dog presented to the emergency department approximately 4-5 hours after ingesting a human supplement containing 200 mg of 5-HTP, showing progressive neurologic abnormalities consistent with serotonin syndrome. High-efficiency haemodialysis was initiated and the dog showed continued clinical improvement throughout the 5-hour treatment, with complete resolution of clinical signs within 12 hours.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Food Science & Technology
Noelia Rosales-Conrado, Maria Eugenia de Leon-Gonzalez, Luis Maria Polo-Diez
FOOD ANALYTICAL METHODS
(2015)
Article
Chemistry, Analytical
Maria Eugenia Leon-Gonzalez, Noelia Rosales-Conrado
ENVIRONMENTAL CHEMISTRY
(2016)
Article
Chemistry, Multidisciplinary
Luis Vicente Perez-Arribas, Maria Eugenia Leon-Gonzalez, Noelia Rosales-Conrado
JOURNAL OF CHEMICAL EDUCATION
(2017)
Article
Biochemical Research Methods
Noelia Rosales-Conrado, Margherita Dell'Aica, Maria Eugenia de Leon-Gonzalez, Luis Vicente Perez-Arribas, Luis Maria Polo-Diez
BIOMEDICAL CHROMATOGRAPHY
(2013)
Review
Biochemical Research Methods
Maria Eugenia Leon-Gonzalez, Noelia Rosales-Conrado, Luis Vicente Perez-Arribas, Vanesa Guillen-Casla
BIOMEDICAL CHROMATOGRAPHY
(2014)
Article
Food Science & Technology
Noelia Rosales-Conrado, Vanesa Guillen-Casla, Luis Vicente Perez-Arribas, Maria Eugenia de Leon-Gonzalez, Luis Maria Polo-Diez
FOOD ANALYTICAL METHODS
(2013)
Article
Chemistry, Analytical
David Nunez-Alonso, Luis Vicente Perez-Arribas, Sadia Manzoor, Jorge O. Caceres
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
(2019)
Article
Environmental Sciences
J. O. Caceres, D. Sanz-Mangas, S. Manzoor, L. V. Perez-Arribas, J. Anzano
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Environmental Sciences
C. Marina-Montes, L. Perez-Arribas, M. Escudero, J. Anzano, J. O. Caceres
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Environmental Sciences
Cesar Marina-Montes, Luis Vicente Perez-Arribas, Jesus Anzano, Jorge O. Caceres
Article
Spectroscopy
Jesus M. Anzano, Andres Cruz-Conesa, Roberto J. Lasheras, Cesar Marina-Montes, Luis Vicente Perez-Arribas, Jorge O. Caceres, Abrahan I. Velasquez, Vincenzo Palleschi
Summary: Laser-induced breakdown spectroscopy (LIBS) is a quick technique for analyzing various samples, including geological samples like soils, without the need for standard references. Calibration Free (CF) methodology can be used for multielemental quantitative analysis without standards, by studying the physical parameters of the plasma created by laser-sample interaction. By optimizing instrumental parameters, choosing appropriate spectral lines, and using signal to noise ratio (SNR) evaluation, high-quality spectral results can be obtained for complex samples like Antarctic soils.
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Cesar Marina-Montes, Vincent Motto-Ros, Luis Vicente Perez-Arribas, Jesus Anzano, Maria Millan-Martinez, Jorge O. Caceres
Summary: The article presents an innovative method based on micro laser-induced breakdown spectroscopy for characterizing PM collected in Antarctica, which can quickly obtain a global visualization of elemental PM composition under atmospheric pressure. This method can map the localization of pollutants in filters at high spatial resolution and speed.
ANALYTICA CHIMICA ACTA
(2021)
Article
Spectroscopy
Cesar Marina-Montes, Luis Perez-Arribas, Jesus Anzano, Silvia Fdez-Ortiz de Vallejuelo, Julene Aramendia, Leticia Gomez-Nubla, Alberto de Diego, Juan Manuel Madariaga, Jorge O. Caceres
Summary: The non-destructive spectroscopic characterization of airborne particulate matter in the Antarctic region revealed the complex elemental and molecular composition of atmospheric aerosols at the particle level. Raman spectroscopy and Scanning Electron Microscopy with Energy-Dispersive X-ray Spectroscopy techniques identified a variety of organic compounds, black carbon, exotic minerals, and natural PM in the samples collected from the surroundings of the Spanish Antarctic Research Station Gabriel de Castilla. The presence of microplastic fibers in the Antarctic air for the first time suggests the influence of anthropogenic activities even in pristine and remote regions.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
J. J. Camacho, J. Vrabel, S. Manzoor, L. V. Perez-Arribas, D. Diaz, J. O. Caceres
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2019)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
