Article
Chemistry, Analytical
Goncalo J. Gouveia, Amanda O. Shaver, Brianna M. Garcia, Alison M. Morse, Erik C. Andersen, Arthur S. Edison, Lauren M. McIntyre
Summary: The study developed stable and sustainable reference materials (RM) using the iterative batch averaging method (IBAT), which was then applied in a metabolomics experiment to estimate the proportion of sample variation.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Weizhu Chen, Wenhui Jin, Hua Fang, Hui Chen, Xiaoying Chen, Yiping Zhang, Zhuan Hong
Summary: This paper describes the development of a taurine purity certified reference material (CRM) based on ISO principles. The CRM was characterized using mass balance and quantitative nuclear magnetic resonance spectroscopy. The results showed that the CRM had sufficient homogeneity and remained stable under certain conditions. It can validate analytical measurements in laboratories to ensure accuracy, traceability, reliability, and comparability of results.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Jian Zhou, FuKai Li, Min Wang, ChenBin Yan, MengRui Yang, TongTong Wang, LiYuan Zhang
Summary: A novel purity certified reference material (CRM) of clorprenaline (GBW 09255) has been developed using the mass balance method, differential scanning calorimetry (DSC), and quantitative nuclear magnetic resonance spectroscopy (QNMR). The CRM has been characterized and the uncertainty evaluated, showing good homogeneity and stability. This developed CRM can effectively support method validation, proficiency testing, and nationwide quality monitoring.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Chen He, Qin Gao, Changwen Ye, Guotao Yang, Pengfei Zhang, Rongchao Yang, Qing Zhang, Kang Ma
Summary: In this paper, an improved method was established for the determination of three impurities in vinyl acetate, using the mass balance method. Gas chromatography tandem high-resolution mass spectrometry was used to identify the impurities as methyl acetate, ethyl acetate, and vinyl propionate. The purity of vinyl acetate was determined to be 99.90% with an expanded uncertainty of 0.30%, and the total content of organic impurities was 0.08% with a relative correction factor of 1.23%. The vinyl acetate reference material has been certified as GBW (E) 062710 in China.
Article
Biochemical Research Methods
Athira Joseph, Gangasani Jagadeesh Kumar, Sachin Dattram Pawar, Bhakti Umesh Hirlekar, Prasad Bharatam, Satyanand Konda, Mohana Krishna Reddy Mudiam, Upadhyayula Suryanarayana Murty, P. L. Sahu, Sachin Dubey, P. Radhakrishnanand, Daya Raju Adye, Roshan M. Borkar, Choppari Thirupathi, Pramod Kumar
Summary: This study focused on the development and validation of a chiral HPLC method for p-hydroxy prenylamine reference material, allowing for more extensive characterization compared to existing methods. The synthesized batches of p-hydroxy prenylamine showed high purity and could serve as valuable reference material for doping testing.
DRUG TESTING AND ANALYSIS
(2022)
Article
Chemistry, Analytical
Mengrui Yang, Shan Li, Min Wang, Jian Zhou, Quanhui Liu, Liyuan Zhang, Tongtong Wang
Summary: A new egg powder certified reference material was developed for the measurement of florfenicol residue, with a certified value assigned and stability testing conducted. The CRM showed homogeneity within and between bottles, and its uncertainty was assessed based on characterization, homogeneity, and stability contributions. This CRM has great potential for method validation and quality control in florfenicol determination in eggs.
MICROCHEMICAL JOURNAL
(2021)
Article
Food Science & Technology
Andrew D. Turner, Daniel G. Beach, Amanda Foss, Ingunn A. Samdal, Kjersti L. E. Lovberg, Julia Waack, Christine Edwards, Linda A. Lawton, Karl J. Dean, Benjamin H. Maskrey, Adam M. Lewis
Summary: A reference material for cyanotoxins in shellfish has been successfully prepared, containing microcystins and nodularins. The total soluble toxin concentration was determined to be 2425 +/- 575 μg/kg, with an average of 43 +/- 9% of bound toxins present. This reference material provides a valuable resource for monitoring, managing, and mitigating the risk of cyanotoxins.
