Article
Biochemical Research Methods
Ian D. Wilson, Colin F. Poole
Summary: Planar chromatography, in the form of thin-layer or high-performance thin-layer chromatography (TLC, HPTLC), remains a powerful and widely used separation technique. It is unparalleled as a simple and rapid qualitative method for mixture analysis, or for identifying bioactive components in mixtures. TLC/HPTLC also allows for the preservation of the separation, enabling further research on components of interest separated in both time and space from the original analysis. The current practice and future developments of planar chromatography are reviewed.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Ashis Kumar Goswami, Neelutpal Gogoi, Hemanta Kumar Sharma
Summary: In this study, a high-performance thin-layer chromatography-based densitometric method was developed for identification, quantification, and stability study of linalool. The developed method was found to be precise, accurate, and reproducible, and can be used in the analysis and quality control of herbal materials and volatile oils containing linalool.
JOURNAL OF CHROMATOGRAPHIC SCIENCE
(2023)
Article
Plant Sciences
Mohammed H. Alqarni, Faiyaz Shakeel, Tariq M. Aljarba, Maged S. Abdel-Kader, Hala H. Zaatout, Sultan Alshehri, Prawez Alam
Summary: A greener HPTLC method was developed and validated for the analysis of CLH. This method showed better accuracy and precision compared to traditional methods. It demonstrated superior performance in commercial formulations and plant extracts.
Article
Chemistry, Analytical
Ahmed I. Foudah, Faiyaz Shakeel, Mohammed H. Alqarni, Prawez Alam
Summary: A rapid and sensitive stability-indicating green reversed-phase high-performance thin-layer chromatography (SIG-RP-HPTLC) method was established and validated for the detection of flibanserin (FBN) in commercial products, showing superiority over the stability-indicating green normal-phase high-performance thin-layer chromatography (SI-G-NP-HPTLC) method. The greenness of both methods was evaluated using AGREE software, with eco-scale values suggesting excellent greenness for both techniques.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemical Research Methods
Ahmed I. Foudah, Faiyaz Shakeel, Mohammed H. Alqarni, Samir A. Ross, Mohammad A. Salkini, Prawez Alam
Summary: The study developed a highly sensitive, rapid, and eco-friendly HPTLC densitometry technique for determining TQ in various plant extracts, commercial products, and found that TQ content was highest in the Saudi Arabian extract. NP/RP densitometry were found suitable for the pharmaceutical assay of TQ with excellent greenness profile.
PHYTOCHEMICAL ANALYSIS
(2022)
Article
Engineering, Chemical
Stanislava Ivanova, Velislava Todorova, Stanislav Dyankov, Kalin Ivanov
Summary: In this study, a simple and rapid HPTLC method was developed for the detection of meloxicam and piroxicam. The method showed high sensitivity and could detect the compounds at very low concentrations. It offers a simple, cost-effective, and eco-friendly approach for research or routine quality control purposes.
Article
Chemistry, Analytical
Mohammed H. Alqarni, Faiyaz Shakeel, Ahmed Foudah, Tariq M. Aljarba, Aftab Alam, Sultan Alshehri, Prawez Alam
Summary: The study aimed to design and validate a greener HPTLC method for the estimation of VD3, which provided better accuracy and precision in VD3 analysis in commercial pharmaceutical products and demonstrated superior greenness profile compared to traditional methods.
Article
Engineering, Chemical
Prawez Alam, Faiyaz Shakeel, Wael A. Mahdi, Ahmed Foudah, Mohammed H. Alqarni, Tariq M. Aljarba, Sultan Alshehri, Mohammed M. Ghoneim
Summary: This study developed and validated a sensitive and greener HPTLC method for the estimation of gefitinib. The greener method showed higher sensitivity, accuracy, precision, and robustness compared to the routine method. Both methods were able to detect gefitinib in the presence of degradation products, indicating their stability-indicating property.
Article
Chemistry, Analytical
Ripandeep Kaur, O. P. Katare, Guneet Singh, Vishav Mohan, Sumant Saini, Bhupinder Singh
Summary: The study developed a rapid and cost-effective analysis method for the treatment drugs artemether and lumefantrine for cerebral malaria, investigated and optimized the sensitivity and assay of the drugs through HPTLC, and confirmed the accuracy and robustness of the method.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Prawez Alam, Faiyaz Shakeel, Abuzer Ali, Mohammed H. Alqarni, Ahmed I. Foudah, Tariq M. Aljarba, Faisal K. Alkholifi, Sultan Alshehri, Mohammed M. Ghoneim, Amena Ali
Summary: A rapid, sensitive, and greener reversed-phase HPTLC approach was developed for the simultaneous determination of caffeine and paracetamol in commercial formulations. This approach showed superior sensitivity, accuracy, precision, and robustness compared to the greener normal-phase HPTLC technique.
Article
Engineering, Mechanical
Luis G. Crespo, Sean P. Kenny
Summary: This study evaluates responses to the NASA Langley Challenge on Optimization under Uncertainty, examining how participants quantified uncertainty in computational model predictions and proposed reliability-based design solutions within the identified uncertainty range. The results show that through a synthetic validation framework, designs can be assessed and compared based on reliability requirements imposed by additional data.
MECHANICAL SYSTEMS AND SIGNAL PROCESSING
(2022)
Article
Biochemical Research Methods
Agnes M. Moricz, Peter G. Ott
Summary: An antibacterial component screening of Prunus armeniaca leaf extract was conducted using HPTLC-direct bioautography and HPTLC-ESI-MS techniques. Triterpenoids and fatty acids were identified as the bioactive compounds. Ursolic acid, oleanolic acid, betulinic acid, corosolic acid, and maslinic acid were discovered for the first time as antibacterial components of P. armeniaca leaves, and their presence was confirmed by 2D-HPTLC and in situ derivatization with iodine.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Multidisciplinary
Prawez Alam, Mounir M. Salem-Bekhit, Fakhria A. Al-Joufi, Mohammed H. Alqarni, Faiyaz Shakeel
Summary: The novel antitumor drug cabozantinib was evaluated using validated green RP-HPTLC and NP-HPTLC techniques in marketed tablets and capsules. The RP-HPTLC demonstrated higher sensitivity for quantification of CZN compared to NP-HPTLC, making it a more suitable technique for analyzing pharmaceutical dosage forms containing CZN.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Article
Biochemical Research Methods
Thi Kieu Tien Do, Ilona Trettin, Mona Hanni, Eike Reich
Summary: Predicting chromatographic results is challenging, but High-Performance Thin-Layer Chromatography (HPTLC) offers reproducibility and the ability to generate large datasets for predictive modeling. This study demonstrates the relationship between HPTLC RF and molecular chemical properties, and encourages the use of computational methods for HPTLC data evaluation.
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
(2023)
Article
Engineering, Marine
Yu Wang, Hamn-Ching Chen, Arjen Koop, Guilherme Vaz
Summary: In this work, an extensive verification and validation study is conducted to assess the accuracy and credibility of CFD simulations for the hydrodynamic response of a Floating Offshore Wind Turbine (FOWT) with a semisubmersible platform under regular waves. The study incorporates a dynamic mooring model to accurately simulate the mooring system. Various metrics, including surge, heave, and pitch RAOs, are investigated at different frequencies, and the numerical uncertainties are quantified based on statistical, iterative, and discretization uncertainty sources. The results show that the coupled CFD-FEM code is accurate for simulating FOWT under regular waves, and guidelines for best practices in the verification and validation study of CFD simulations for floating structures under regular waves are provided.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)