Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 11, Pages 2071-2078Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2008.05.082
Keywords
Belamcanda chinensis (L.) DC.; Chinese medicine; Fingerprint; HPLC-DAD-MS; Phenols; Quality evaluation
Funding
- Hong Kong SAR, and Nanjing University of Traditional Chinese Medicine [HKUST6419/06M, FJK 2006011]
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A high-performance liquid chromatography coupled with diode array detector and mass spectrometry (HPLC-DAD-MS) method was developed to evaluate the quality of Rhizoma Belamcandae (Belamcanda chinensis (L.) DC.) through establishing chromatographic fingerprint and simultaneous determination of seven phenolic compounds. The analysis was achieved on an Altima C-18 analytical column (250 mm x 4.6 mm i.d. 5 mu m) using linear gradient elution of acetonitrile-0.1% trifluoroacetic acid. The correlation coefficients of similarity were determined from the HPLC fingerprints, and they shared a close similarity. By using an online APCI-MS/MS, twenty phenols were identified. In addition, seven of these phenols including mangiferin, 7-O-methylmangiferin, tectoridin, resveratrol, tectorigenin, irigenin and irisflorentin were quantified by the validated HPLC-DAD method. These phenols are considered to be major Constituents in Rhizoma Belamcandae, and are generally regarded as the index for quality assessment of this herb. This developed method by having a combination of chromatographic fingerprint and quantification analysis could be applied to the quality control of Rhizoma Belamcandae. (c) 2008 Elsevier B.V. All rights reserved.
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