Article
Chemistry, Analytical
Xiao-Dong Cheng, Zheng Zhang, Yun-Ping Li
Summary: Green chromatography techniques using low-toxic mobile phase and developing stationary phases with adequate retention and separation under high content water mobile phase have gained increasing attention. In this study, an undecylenic acid-bonded silica stationary phase (UAS) was successfully prepared using thiol-ene click chemistry. The synthesized UAS demonstrated excellent separation ability towards highly polar compounds under the mobile phase of high content water, surpassing commercial C18 and silica stationary phases. Overall, this UAS stationary phase meets the requirements of green chromatography and shows promise for enhanced separation.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Isabelle Kohler, Michel Verhoeven, Rob Haselberg, Andrea F. G. Gargano
Summary: Proteomics and metabolomics have become increasingly important in clinical applications, with RPLC-MS and HILIC-MS techniques playing crucial roles in biomarker discovery and disease diagnosis. While RPLC-MS and HILIC-MS complement each other in resolving the complexity of proteome and metabolites, they also have technical limitations and potential solutions that need to be addressed.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemical Research Methods
Xiaoqing Wang, Jian Cui, Jin Zhou, Shuo Wang, Yiming Gu, Xiaowei Liu, Shudong Wang
Summary: Vinyl modified silica was synthesized through a silane coupling reaction and a poly-acrylamide modified silica (PAM-SIL) stationary phase was prepared via free radical polymerization. The separation performance of the stationary phases could be regulated by controlling the polymer structure, showing potential for future separation and analysis applications.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Fei Wang, Fan Yang, Jiawei Liu, Quan Bai
Summary: This study investigates the retention behavior and mechanism of solutes in hydrophilic interaction chromatography (HILIC) using stoichiometric displacement theory (SDT). A HILIC/reversed-phase liquid chromatography (RPLC) dual-retention mechanism is studied in detail using a β-CD HILIC column. The study finds that a four-parameter equation derived from SDT-R accurately describes the U-shaped curves of solutes with RPLC/HILIC dual-retention mechanisms on the β-CD column, providing a theoretical guide for the development of HILIC and the enhancement of separation efficiency.
Article
Biochemical Research Methods
Xiaoyu Zhou, Hongyan Zhang, Li Wang, Ren'an Wu
Summary: This study developed a dual-functionalized metal-organic framework UIO-PBA&FDP for the efficient enrichment of glycopeptides in proteomics using the HILIC strategy. The UIO-PBA&FDP exhibited excellent hydrophilicity and successfully enriched 359 N-linked glycopeptides from human serum, achieving a high coverage of 104 glycoproteins.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Kaixing Luo, Yu Luo, Yanjuan Liu, Yuefei Zhang, Wei Chen, Zhengwu Bai, Sheng Tang
Summary: In this work, a new strategy was adopted to simultaneously graft two different functional ligands onto the silica surface, resulting in a carbonyldiimidazolium/dodecyl dual-functionalized silica stationary phase (Sil-CDI-DD) using recycled commercial C18 columns as raw materials. The obtained Sil-CDI-DD exhibited enhanced separation selectivity and efficiency, providing multiple interaction sites for diverse target analytes and enabling the favorable resolution of hydrophilic nucleosides/nucleobases and weak polar environmental endocrine disruptors.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Chemistry, Analytical
Qianying Sheng, Meiyan Liu, Minbo Lan, Guangyan Qing
Summary: Hydrophilic interaction liquid chromatography (HILIC) is an attractive complementary method to reversed phase LC for the separation of hydrophilic or strongly polar substances. The advancements in HILIC have significantly improved sensitivity, analyte coverage, throughput, analysis speed, and resolution in bio-separation and bio-analytical chemistry.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Polymer Science
Peter H. Santschi, Wei-Chun Chin, Antonietta Quigg, Chen Xu, Manoj Kamalanathan, Peng Lin, Ruei-Feng Shiu
Summary: Microgels play critical roles in various ocean processes, with exopolymeric substances from marine microbes being a major source. Many types of pollutants, especially hydrophobic ones, have been found to preferentially associate with marine microgels, impacting their transport and fate in the ocean. This review focuses on the interactions between gel-forming EPS and pollutants in the ocean.
