Article
Biochemical Research Methods
Nuanyi Liang, Lucie Necasova, Yuan-Yuan Zhao, Jonathan M. Curtis
Summary: This study describes an analytical-semipreparative scale counter-current chromatography (CCC) method for enriching multiple ganglioside homologues, demonstrating successful separation of porcine gangliosides using adjustments in solvent system composition and elution modes.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Joao P. Mendes, Ricardo J. S. Silva, Mikael Berg, Linda Mathiasson, Cristina Peixoto, Paula M. Alves, Manuel J. T. Carrondo
Summary: Virus-based biologicals, particularly oncolytic viruses, have promising applications in the field of medicine. The use of periodic counter-current chromatography (PCC) has shown to be an efficient method for intermediate purification of oncolytic adenovirus, leading to high virus recoveries and impurity reductions. The study also highlights the importance of process stability and automation in ion-exchange chromatography capture.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Mechanics
Ellen K. Luckins, James M. Oliver, Colin P. Please, Benjamin M. Sloman, Robert A. Van Gorder
Summary: The study focuses on endothermic reactions and the flow of granular solid reactants powered by hot gas counter-current flow. It explores various heat-transfer rate values and identifies six distinguished limits, with a diffusive boundary layer for high temperatures. The research shows that the direction of net heat flux affects the solution structure in the single-temperature limit.
JOURNAL OF FLUID MECHANICS
(2022)
Review
Chemistry, Analytical
Yuru Guo, Shengqiang Tong, Keqing Zhang, Jizhong Yan
Summary: Counter-current chromatography, as a liquid-liquid partition chromatography technique, has advantages in large sample loading capacity without irreversible adsorption, and its separation process is greatly affected by factors including partition coefficient, two-phase solvent systems, apparatus, and operating parameters. The development of theoretical research to master the principles of counter-current chromatographic separations is essential to promote applications. Recent progress in separation prediction methods focuses on partition coefficient prediction, modeling the thermodynamic process, and modeling the dynamic process of counter-current chromatography.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Engineering, Chemical
Hoon Choi, Nathan E. Soland, Marisa R. Moss, Jian Liu, Ryan R. Prestangen, Rui Katahira, Suh-Jane Lee, Michael R. Thorson, Charles J. Freeman, Eric M. Karp
Summary: In this study, a predictive model called the Cell Utilized Partitioning (CUP) model was developed to accurately predict the effluents in multicomponent separations in counter-current chromatography (CCC). The model was compared with experimental data and showed good agreement. The simulations using the CUP model also investigated the influence of operating and intrinsic parameters on the yield and productivity, as well as compared the separation performances of different modes under various conditions. The results provide generalized optimization and scaleup guidelines for maximizing CCC performance in research and industrial processes.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Mateusz Dembowski, John E. Rowley, Kevin Boland, Janelle Droessler, David A. Hathcoat, Alexandria Marchi, George S. Goff, Iain May
Summary: This study evaluates the application of High-Speed Counter-Current Chromatography (HSCCC) in the separation of non-radioactive rare earth elements (REEs). The results demonstrate the feasibility and effectiveness of HSCCC in separating a subset of REEs, providing potential applications in radioelement separation chemistry.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biotechnology & Applied Microbiology
Ce Shi, Qi-Lei Zhang, Biao Jiao, Xu-Jun Chen, Ran Chen, Wei Gong, Shan-Jing Yao, Dong-Qiang Lin
Summary: Continuous capture with affinity chromatography is crucial for monoclonal antibody manufacturing. Three different approaches were studied for process optimization, with the model-based approach found to be superior. Optimal residence time for maximum productivity was identified at the boundary of two productivity phases. Comprehensive effects of operating parameters were evaluated, resulting in the prediction of an operation space. Achieving high productivity and capacity utilization was demonstrated using model-assisted tools in continuous capture.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Narges Lali, Peter Satzer, Alois Jungbauer
Summary: The trend in the biopharmaceutical industry is shifting towards continuous manufacturing, requiring traceability of materials. Understanding process dynamics and residence time distribution (RTD) is important for continuous biomanufacturing. In this study, the RTD of three-column periodic counter-current chromatography (PCC) was investigated using a fluorescent-labeled antibody as an inert tracer.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Jinxing Guo, Luqi Li, Zhuo Liu, Yi Zhou, Jinrong Wang, Zhi Yang
Summary: A multi-dimensional chromatography system was developed to isolate flavonoids, phenylpropanoids, and thymol from Thymus quinquecostatus Celak. This system integrated normal-phase flash chromatography and counter-current chromatography. The study used two-dimensional separations with different solvent systems to separate the constituents of Thymus quinquecostatus Celak, and successfully isolated multiple compounds.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Construction & Building Technology
Graeme Flett, Nick Kelly
Summary: This paper presents a new method for generating occupancy and linked demand data using a probabilistic model to better match the behavior of specific households, without requiring additional calibration data.
