Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Biochemical Research Methods
Guangyong Yang, Jingxi Guo, Hui Yuan, Lei Sun, Lina Sha
Summary: In this study, a method based on ultra-performance convergence chromatography-triple quadrupole mass spectrometry (UPC2-MS/MS) was developed to detect 63 glucocorticoids in healthy foods. The method showed good linearity, low detection limits, and better selectivity and resolution compared to the traditional reverse-phase liquid chromatography-mass spectrometry (RPLC-MS/MS) method. The study provides new technical support for assessing the risk of exposure to glucocorticoids in healthy foods.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Xiangdong Ding, Chao Liu, Wei Yu, Zhongling Liu
Summary: A green, efficient and easy method combining magnetic ionic liquid-based liquid-liquid microextraction (MIL-based LLME) with ultraperformance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UPLC-QqQ/ MS2) was developed for simultaneous determination of neurotransmitters (NTs) in biosamples. The selected magnetic ionic liquid [P6,6,6,14]2[CoCl4] showed advantages of visual recognition, paramagnetic behavior and higher extraction efficiency. The proposed method achieved facile magnetic separation of MIL containing analytes from matrix and successfully extracted and determined 20 NTs in human cerebrospinal fluid and plasma samples. The excellent analytical performance suggests the broad potential of this method for clinical diagnosis and therapy of neurological diseases.
Article
Environmental Sciences
Benjamin H. Maskrey, Karl Dean, Nadine Morrell, Andrew D. Turner
Summary: A simple and rapid UHPLC-MS/MS method has been developed for quantifying pharmaceuticals and metabolites in bivalve molluscs, demonstrating good performance in terms of extraction efficiencies and sensitivity.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2021)
Article
Chemistry, Analytical
Selina Tisler, David Pattison, Jan H. Christensen
Summary: Matrix effects pose challenges for accurate measurements with LC ESI-MS. This study developed a three-step method to evaluate and compensate for matrix effects in enriched wastewater extracts. The method involved determining the optimal relative enrichment factor, correcting matrix effects at higher REFs, and predicting and correcting residual structure-specific matrix effects.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiao-wei Zhang, Yu-ting Chen, Xue Feng, Lu-ya Li, Ke-wei Song, Yu-peng Sun, Guo-hua Zhang, Lan-tong Zhang
Summary: In this study, the metabolism of celastrol in vivo and in vitro was comprehensively characterized using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. A total of 43 metabolites were identified and characterized, and it was found that the biotransformation capacity of the intestinal tract exceeded that of the liver. These findings provide a basis for understanding the pharmacological mechanisms of celastrol and have potential implications for clinical applications.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemical Research Methods
Maarten Degreef, Eleanor M. Berry, Adrian Covaci, Kristof E. K. Maudens, Alexander L. N. van Nuijs
Summary: This study compares two easy-to-use screening methods for routine toxicological analysis. After analyzing 105 medico-legal case samples, it was found that both methods are effective for screening most compounds in toxicological analysis. The semi-quantitative analysis using the tMRM method with a small number of labeled internal standards yielded excellent results, while the semi-quantitative analysis using the QTOF method was more time-consuming.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Analytical
Ahmed Mostafa, Heba Shaaban, Abdulmalik Alqarni, Raneem Al-Ansari, Ahlam Alrashidi, Fatima Al -Sultan, Mohammed Alsulaiman, Fatimah Alsaif, Omer Aga
Summary: Occurrence of pharmaceuticals in wastewater can lead to contamination and health hazards in water environments. To monitor these pharmaceuticals, a eco-friendly, sensitive, robust and selective UHPLC-MS/MS method using SPE for sample preparation was developed. The method achieved satisfactory extraction efficiencies, recoveries, precision and low limits of detection. It successfully analyzed 32 pharmaceuticals in wastewater and assessed their environmental risk. The study emphasized the importance of monitoring these compounds for future mitigation actions.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Applied
Chengfei Wang, Xiaowei Li, Fugen Yu, Yingyu Wang, Dongyang Ye, Xue Hu, Lan Zhou, Jingjing Du, Xi Xia
Summary: The developed method can be used to detect multiple veterinary drug residues in eggs with high accuracy and stability, and has been successfully applied in real samples. The dietary exposure risk assessment based on survey results indicated that the evaluated daily intake and percentage of acceptable daily intake were at toxicologically acceptable levels.
