Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1217, Issue 26, Pages 4255-4266Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2010.04.055
Keywords
Molecularly imprinted micro-solid phase extraction silver fiber; Enantioseperation; D- and L-Thyroxine; Differential pulse anodic stripping voltammetry; Real sample analysis
Funding
- Banaras Hindu University, Varanasi
- Council of Scientific and Industrial Research (CSIR), New Delhi [01 (2319)/09/EMR-II]
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Thyroxine is a known disease biomarker which demands a highly sensitive and selective technique to measure ultratrace level with enantiodifferentiation of its optical isomers (D- and L-), in real samples. In this work, an approach of hyphenation between molecularly imprinted micro-solid phase extraction and a complementary molecularly imprinted polymer-sensor was adopted for enantioseparation, preconcentration, and analysis of D- and L-thyroxine. In both techniques, the same imprinted polymer, coated on a vinyl functionalized self-assembled monolayer modified silver wire, was used as the respective extraction fiber as well as sensor material. This combination enabled enhanced preconcentration of test analyte substantially so as to achieve the stringent limit [limit of detection: 0.0084 ng mL(-1) RSD = 0.81%, S/N = 3 (D-thyroxine); 0.0087 ng mL(-1) RSD = 0.63%, S/N = 3 (L-thyroxine)] of clinical detection of thyroid-related diseases, without any problems of non-specific false-positive contribution and cross-reactivity. (c) 2010 Elsevier B.V. All rights reserved.
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