Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 46, Pages 8217-8223Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2009.06.058
Keywords
Multi-method; Antibiotics; Milk; Different substance groups; Validation; LC-MS/MS; SPE clean-up
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The presented method is able to analyse 47 substances of the antibiotic groups tetracyclines, quinolones, macrolides, sulfonamides. diamino-pyrimidine derivatives and lincosamides simultaneously in a single analytical run. Applying an in-house validation concept, the validation of the multi-method was successfully accomplished with a low number of experiments. Each substance was validated at least at the concentrations 0.5, 1.0 and 1.5 MRL (maximum residue limit), or respectively, at concentrations as low as possible for substances without MRL. The calculated relevant validation parameters. e.g. the decision limit CC alpha, the detection capability CC beta, the repeatability, the within-laboratory reproducibility and the recovery, are in an acceptable range and in compliance with the requirements of Commission Decision 2002/657/EC. A proficiency test and the implementation of the method by other laboratories were performed successfully. (C) 2009 Elsevier B.V. All rights reserved
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