Article
Environmental Sciences
Sofia Barreales-Suarez, Stephane Azoulay, Miguel Angel Bello-Lopez, Rut Fernandez-Torres
Summary: In this study, an uptake assay of pharmaceuticals by Juncus sp. and Salicornia europaea was conducted at three concentration levels. The results showed that plants irrigated at high levels of pharmaceuticals were significantly affected, while plants at other levels displayed no apparent physiological effects. Ibuprofen was not detected in any sample, and some pharmaceuticals were not detected in Salicornia europaea samples.
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Chemistry, Analytical
Rui Guo, Qisheng Zhong, Jiaqi Liu, Peiming Bai, Zongpeng Wang, Jieling Kou, Peijie Chen, Jie Zhang, Bo Zhang
Summary: In this study, a method called 2D-LC/TQMS was developed for simultaneous analysis of hydrophilic and hydrophobic metabolites using the combination of HILIC and RPLC. The method can detect 417 metabolites involved in various metabolic pathways with good reproducibility, linearity, and wide linear range. It was tested using different biological samples and applied to a bladder cancer case/control study, identifying 30 differential metabolites related to carbohydrate and amino acid metabolism.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Applied
Carmen Maria Moscoso-Perez, Veronica Fernandez-Gonzalez, Jorge Moreda-Pineiro, Purificacion Lopez Mahia, Soledad Muniategui-Lorenzo
Summary: Although the European Parliament has set limits for the environmental quality of organotin compounds (OTs) in Directive 2013/39/EU, the marine environment continues to be affected due to global maritime circulation. OTs accumulate in fish and seafood, making them the main source of OTs in human consumption. A robust and fast method for quantifying OTs in fish and seafood, using ultrasound-assisted extraction and gas chromatography-tandem mass spectrometry, has been validated and applied.
FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE
(2023)
Article
Environmental Sciences
Yaotian Wang, Haiyan Zhang, Xingwang Hou, Qing Zhang, Weifang Chen, Jianbo Shi, Guibin Jiang
Summary: A sensitive method for simultaneous determination of nine polyCBD congeners in shellfish was developed and applied to samples from markets in eight coastal cities in China. The results showed low contamination of HCBD in the marketed shellfish and fish, laying the groundwork for future research on the environmental behavior and exposure risks of polyCBDs in aquatic biotas.
ENVIRONMENTAL POLLUTION
(2021)
Article
Multidisciplinary Sciences
Estrella Sanz Rodriguez, Shing Lam, Gregory G. Smith, Paul R. Haddad, Brett Paull
Summary: This study described a method for the direct, robust, accurate, and highly sensitive detection of oxyhalide species in natural waters, including seawater, using suppressed ion chromatography coupled with mass spectrometry. The method demonstrated practical utility in monitoring changes to seawater composition following disinfection treatments, with iodate, bromate, and chlorate detected as the main oxyanionic disinfection by-products.
Editorial Material
Environmental Sciences
Ricardo A. Torres-Palma, Felix Hernandez, Miguel M. Santos, Vitor J. P. Vilar
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Analytical
David Fabregat-Safont, Maria Ibanez, Felix Hernandez, Juan Sancho
Summary: Real-time analysis of exhaled breath has gained significant attention in recent years. A newly developed Atmospheric Breath Analysis Probe (ABAP) has been designed to be compatible with Waters mass spectrometry instruments without the need for expensive modifications. The probe is cost-effective, easy to use, and has been successfully applied for compound analysis in human breath and food.
