Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1218, Issue 14, Pages 1795-1801Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2011.02.005
Keywords
Oligonucleotide; ICP-MS; AuNP-based amplification
Funding
- National Science Council of the Republic of China (Taiwan)
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We have developed a sensitive gold nanoparticle (AuNP)-based inductively coupled plasma mass spectrometry (ICP-MS) amplification and magnetic separation method for the detection of oligonucleotide sequences. The assay relies on (i) the sandwich-type binding of two designed probe sequences that specifically recognize the target oligonucleotide sequences, (ii) magnetic bead separation, and (iii) AuNP-based ICP-MS amplification detection. To enhance the analytical signal and minimize the background signal resulting from nonspecific binding, we performed a series of experiments to evaluate the effects of various parameters (the concentration of the capture probe; the time required for hybridization; the number of washings required to eliminate nonspecific binding) on the oligonucleotide detection. Under the optimized conditions, the detection limit was 80 zmol (corresponding to 1.6fM of the target sequence in a sample volume of 50 mu L). Moreover, it employs a shorter hybridization step and ICP-MS, this procedure is relatively simple and rapid (ca. 1.5 h). Based on the analytical results obtained using complementary and mismatched sequences, our method exhibits good performance in distinguishing complementary and random oligonucleotides. Compared with the gold standard methodology (plaque assay) for the quantification of dengue virus, our method has the capability to allow early detection of dengue virus in complicated and small-volume samples, with high specificity, good analytical sensitivity, and superior time-effectiveness. (C) 2011 Elsevier B.V. All rights reserved.
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