Article
Chemistry, Analytical
Yao Lu, Zhihong Yan, Genlin Sun
Summary: A stationary phase based on sub-2 μm ground silica monolith particles was prepared and applied for peptide separation in micro-columns. The optimized flow rate achieved an average of 9800 plates/column (5.10 μm plate height) for the stationary phase in glass-lined stainless steel micro-columns, demonstrating good repeatability.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Polymer Science
Ashraf Ali, Sarah Alharthi, Nora Hamad Al-Shaalan, Eman Y. Santali
Summary: Efficient separation with good resolution is crucial in the pharmaceutical industry, especially for complex samples like peptides and proteins. In this study, silica monolith particles were derivatized and packed into narrow-bore columns, achieving high efficiency and resolution for the separation of peptides and proteins. The developed method allows for fast analysis and detection of these compounds with previously unreported efficiency and resolution.
Article
Chemistry, Analytical
Thomas Themelis, Jelle De Vos, Sebastiaan Eeltink
Summary: This article discusses the design aspects of microfluidic chips for spatial three-dimensional chromatography and establishes the corresponding kinetic performance limits using a Pareto-optimality approach. The advantages and disadvantages of integrating different separation mechanisms are discussed, and novel equations are proposed to determine the peak capacity and account for sample modulation. A microfluidic spatial prototype chip has been created with the potential to achieve a high peak capacity within a short analysis time.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Vahid Ganjalizadeh, Gopikrishnan G. Meena, Thomas A. Wall, Matthew A. Stott, Aaron R. Hawkins, Holger Schmidt
Summary: This article introduces a signal analysis technique based on a massively parallel continuous wavelet transform algorithm, which can accurately and quickly handle weak signals and background noise. Through experimental demonstrations, the superiority of this method is proved, and the performance improvement in detecting bacterial DNA is shown.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Faiz Ali, Won Jo Cheong, Aamra Rafique, Zeid A. AlOthman, Maria Sadia, Mian Muhammad
Summary: A particle-based stationary phase was prepared for separating synthetic peptides and a mixture of tryptic digest of cytochrome C in liquid chromatography. The stationary phase showed very high separation efficiency for commercial peptides and was able to resolve multiple peptide components from a complex mixture.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Engineering, Environmental
Hao Ai, Siyu Jian, Yuan Yao, Kaifeng Du
Summary: A novel aligned porous Chitosan/Ti3C2 MXenes monolith was successfully synthesized through a facile, versatile, and reproducible unidirectional freeze-casting method. The Ti3C2 MXenes accumulation guided the formation of an aligned structure, resulting in the formation of aligned macropores through a subsequent freeze-drying process. Various physical characterizations determined the distribution of aligned macropores, micropores, and specific surface area. Batch adsorptive experiments showed significantly enhanced adsorptive capacities to bovine serum albumin and bovine hemoglobin. The aligned macropores were found to facilitate the film mass transfer factor in a separation process, driving the global mass transfer factor.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Food Science & Technology
Guanhua Hu, Debao Wang, Lina Sun, Rina Su, Mirco Corazzin, Xueying Sun, Lu Dou, Min Zhang, Lihua Zhao, Lin Su, Ye Jin
Summary: Using a dual-enzyme system, sheep bone protein was hydrolyzed to isolate a novel peptide with strong calcium-chelating ability. The fluorescence, circular dichroism, and infrared spectroscopy results showed changes in protein structure upon calcium binding. Two calcium-binding peptides were identified in the sheep bone protein hydrolysate.