Article
Biochemical Research Methods
Haibin Li, Chusheng Liu, Li Zhao, Dongsheng Xu, Tingting Zhang, Qiqin Wang, Deirdre Cabooter, Zhengjin Jiang
Summary: An unmatched or improperly matched sample solvent can distort the peak shape of analytes in chromatography. For retained compounds, pure ACN is the best sample solvent, while for compounds eluting close to the solvent peak, distortions occur with pure ACN. Water in the sample solvent favors electrostatic attraction for charged compounds, leading to increased retention time.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Physical
Justin Engstler, Nicolas Giovambattista
Summary: Water-mediated interactions play important roles in aqueous solutions, affecting processes such as protein folding and nanoparticle aggregation. This study investigates the impact of temperature and pressure on different types of interactions between graphene-based surfaces. The results show that hydrophobic, hydrophilic, and hybrid interactions are all sensitive to pressure, while only hydrophobic interactions are significantly affected by temperature variations. The findings have implications for understanding protein denaturation and biomolecular interactions, as well as for the design of water models for molecular and nanoparticle systems under different thermodynamic conditions.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Food Science & Technology
Zhixing Gu, Xi Nie, Ping Guo, Yuehua Lu, Bo Chen
Summary: An analytical method using HPLC with diode array and evaporative light scattering detectors was established for the simultaneous determination of hydrophobic and hydrophilic compounds in the dried rhizome of Atractylodes macrocephala Koidz. The method provides a new choice for the quality control of traditional Chinese medicines and health foods.
Article
Chemistry, Multidisciplinary
Bing Tang, Wenle Xia, Wei Cai, Jinbin Liu
Summary: In this study, luminescent AuNPs with redshifted emission and good biocompatibility were successfully constructed using an amphiphilic block copolymer template. The strategy involved the formation of uniform clusters of NIR-II AuNPs inside the hydrophobic cores of ABC micelles and the generation of rigid NIR-II AuNPs as ABC unimers on the surface, enabling enhanced biological interactions.
Article
Biochemical Research Methods
N. Yu Chikurova, A. O. Shemiakina, O. A. Shpigun, A. Chernobrovkina
Summary: A series of novel HILIC stationary phases containing both amides and hydroxyl groups were obtained on an aminated silica surface through multicomponent Ugi reaction. These stationary phases showed good separation efficiency and selectivity in chromatographic parameters and model mixtures.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biophysics
Mohammad Saiful Islam, Faradae Renner, Kimberly Foster, Martins S. Oderinde, Kevin Stefanski, Somenath Mitra
Summary: In this study, the direct incorporation of nanographene oxide (nGO) into a hydrophobic drug was found to improve the drug's dissolution performance. The addition of nGO reduced the dissolution time and increased the dissolution rate of the drug. The hydrophilic dispersion of nGO created hydrophilic channels on the drug crystal surfaces, leading to decreased lipophilicity and increased aqueous solubility of the drug.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Multidisciplinary Sciences
Jacob Monroe, Sally Jiao, R. Justin Davis, Dennis Robinson Brown, Lynn E. Katz, M. Scott Shell
Summary: The performance of membranes in water purification, especially in terms of fouling resistance, is influenced by the interactions between solvated species and membrane surfaces. Efforts to create fouling-resistant membranes often focus on hydrophilization of surfaces. However, molecular simulations show that solutes are attracted to both hydrophobic and hydrophilic surfaces, contradicting macroscopic measures which suggest solute affinity is higher on hydrophobic surfaces. This suggests that traditional macroscopic hydrophobicity metrics may not accurately predict solute-surface affinity, and that molecular-scale surface chemical patterning plays a significant role in influencing affinity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Fabrice Gritti
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Chemistry, Analytical
Fabrice Gritti
Summary: The demand for accurate retention time prediction in liquid chromatography has led to the adoption of experimental protocols and software by analysts. Classical approaches for retention time prediction, both inverse and direct methods, are discussed, with a focus on the need for increased accuracy in the future. Statistical methods and quality by design methods based on solid-liquid adsorption and gradient chromatography principles are identified as key elements to improve accuracy in retention time prediction.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Fabrice Gritti, Dzmitry Hlushkou, Ulrich Tallarek
Summary: This study enhances the model of transverse diffusion in multiple-open-tubular columns and finds that the RSD of channel diameters has a limited impact on the optimum plate number of MOTTD columns, but can increase plate height at high velocities. For the fastest analyses at a fixed pressure drop, MOTTD columns with a small structural parameter rho are recommended, while the largest values of rho are required for maximizing performance in MOTTD columns for longer analysis times.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Fabrice Gritti
Summary: Twin column recycling semi-preparative liquid chromatography (TCRLC) has been successfully applied to prepare small amount of pure targeted compounds using gradient elution technique, with automation of the process achieving improved accuracy and efficiency.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Suhas Nawada, Fabrice Gritti
Summary: Mixing multiple solvent streams is crucial for liquid chromatographic separations. Experimental and theoretical analysis show that adjusting mixer volume and design can effectively reduce noise and gradient deformation.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Szabolcs Horvath, Fabrice Gritti, Krisztian Horvath
Summary: The study found that non-uniform columns with particle size gradients can achieve extra bandwidth compression in gradient elution mode, but do not offer any advantage in isocratic elution mode. Efficiency in terms of bandwidth compression can be further increased by optimizing the limiting particle sizes.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Fabrice Gritti, Suhas Nawada
Summary: The current performance of commercially packed liquid chromatography columns is limited by the random structure of the packed bed and by the wall-to-center heterogeneity of its structure. To bridge this gap, a wide inner diameter column with an ordered macroporous structure is printed in three dimensions. Despite the inevitable printing imperfections, minimum reduced plate heights around unity are measured for nonretained analytes.