Article
Biochemical Research Methods
Marco Ballotari, Francesco Tau, Giulia Tolle, Elisa Danese, Romolo M. Dorizzi, Franco Tagliaro, Rossella Gottardo
Summary: A new UPLC-MS/MS method was developed for the determination of digoxin and digitoxin in plasma. The method showed good sensitivity and selectivity, with excellent linearity, accuracy, precision, and recovery. There was a negative bias when compared to the immunoassay.
Article
Food Science & Technology
Li Li, Peng Li, Yu Wu, Jin Ye, Zongwang Li, Songxue Wang
Summary: Matrix certified reference materials are crucial for analytical method validation and reliable measurement results. A certified reference material for whole-wheat flour has been developed to accurately measure the main Fusarium mycotoxins. This CRM is homogeneous, stable, and suitable for quality control and method validation of mycotoxin quantification in whole-wheat flour.
Article
Biochemical Research Methods
Jingfu Zhao, Wenjing Peng, Xue Dong, Yehia Mechref
Summary: Protein-based therapeutics like mAbs play a crucial role in modern medicine, with glycosylation being a key quality attribute affecting the safety and efficacy of biotherapeutics. An LC-MS/MS-based glycoproteomics approach was used to characterize Fc glycans of two NISTmAb samples, revealing differences in galactosylation levels between the modified and unmodified NISTmAb. Additionally, high abundance glycan structures were identified along with relatively low levels of certain glycans and unglycosylated peptides.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Garnet McRae, Donald M. Leek, Juris Meija, Bryn Shurmer, Steven J. Lehotay, Joachim Polzer, Jeremy E. Melanson, Zoltan Mester
Summary: A freeze-dried bovine muscle-certified reference material (CRM), called BOTS-1, containing residues of commonly used veterinary drugs, was produced and certified. Liquid chromatography tandem mass spectrometry (LC-MS/MS) methods were used for value assignment, along with isotope dilution and standard addition approaches. Data from multiple organizations were used for value assignment. Results were obtained through an international inter-laboratory comparison. Primary standards were characterized using quantitative NMR (1H-qNMR). The certified mass fractions of the veterinary drug residues were reported with expanded uncertainties.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Mengrui Yang, Shan Li, Min Wang, Haochuan Shi, Jian Zhou, Liyuan Zhang, Tongtong Wang
Summary: A new matrix CRM for pefloxacin in fish meat powder was developed, prepared from incurred samples and evaluated for homogeneity and stability. Isotope dilution liquid chromatography tandem mass spectrometry was applied for value assignment, with uncertainty evaluated.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Applied
Harischandra R. Naik, M. S. Pallavi, M. Bheemanna, K. PavanKumar, V. Chandra Sekhara Reddy, Udaykumar R. Nidoni, M. Paramasivam, Satish Yadav
Summary: A method for simultaneous determination of 79 pesticide residues in pigeonpea has been developed and validated, with low LOD and LOQ, good recoveries, and applicability for routine monitoring purposes.
Article
Agriculture, Multidisciplinary
Andrew J. Caffrey, Larry A. Lerno, Jerry Zweigenbaum, Susan E. Ebeler
Summary: The isomeric nature of monoterpenyl glycosides makes it difficult to identify intact glycosides, posing a challenge in studying wine fermentation and storage. This study identified linalool, nerol, and geraniol monoterpenyl glycosides in Riesling grapes and monitored their changes during alcoholic fermentations across two years. Glycoside hydrolysis during fermentation could be related to relative concentrations of the corresponding free aglycones.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemical Research Methods
Zhi-mei Yang, Ya-bin Qin, De-yun Zhao, Lan-tao Wang, Ying-ping Tian, Jian-fang Liu
Summary: This study developed a simple and sensitive LC-MS/MS method for therapeutic drug monitoring of digoxin in children with a lower limit of quantitation of 0.2 ng/mL based on 30 μL of plasma. The plasma sample was pretreated using one-step protein precipitation, followed by chromatographic separation on a short C-18 column with a total run time of 2.4 min. Detection was achieved through multiple reaction monitoring using positive ionization mode on a triple quadrupole mass spectrometer. The method was fully validated and applied to determine plasma digoxin concentrations in 43 pediatric patients, proving its appropriateness for routine clinical laboratory practice.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)