Article
Chemistry, Analytical
Jonas Neumann, Sebastian Schmidtsdorff, Alexander H. Schmidt, Maria K. Parr
Summary: Supercritical fluid chromatography has emerged as an alternative analytical tool for the analysis of biopharmaceuticals. In this study, a systematic approach was developed for fingerprinting tyrothricin, a complex therapeutic peptide, using supercritical fluid chromatography. The final method using a diol stationary phase, carbon dioxide, and a modifier consisting of methanol/water/methanesulfonic acid showed orthogonality and superior resolution compared to other methods. Isomeric peptide compounds coeluting in reversed-phase liquid chromatography were successfully resolved.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ashraf Ali, Sarah Alharthi, Bashir Ahmad, Alia Naz, Idrees Khan, Fazal Mabood
Summary: Chemically modified silica monolith particles were prepared for the adsorptive removal of lead (II) ions from aqueous medium. The adsorbent showed an adsorption capacity of 792 mg/g for Pb(II) ions, with a 98% adsorption rate under optimal conditions of pH 5, 0.4 g/L adsorbent dose, 500 mg/L Pb(II) ion concentration, and 1 hour adsorption time. The adsorption data fitted well with Freundlich and Langmuir isotherm models, and the adsorption process followed a pseudo second-order kinetic model, suggesting spontaneous and exothermic adsorption.
Article
Biochemical Research Methods
Daniele Peterle, David DePice, Thomas E. Wales, John R. Engen
Summary: In reversed-phase peptide separation for HDX-MS, the slowest back exchange is achieved under low pH and low temperature quench conditions. To maximize deuterium retention, separation must be done quickly. However, the low temperature complicates the separation and results in decreased separation quality and increased chromatographic backpressure. Various strategies can be employed to improve the separation efficiency under HDX-MS quench conditions, such as using longer gradients, smaller particles, alternative separation mechanisms, or higher flow rates.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Xiaofei Wang, Jue Zhu, Chenyuhu Yang, Fei Qin, Bo Zhang
Summary: A segmented microfluidic method was developed for preparing packed capillary columns with liquid segments as reservoirs for carrying and delivering packing particles, resulting in ultralong columns with excellent separation efficiencies. The microfluidically packed columns demonstrated higher peak capacity production efficiency in the high-resolution region, showing significantly improved protein identification capability in proteomic analysis compared to a commercial nanoLC column.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Samuel T. Dada, Maarten C. Hardenberg, Zenon Toprakcioglu, Lena K. Mrugalla, Mariana P. Cali, Mollie O. McKeon, Ewa Klimont, Thomas C. T. Michaels, Tuomas P. J. Knowles, Michele Vendruscolo
Summary: Using fluorescence-based assays, we elucidate the microscopic mechanism of α-synuclein aggregation within liquid condensates. At physiological pH (7.4), this process begins with spontaneous primary nucleation followed by rapid aggregate-dependent proliferation. Accurate quantification of the kinetic rate constants for the appearance and proliferation of α-synuclein aggregates reveals the detailed mechanism of aggregation within condensates.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Physical
Yangtao Liu, Xiangtao Gong, Chinmoy Podder, Fan Wang, Zeyuan Li, Jianzhao Liu, Jinzhao Fu, Xiaotu Ma, Panawan Vanaphuti, Rui Wang, Andrew Hitt, Yavuz Savsatli, Zhenzhen Yang, Mingyuan Ge, Wah-Keat Lee, Bryan Yonemoto, Ming Tang, Heng Pan, Yan Wang
Summary: In response to the demand for LIBs, a solvent-free manufacturing technology is demonstrated to avoid toxic organic solvents and improve electrode structures. The dry-printed (DP) electrodes have lower tortuosity, allowing for better rate performance and higher capacity retention compared to slurry cast (SL) electrodes. The coating layer on active materials in DP cells prevents side reactions and prolongs cycle life. This roll-to-roll manufacturing process has immense potential for scalable and efficient battery production.
Article
Thermodynamics
Chuanping Lin, Jun Xu, Mingjie Shi, Xuesong Mei
Summary: This paper proposes a battery state of health estimation method based on constant current charging time, which can accurately and quickly estimate the health status of the battery. Compared with traditional methods, this method has higher prediction accuracy, requires less data, and has shorter training and prediction time.