JOURNAL OF SEPARATION SCIENCE
(2022)
Review
Chemistry, Analytical
M. Farooq Wahab, Fabrice Gritti, Thomas C. O'Haver
Summary: This article presents detailed information on problem solving approaches using discrete Fourier transform (DFT) in the context of separation science and spectroscopic data analysis. The methods discussed are effective in denoising, increasing theoretical plates, reducing asymmetry, and resolving isotopically labeled compounds.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Fabrice Gritti
Summary: This study models the transient diffusion of large biomolecules in ultra-high pressure liquid chromatography using a single fully porous particle. The research reveals that different sized analytes have varying dynamic capacities and equilibrium issues in the chromatography column.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Fabrice Gritti, Nicole Trebel, Alexandra Hoeltzel, Ulrich Tallarek
Summary: This study proposes an alternative method based on molecular dynamics simulations to predict surface excess adsorption and retention factors in liquid chromatography. The calculated retention factors based on the simulated adsorption behavior show good agreement with experimental results. However, there is a systematic error that may be caused by the differences between the simulated system and the actual system, suggesting the need for further investigation.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Fabrice Gritti, Bonnie A. Alden, Justin McLaughlin, Thomas H. Walter
Summary: This study investigated the relationship between retention and mass transfer resistance in hydrophilic interaction liquid chromatography (HILIC) columns packed with the same ethylene-bridged hybrid particles (BEH). The retention volumes and the adsorbed water layer volumes were measured for different HILIC columns. The results showed a correlation between retention volumes and water layer volumes, as well as between water content and mass transfer resistance. The study also found that the peak capacity was higher for the BEH Z-HILIC column compared to the BEH Amide column in gradient elution mode.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Fabrice Gritti, Dzmitry Hlushkou, Ulrich Tallarek
Summary: Multiple-open-tubular columns enabling transverse diffusion (MOTTD) with a mesoporous stationary phase are demonstrated to have advantages over classical particulate columns in terms of band broadening. A MOTTD column with small channel diameters is fabricated by 3D-printing, and its performance is compared to a column with perfect channels. The results show that the 3D-printed MOTTD column outperforms the column with perfect channels in solute dispersion.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Fabrice Gritti
Summary: The demand for characterizing large biomolecules in chromatography raises questions about their absorption and escape kinetics from porous particles. The concentration profiles of different biomolecules across a sub-3 µm BEH Particle in SEC columns were derived, confirming that small molecules and monoclonal antibodies reach thermodynamic equilibrium quickly, while larger biomolecules like dsDNA or VLPs have slower kinetics and lead to peak tailing. These findings have implications for chromatography theories and suggest non-porous particles or monolithic structures for separating large biomolecules in life science.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Fabrice Gritti
Summary: The flow reversal (FR) technique involves reversing the flow direction in a chromatographic column and is used to reveal the origin of resolution limitations in specific columns. By demonstrating long-range flow velocity biases, FR confirms the existence of center-to-wall flow heterogeneities that cause undesired tailing of peaks. The adoption of alternate pumping recycling liquid chromatography (APRLC) allows for the successful separation and quantification of critical impurities in monoclonal antibodies.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Fabrics Gritti, Thomas H. Walter
Summary: This study investigated the phenomenon of retention loss observed for reversed-phase liquid chromatography columns when stopping and resuming the flow, attributing it to pore dewetting in the hydrophobic pore network. Experimental results demonstrate that adjusting various variables can minimize or eliminate this retention loss phenomenon.
LC GC NORTH AMERICA
(2021)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)