Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1216, Issue 15, Pages 3402-3408Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2009.02.010
Keywords
HPLC/ESI-MS/MS; Phenolic derivatives; Grape skins; Grape seeds; Screening; Flavonoid; Nonflavonoid; Fast-LC
Funding
- Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR)
- Progetto Vinex
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A new method for the identification and the quantification of nonanthocyanin phenolic compounds from six Vitis Vinifera grape varieties native to Sardinia (three native: Vermentino, Malvasia and Cannonau and three non-native types: Chardonnay, Sauvignon and Cabernet Sauvignon; Argiolas vineyard) was developed. This rapid and selective method employs LC/ESI-MS in negative mode. Different solvents extraction and different sorbents for purification were compared to the direct analysis of the initial extracts without further sample preparation. A total of 54 phenolic compounds were identified either in the freeze-dried skins or seeds, including nonflavonoids (hydroxybenzoic and hydroxycinnamic acids and their derivatives, stilbenes) and flavonoids (flavanols, flavonols, dihydroxyflavonols). (C) 2009 Elsevier B.V. All rights reserved.
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