Article
Biochemistry & Molecular Biology
Angelita Rebollo, Eric Savier, Pierre Tuffery
Summary: PEPscan is an effective method for identifying interfering peptides, which can interfere with protein-protein interactions. It is able to rapidly and accurately identify specific interfering peptides, but further validation is needed regarding their location at the protein-protein interface.
Review
Biochemical Research Methods
Ann Lin, Froydis Sved Skottvoll, Simon Rayner, Stig Pedersen-Bjergaard, Gareth Sullivan, Stefan Krauss, Steven Ray Wilson, Sean Harrison
Review
Endocrinology & Metabolism
Shadab Abadpour, Aleksandra Aizenshtadt, Petter Angell Olsen, Kayoko Shoji, Steven Ray Wilson, Stefan Krauss, Hanne Scholz
CURRENT DIABETES REPORTS
(2020)
Article
Toxicology
Marianne Skov-Skov Bergh, Inger Lise Bogen, Elisabeth Nerem, Ariane Wohlfarth, Steven Ray Wilson, Ase Marit Leere Oiestad
Summary: This study aimed to discover and elucidate the major metabolites of three novel fentanyl analogues, providing important markers for confirming the intake of these compounds in forensic cases.
FORENSIC TOXICOLOGY
(2021)
Article
Biochemical Research Methods
Ahmad Tsjokajev, Hanne Roberg-Larsen, Steven Ray Wilson, Anne-Berit Dyve Lingelem, Tore Skotland, Kirsten Sandvig, Elsa Lundanes
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2020)
Review
Chemistry, Analytical
Hanne Roberg-Larsen, Steven Ray Wilson, Elsa Lundanes
Summary: This review focuses on the application of nLC-MS in sample preparation, particularly through online upfront devices and online reaction systems to improve the sensitivity and robustness of samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Malgorzata Zawadzka, Beatrix Racz, Dario Ambrosini, Carl Henrik Goerbitz, Jens Preben Morth, Alistair L. Wilkins, Anja Osteby, Katja Benedikte Presto Elgstoen, Elsa Lundanes, Frode Rise, Amund Ringvold, Steven Ray Wilson
Summary: Research discovered an unknown UV-absorbing compound in the aqueous humor of geese, yet the protective compound X was not found. It is possible that the level of UV protective compound in goose aqueous humor is lower than previously identified compounds, or that it may have degraded during the isolation and chromatographic purification processes used.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Froydis Sved Skottvoll, Frederik Andre Hansen, Sean Harrison, Ida Sneis Boger, Ago Mrsa, Magnus Saed Restan, Matthias Stein, Elsa Lundanes, Stig Pedersen-Bjergaard, Aleksandra Aizenshtadt, Stefan Krauss, Gareth Sullivan, Inger Lise Bogen, Steven Ray Wilson
Summary: Liver organoids can be effectively analyzed using electromembrane extraction (EME) for precise drug development and toxicity screening. Multiwell EME technology allows for simple and repeatable monitoring of drug metabolism kinetics, and is compatible with chromatographic separation methods. Liver organoids and EME are well-suited for mass spectrometry (MS)-based measurements.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Hanne Roberg-Larsen, Elsa Lundanes, Tuula A. Nyman, Frode S. Berven, Steven Ray Wilson
Summary: Single-cell analysis provides in-depth insights into diseases and diagnostics, with liquid chromatography playing a crucial role. The development of novel sample preparation techniques and improvements in mass spectrometry are enhancing the promise of single-cell analysis. However, technical challenges and the need for higher throughput and robustness may lead to the reinvention of alternative nano LC column formats.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Hanne Bendiksen Skogvold, Elise Mork Sandas, Anja Osteby, Camilla Lokken, Helge Rootwelt, Per Ola Ronning, Steven Ray Wilson, Katja Benedikte Presto Elgstoen
Summary: A single LC-MS method was developed for DBS metabolite analysis in clinical applications such as newborn screening, allowing for simultaneous analysis of a wide range of metabolites. The method utilized a diphenyl column, a multi-linear solvent gradient, and tailored MS settings to enhance sensitivity and reproducibility for diverse metabolites. The performance of the method was suitable for both untargeted and targeted approaches in clinically relevant experiments.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Chemistry, Analytical
Christine Olsen, Elisa Wiborg, Elsa Lundanes, Shadab Abadpour, Hanne Scholz, Steven Ray Wilson
Summary: The research focuses on investigating tools for coupling pancreas-on-a-chip systems with mass spectrometry. By using an inter-chain cleavage reactor and nano liquid chromatography-mass spectrometry system, the researchers achieved on-line cleavage of insulin's disulfide bonds and obtained robust fragmentation mass spectra. The use of a silica monolith in the nano liquid chromatography format improved chromatographic performance compared to particle-packed columns.
