Article
Biochemical Research Methods
Sisem Ektirici, Ilgim Gokturk, Fatma Yilmaz, Adil Denizli
Summary: Boronic acids are important ligands for selectively recognizing and enriching compounds containing cis-diol groups like nucleosides. The [POSS-MAH-PBA] boronate affinity monolithic column exhibited higher selectivity for adenosine compared to [POSS-MAH] column, making it a promising adsorbent for selective adsorption of cis-diol containing compounds in biological fluids. Methacryl Polyhedral Oligomeric Silsesquioxanes (POSS-MA) provided the advantage of high surface area and stable 3-dimensional architectures for the adsorbent.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Review
Chemistry, Analytical
Josiane F. da Silva, Clara M. G. Lima, Debora L. da Silva, Ivonea S. do Nascimento, Sarah de O. Rodrigues, Leticia A. Goncalves, Renata F. Santana, Waseem Khalid, Silvani Verruck, Talha Bin Emran, Irwin R. A. de Menezes, Henrique D. M. Coutinho, Mayeen U. Khandaker, Mohammad R. I. Faruque, Rafael da C. I. Fontan
Summary: Growing interest in medical, engineering, biochemical, and biological fields has led to continuous research and development of polymeric monolithic structures with densely interconnected pores for purifying bio compounds. Cryogels, generated by freezing a reactive polymerization mixture, offer versatility and cost-effectiveness as macroporous, polymeric, monolithic adsorbents. The conversion of cryogels into affinity adsorbents, particularly using epoxy radicals as supports, provides a promising alternative for biomolecule purification.
Article
Biochemical Research Methods
Guizhen Liu, Yanan Du, Tao Fu, Ying Han, Lifeng Pan, Jingwu Kang
Summary: The study presented a method for profiling protein-protein interactions using cm-IMAC columns in combination with label-free quantitative proteomics, successfully exploring protein-protein interactions and identifying new interactors of Bcl-X L.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Zhang-Na Qin, Xi Chen, Qiong-Wei Yu, Jun Ding, Yu-Qi Feng
Summary: Protein phosphorylation is crucial for biological functions, and in this study, a method for phosphopeptide enrichment using an arsenate functionalized monolithic column coupled with mass spectrometry analysis was developed successfully. The method exhibited high selectivity and satisfactory performance in analyzing phosphopeptides from complex biological samples such as human serum and rat brain proteins.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Jing Chen, Liangwen Hao, Jiaqi Hu, Kang Zhu, Yu Li, Sicheng Xiong, Xiaolin Huang, Yonghua Xiong, Ben Zhong Tang
Summary: A universal boronate-affinity crosslinking-amplified DLS immunoassay was developed for point-of-care glycoprotein detection, achieving ultrasensitive and rapid quantitative assay within 15 minutes. This approach combines antibody-coated magnetic nanoparticles for target capture and DLS signal transduction with phenylboronic acid-based boronate-affinity materials as crosslinking amplifiers, making it a promising strategy for extending DLS technique to detect glycoproteins even in challenging environments.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Engineering, Environmental
Rui Gomes Nascimento, Marjorie Castro Pinto Porfirio, Annie Nolasco Alves, Priscilla Amaral Nascimento, Leandro Soares Santos, Cristiane Martins Veloso, Renata Cristina Ferreira Bonomo, Rafael da Costa Ilheu Fontan
Summary: Polymeric monolithic structures functionalized with IDA + Cu2+ were prepared and evaluated for protein adsorption. The adsorption equilibrium of bovine serum albumin on polyacrylamide cryogel was analyzed using Langmuir model and the enthalpy and entropy of adsorption were calculated. Microscopy analysis confirmed the porosity of the cryogels. The results showed that the columns produced had high swelling capacity, degree of expansion, porosity, and high permeability, indicating resistance to flow. The adsorption process was favored by increased temperature and occurred spontaneously for all temperatures studied. The stability of the column to reuse cycles was an interesting feature of this study.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Xue Zhang, Qin Wei, Xin Meng, Lili Zhao, Zhaosheng Liu, Yanping Huang
Summary: A new strategy utilizing a dendrimer-like structure of boron materials based on octavinyl-polyhedral oligomeric silsesquioxanes (Ov-POSS) was suggested to improve boronate avidity with good accessibility of sites. The resulting Ov-POSS cross-linked boron monolith showed significantly stronger affinity for glycoproteins compared to the Ov-POSS-free monolith. The developed microfluidic platform, which integrated multiple functions, demonstrated high efficiency and high-throughput analysis for post-translational modification proteomics.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Chemical