ENERGY AND BUILDINGS
(2021)
Review
Chemistry, Analytical
Jun-Xian Jing, Dong Pei, Duo-Long Di, Xin-Yi Huang
Summary: In counter-current chromatography, the separation efficiency relies on the partitioning ability and partition coefficient, with the choice of solvent system playing a critical role. The development history and characteristics of three-phase solvent system provide new insights for the study of counter-current chromatography.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Joao P. Mendes, Magnus Bergman, Anita Solbrand, Cristina Peixoto, Manuel J. T. Carrondo, Ricardo J. S. Silva
Summary: Replacing batch unit operations of biopharmaceuticals by continuous manufacturing with periodic counter-current chromatography (PCC) is a mature concept. This study describes the implementation of continuous adeno-associated virus (AAV) affinity capture using a four-column PCC process, which achieved nearly threefold improvement in productivity compared to the optimized batch process.
Article
Engineering, Mechanical
Sergey Pashkov, Sergey A. Zelepugin
Summary: This paper presents a probabilistic approach to finite element modelling of dynamic fracture problems, using the normal distribution law to model internal structural defects and inhomogeneities. The approach can simulate the initiation and development of cracks in the material at different scales.
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART C-JOURNAL OF MECHANICAL ENGINEERING SCIENCE
(2022)
Article
Biochemical Research Methods
Shunjie Zhang, Haijun Chen, Xiaodie Deng, Hailu Chen, Chanjuan Guo, Li Wan, Aihua Peng, Lijuan Chen
Summary: Counter-current chromatography (CCC) is a widely used liquid-liquid separation technique, and high aspect ratio rectangular horizontal (RH) tubing has shown advantages in improving resolution and throughput. This study verifies the high stationary phase retention and throughput of RH tubing on a semi-preparative CCC apparatus, and successfully manufactures a lighter and larger volume semi preparative bobbin with thin-wall RH tubing.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Mathematics, Applied
Filipa M. Valdeira, Ricardo Ferreira, Alessandra Micheletti, Claudia Soares
Summary: This study proposes a shape fitting/registration method based on Gaussian processes, which is suitable for shapes with extensive regions of missing data. The method outperforms existing approaches in dealing with missing data and is compared to state-of-the-art registration methods and current approaches for registration with Gaussian processes.
SIAM JOURNAL ON MATHEMATICS OF DATA SCIENCE
(2023)
Article
Health Care Sciences & Services
Joost de Folter, Mark Trusheim, Pall Jonsson, Sarah Garner
INTERNATIONAL JOURNAL OF TECHNOLOGY ASSESSMENT IN HEALTH CARE
(2018)
Article
Medical Informatics
Joost de Folter, Hulya Gokalp, Joanna Fursse, Urvashi Sharma, Malcolm Clarke
BMC MEDICAL INFORMATICS AND DECISION MAKING
(2014)
Article
Biochemical Research Methods
Joost de Folter, Ian A. Sutherland
JOURNAL OF CHROMATOGRAPHY A
(2009)
Article
Biochemical Research Methods
Ian Sutherland, Peter Hewitson, Joost de Folter
JOURNAL OF CHROMATOGRAPHY A
(2011)
Article
Computer Science, Software Engineering
Joost de Folter, Timothy Cribbin
JOURNAL OF VISUAL LANGUAGES AND COMPUTING
(2012)
Article
Engineering, Biomedical
Matthew E. H. White, Ludwig R. Sinn, D. Marc Jones, Joost de Folter, Simran Kaur Aulakh, Ziyue Wang, Helen R. Flynn, Lynn Krueger, Pinkus Tober-Lau, Vadim Demichev, Florian Kurth, Michael Muelleder, Veronique Blanchard, Christoph B. Messner, Markus Ralser
Summary: A technique named 'OxoScan-MS' allows for the large-scale quantification of glycopeptides in plasma samples. By applying this technique, the study identified differential glycosylation in COVID-19 patients compared to healthy controls in various disease-relevant plasma glycoproteins. This technique has the potential for the quantitative mapping of glycoproteomes in a large number of samples.
NATURE BIOMEDICAL ENGINEERING
(2023)
Proceedings Paper
Engineering, Biomedical
Malcolm Clarke, Joost de Folter, Charles Palmer, Vivek Verma
2013 IEEE POINT-OF-CARE HEALTHCARE TECHNOLOGIES (PHT)
(2013)
Article
Biochemical Research Methods
IA Sutherland, J de Folter, P Wood
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
(2003)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)