SEPARATION SCIENCE PLUS
(2022)
Article
Chemistry, Analytical
Stian Kogler, Aleksandra Aizenshtadt, Sean Harrison, Froydis Sved Skottvoll, Henriette Engen Berg, Shadab Abadpour, Hanne Scholz, Gareth Sullivan, Bernd Thiede, Elsa Lundanes, Inger Lise Bogen, Stefan Krauss, Hanne Roberg-Larsen, Steven Ray Wilson
Summary: Organoids, laboratory-grown organ models developed from stem cells, have been successfully coupled with liquid chromatography-mass spectrometry (LC-MS) to enable continuous tracking of drug metabolism.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Hanne Bendiksen Skogvold, Helge Rootwelt, Leon Reubsaet, Katja Benedikte Presto Elgstoen, Steven Ray Wilson
Summary: Dried blood spot samples offer a simple and safe option for diagnostics, with liquid chromatography-mass spectrometry being a versatile measurement tool. These samples provide valuable information for metabolomics, xenobiotic analysis, and proteomics. Targeted analysis of small molecules is the main application, but there are emerging applications in untargeted metabolomics and proteomics. Applications range from newborn screening to disease diagnostics and monitoring, as well as research into the effects of diet, exercise, xenobiotics, and doping. A variety of dried blood spot products and liquid chromatography-mass spectrometry methods are available, including novel approaches like on-paper sample preparation.
JOURNAL OF SEPARATION SCIENCE
(2023)
Review
Chemistry, Analytical
Stian Kogler, Kristina Saeterdal Komurcu, Christine Olsen, Jun-ya Shoji, Froydis Sved Skottvoll, Stefan Krauss, Steven Ray Wilson, Hanne Roberg-Larsen
Summary: Organoids and organ-on-a-chip devices are important tools for studying various aspects of organs. This review focuses on the application of mass spectrometry in studying organoids and organ-on-a-chip-derived material. It discusses the use of liquid chromatography-mass spectrometry (LC-MS) for proteomics, metabolomics/lipidomics, and hormones, as well as mass spectrometry imaging (MSI) of organoids. The analysis has provided insights into the development and disease of organs, potentially replacing or complementing animal models.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Michal Gregus, Alexander R. Ivanov, Steven Ray Wilson
Summary: Ultralow flow LC utilizes ultra-narrow bore columns and flow rates ranging from pL/min to low nL/min. It provides high sensitivity and resolution, making it suitable for analyzing scarce biological samples. Recent advancements in dedicated instrumentation, technical approaches, and higher throughput have facilitated the application of ultralow flow LC to the analysis of amount-limited samples. This review discusses the types of ultralow flow LC technology, highlights representative applications in bioanalysis, and explores future developments.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Medicine, Research & Experimental
Froydis Sved Skottvoll, Henriette Engen Berg, Kamilla Bjorseth, Kaja Lund, Norbert Roos, Sara Bekhradnia, Bernd Thiede, Cecilie Sandberg, Einar Osland Vik-Mo, Hanne Roberg-Larsen, Bo Nystrom, Elsa Lundanes, Steven Ray Wilson
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