Wei Gao, Feng Zhang, Sen Zhang, Jia-yuan Li, Hong-zhen Lian
Summary: High selective enrichment of low abundance phosphopeptides from complex biological samples is essential for in-depth phosphoproteomics. A magnetic material called mMOF-FBP-Ti4+, fabricated by grafting UiO-66-NH2 metal-organic framework onto the surface of Fe3O4 and immobilizing Ti(IV) with fructose 1,6-bisphosphate, exhibited favorable properties for phosphopeptide enrichment. The material demonstrated strong affinities through metal oxide affinity chromatography and immobilized metal affinity chromatography, as well as improved hydrophilicity for enhanced hydrophilic chromatography interaction. The mMOF-FBP-Ti4+ also yielded low detection limit, high selectivity, good reusability, and high postenrichment recovery towards phosphopeptides, making it a promising tool for efficient enrichment in complex biological samples.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Fa-Yi Wei, Hong-Wei Zheng, Jiao-Jiao Tian, Hong-Ying Liu, Ying-Xin Wei, Lu Yang, Chang-Yun Wang, Chang-Hu Xue
Summary: This study developed a hierarchical macroporous agarose monolith with multiple boronate affinity sites for selective separation of neomycin residues in food samples. The composite monolith showed high binding ability and the binding capacity could be controlled by adjusting the pH and adding monosaccharides. The monolith successfully purified neomycin from spiked model aquatic products, indicating its great potential in the separation of neomycin from complex matrices.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemical Research Methods
Andrea Gottardini, Vincent Dugas, Claire Demesmay
Summary: Affinity chromatography is a powerful technique for identifying and quantifying weak ligand-protein interactions. A novel approach of protein immobilization based on streptavidin-biotin interaction was presented in this study to improve bulk density of active protein, expanding the affinity range of the technique to the millimolar range.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Multidisciplinary
Huimin Chu, Haoyang Zheng, Aizhu Miao, Chunhui Deng, Nianrong Sun
Summary: This study used hybrid metal organic frameworks (HMOFs) to investigate the region-resolved phosphoproteome of the human lens, identifying phosphosites and phosphoproteins in the capsule, cortex, and nucleus. This provided a relatively extensive distribution map of phosphorylation in the human lens for the first time.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Bailin Guo, Yukui Tong, Baodong Sun, Baoyue Zhang, Xue Chen, Sheng Bi, Miaomiao Tian
Summary: Metal oxide-based macroporous ordered double affinity molecularly imprinted polymers (D-MIPs) were developed as solid phase extraction (SPE) adsorbents for specific identification of ovalbumin (OVA) under physiological pH conditions. The adsorbent showed high adsorption capacity and selectivity towards OVA, making it a promising method for enriching OVA and other cis-diol-containing analytes in complex biological samples.
Article
Chemistry, Analytical
Hongwei Wang, Ruizhi Tang, Shicong Jia, Shujuan Ma, Bolin Gong, Junjie Ou
Summary: This paper introduces a novel monodisperse MAR microsphere (Ti4+-Brush@MAR) prepared and modified via surface-initiated atom transfer radical polymerization (SI-ATRP), showing high enrichment specificity for phosphopeptides in beta-casein or milk digest samples.
Article
Biochemical Research Methods
Pandeng Miao, Liu Zhang, Jian Zhang, Mingxuan Ma, Yingxiang Du, Jie Gan, Jiangxia Yang
Summary: Metal-organic frameworks (MOFs) are microporous hybrid materials formed by coordination of metal cations with organic ligands. In this study, MOF-5 was grown on a polymer support and bonded with pepsin for enantioseparation of chiral drugs.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemical Research Methods
Huan Huang, Qiong Zheng, Yanting He, Chao Zhong, Wenchang Tian, Shasha Zhang, Juan Lin, Zian Lin
Summary: This study introduces a new bifunctional polymer monolithic column for tunable and specific enrichment of glycoproteins and phosphoproteins. The poly (EDMA-co-VPBA-co-VPA) monolith exhibited superior performance in selective separation of small molecules and biomacromolecules containing cis-diol/phosphate groups, with higher binding capacity towards glycoproteins and phosphoproteins compared to non-glycosylated/phosphorylated proteins. The successful application in specific enrichment of glycopeptides/phosphopeptides from complex samples highlights the practicality of this approach in post-translational modification-proteomics development.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)