MICROCHEMICAL JOURNAL
(2022)
Article
Environmental Sciences
F. J. Lopez, E. Pitarch, A. . Botero-Coy, D. Fabregat-Safont, M. Ibanez, J. M. Marin, A. Peruga, N. Ontanon, S. Martinez-Morcillo, A. Olalla, Y. Valcarcel, I. Varo, F. Hernandez
Summary: The study investigated the impact of a wastewater treatment plant from Madrid on the aquatic environment, focusing on pharmaceuticals and pesticides, including their removal efficiency from the water. Results showed that the effluent from the treatment plant had an impact on the water quality of the Manzanares River, with certain pharmaceuticals posing potential risks to aquatic organisms.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Efraim A. Serna-Galvis, Ana M. Botero-Coy, Milton Rosero-Moreano, Judy Lee, Felix Hernandez, Ricardo A. Torres-Palma
Summary: This study characterized hospital wastewater from three cities in Colombia and found the presence of pharmaceutical residues. Although individual environmental risks were low, the mixture of pharmaceuticals in the wastewater posed potential environmental hazards. Therefore, it is necessary to develop efficient processes for the removal of pharmaceuticals from wastewater.
Article
Environmental Sciences
Werner Brack, Damia Barcelo Culleres, Alistair B. A. Boxall, Helene Budzinski, Sara Castiglioni, Adrian Covaci, Valeria Dulio, Beate I. Escher, Peter Fantke, Faith Kandie, Despo Fatta-Kassinos, Felix J. Hernandez, Klara Hilscherova, Juliane Hollender, Henner Hollert, Annika Jahnke, Barbara Kasprzyk-Hordern, Stuart J. Khan, Andreas Kortenkamp, Klaus Kuemmerer, Brice Lalonde, Marja H. Lamoree, Yves Levi, Pablo Antonio Lara Martin, Cassiana C. Montagner, Christian Mougin, Titus Msagati, Joerg Oehlmann, Leo Posthuma, Malcolm Reid, Martin Reinhard, Susan D. Richardson, Pawel Rostkowski, Emma Schymanski, Flurina Schneider, Jaroslav Slobodnik, Yasuyuki Shibata, Shane Allen Snyder, Fernando Fabriz Sodre, Ivana Teodorovic, Kevin V. Thomas, Gisela A. Umbuzeiro, Pham Hung Viet, Karina Gin Yew-Hoong, Xiaowei Zhang, Ettore Zuccato
Summary: A suggestion to establish an international science-policy body has been made to address the severe threats of chemical pollution crisis. Immediate action is crucial and should be informed by reliable scientific knowledge and data. Challenges for such a body include global knowledge production, coverage of all hazardous chemicals, a one-health perspective, and solution-oriented assessments based on systems thinking.
ENVIRONMENTAL SCIENCES EUROPE
(2022)
Article
Biology
David Fabregat-Safont, Maria Mata-Pesquera, Manuela Barneo-Munoz, Ferran Martinez-Garcia, Marie Mardal, Anders B. Davidsen, Juan Sancho, Felix Hernandez, Maria Ibanez
Summary: Synthetic cannabinoids are widely used psychoactive drugs. This study evaluated the metabolism and pharmacokinetics of two synthetic cannabinoids in rats, finding differences in their reactions and distribution in different tissues.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Analytical
David Izquierdo-Sandoval, David Fabregat-Safont, Leticia Lacalle-Bergeron, Juan V. Sancho, Felix Hernandez, Tania Portoles
Summary: The combination of GC with IMS-HRMS is considered a promising approach for monitoring (semi)volatile compounds in complex matrices. Utilizing soft ionization and ion mobility separation, it enables rapid, sensitive, and efficient wide-scope screening while improving (tentative) identifications. With the assessment of 264 organic pollutants, GC-APCI-IMS-HRMS demonstrated robust identification power in complex-matrix samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Andrea Estevez-Danta, Lubertus Bijlsma, Ricardo Capela, Rafael Cela, Alberto Celma, Felix Hernandez, Unax Lertxundi, Joao Matias, Rosa Montes, Gorka Orive, Ailette Prieto, Miguel M. Santos, Rosario Rodil, Jose Benito Quintana
Summary: This study investigated the use of illicit drugs and licit substances of abuse during the COVID-19 pandemic restrictions in several locations in Spain and Portugal using wastewater-based epidemiology. The results showed widespread use of substances such as cannabis and cocaine, with limited use of methamphetamine. The study also confirmed two instances of ecstasy dumping in the sewage network.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Roque Serrano, Juan C. Navarro, Carlos Sales, Tania Portoles, Oscar Monroig, Joaquin Beltran, Felix Hernandez
Summary: Very long-chain polyunsaturated fatty acids (VLC-PUFA) play an important role in the development of the nervous system, retinal function, and reproductive processes in vertebrates. The effect of high vegetable oil aquafeed formulations on VLC-PUFA levels has not been fully understood.
SCIENTIFIC REPORTS
(2022)
Article
Environmental Sciences
Marina Campos-Manas, David Fabregat-Safont, Felix Hernandez, Eva de Rijke, Pim de Voogt, Annemarie van Wezel, Lubertus Bijlsma
Summary: Wastewater-based epidemiology (WBE) is a method that assesses levels of biomarkers in wastewater to gather unique information on drug consumption and human exposure to chemicals. This study developed an advanced analytical method for quantifying pesticide biomarkers in urban wastewater and applied it to samples collected across Europe, showing the presence of specific metabolites.
Article
Chemistry, Analytical
David Fabregat-Safont, Elisa Gracia-Marin, Maria Ibanez, Elena Pitarch, Felix Hernandez
Summary: The research on antibiotics occurrence in the aquatic environment has gained attention due to their potential negative effects, such as bacterial antibiotic-resistance. Antibiotic residues are not completely removed in wastewater treatment plants and end up in water bodies. Analytical methodologies are needed to determine antibiotics in influent and effluent wastewater for various purposes, such as environmental risk assessment and estimating removal efficiencies.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biotechnology & Applied Microbiology
Johanna Zambrano, Pedro Antonio Garcia-Encina, Felix Hernandez, Ana M. Botero-Coy, Juan J. Jimenez, Ruben Irusta-Mata
Summary: The study investigated the mechanisms involved in the removal of four veterinary antibiotics (TTC, CPF, SDZ, SMX) in synthetic wastewater using microalgae-bacteria consortia dominated by Scenedesmus almeriensis. The overall removal efficiency of the antibiotics was determined, and a pseudo-first order irreversible model was applied to analyze the degradation rates. Biosorption was identified as the main mechanism for all four antibiotics, followed by biodegradation in the cases of TTC and SDZ. CPF showed no removal via biodegradation, while SMX did not undergo hydrolysis or photolysis. The study demonstrates the potential use of microalgae as an ecofriendly wastewater treatment process.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Food Science & Technology
Leticia Lacalle-Bergeron, David Izquierdo-Sandoval, Alfredo Fernandez-Quintela, Maria P. Portillo, Juan Vicente Sancho, Felix Hernandez, Tania Portoles
Summary: Untargeted metabolomics with IMS-HRMS was used to study the effects of resveratrol and pterostilbene supplementation on the metabolic fingerprint of the liver in rats with induced liver steatosis. Different alterations in liver metabolism were observed with the two supplements. Resveratrol affected lysophospholipid metabolism, while pterostilbene affected vitamins and derivatives. The use of IMS provided additional structural information for confident identification.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Environmental Sciences
Nikolaos Rousis, Richard Bade, Ivan Romero-Sanchez, Jochen F. Mueller, Nikolaos S. Thomaidis, Kevin Thomas, Emma Gracia-Lor
Summary: The market for illicit drugs and new psychoactive substances (NPS) has increased significantly and festivals are identified as high-risk environments for substance use. Traditional surveillance methods have limitations, and wastewater-based epidemiology (WBE) proves to be a cost-effective and timely alternative. Utilizing WBE, a study was conducted in a large city in Spain during the New Year period and a summer Festival, identifying high consumption rates of specific NPS and illicit drugs, as well as dynamic changes in their use patterns over a six-month period. The study highlights the importance of WBE in complementing public health information and providing insights into drug use trends at festivals.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Engineering, Environmental
Frieberg Kim, Gago-Ferrero Pablo, Bijlsma Lubertus, Ahrens Lutz, Wiberg Karin, Hernandez Felix, Oskarsson Agneta, Lundqvist Johan
Summary: Indirect potable reuse of treated wastewater for drinking water production is a viable solution to water scarcity. Disinfection is effective in reducing microbial hazards, but it can lead to the formation of disinfection by-products. This study assessed the chemical hazards in a system where chlorination was used for disinfection, and found a significant reduction in bioactive pollutants in the finished drinking water. The findings contribute valuable knowledge to increase the reuse of treated wastewater for drinking water production.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)