Article
Biochemistry & Molecular Biology
Dorothea Haas, Jana Hauke, Kathrin Schwarz, Lucia Consalvi, Friedrich K. Trefz, Nenad Blau, Georg F. Hoffmann, Peter Burgard, Sven F. Garbade, Juergen G. Okun
Summary: Monitoring phenylalanine concentrations is crucial for managing phenylketonuria. Variations in Phe concentrations between different blood sample types have been observed, with significantly lower Phe levels in erythrocytes compared to plasma. Reliable conversion of dried blood spots into plasma concentrations is essential for therapy recommendations.
Article
Chemistry, Analytical
Pouria Jafari, Sepideh Mohammad Beigi, Farzad Yousefi, Sajjad Aghabalazadeh, Maryam Mousavizadegan, Morteza Hosseini, Saman Hosseinkhani, Mohammad Reza Ganjali
Summary: A colorimetric paper-based biosensor was developed for the sensitive and selective determination of phenylalanine, with the color change indicating the concentration of phenylalanine. The method is easy to operate, provides quick results, has a wide linear range, and can be used for screening PKU in newborns.
MICROCHEMICAL JOURNAL
(2021)
Article
Medicine, General & Internal
Fernando Andrade, Ainara Cano, Maria Unceta Suarez, Arantza Arza, Ana Vinuesa, Leticia Ceberio, Nuria Lopez-Osle, Gorka de Frutos, Raquel Lopez-Oceja, Elena Aznal, Domingo Gonzalez-Lamuno, Javier de las Heras
Summary: The study found that PKU patients have higher urine PAG levels than healthy controls, and there is a significant correlation between urine PAG levels and circulating Phe levels in patients with HPA, suggesting the potential role of urine PAG as a non-invasive biomarker in PKU diagnosis.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Nanoscience & Nanotechnology
Ludovica Maugeri, Maria Anna Messina, Martino Ruggieri, Salvatore Petralia
Summary: An innovative method using photothermal contrast was demonstrated to enhance the analytical sensitivity of enzymatic phenylalanine detection at a solid surface. Gold nanoparticles produced in situ by enzymatic reaction exhibited excellent photothermal effect under green-light stimuli. Optical investigation was conducted to study the enzymatic reaction and nanoparticle formation process. The proposed photothermal-contrast approach showed a sensing range of 10-1000 μM and a detection limit of 3.6 μM for phenylalanine detection.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Lenka Rysava, Milos Dvorak, Pavel Kuban
Summary: This study introduces a simple and convenient concept of automated blood sampling and analysis, allowing for accurate collection of blood samples and automated processing and analysis in a laboratory setting. Using warfarin blood concentration determination as an example, the concept demonstrates excellent analytical performance and wide applicability to various types of blood compounds.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Medical Laboratory Technology
Sarah L. Hogg, Rachel S. Carling, Nathan W. P. Cantley, Gillian Hamilton, Philippa Goddard, Helen Aitkenhead, Robert Barski, Catherine Collingwood, Stuart J. Moat, Helena J. Kemp
Summary: Sapropterin has been approved as a treatment option in the UK for Phenylketonuria patients. The quality of patient-collected dried blood spot (DBS) specimens varies significantly. A study evaluated the impact of introducing evidence-based guidelines for specimen acceptance criteria and found an increase in specimen rejection rates.
ANNALS OF CLINICAL BIOCHEMISTRY
(2023)
Article
Endocrinology & Metabolism
Michael Y. Schakelaar, Eef G. W. M. Lentjes, Timothy S. Q. Visser, Arjan H. Schoneveld, Imo E. Hoefer, Wouter Tiel M. Groenestege
Summary: This study demonstrates that TSH can be accurately measured from dried blood spots (DBS) for the diagnosis of hypothyroidism and hypothryotropinemia. DBSs are stable and cost-effective, making them suitable for telediagnostics, screening programs, patient follow-up, and medication management.
Review
Chemistry, Analytical
Agnieszka Niemiec
Summary: DBS as a new method of sample collection is simple, rapid, and does not require trained personnel to collect samples, playing an important role in newborn screening and toxicology, with a broad development potential in the future.
Article
Medical Laboratory Technology
Lan Gao, Neil Smith, Diksha Kaushik, Sarah Milner, Ronald Kong
Summary: A new VAMS HPLC-MS/MS method was developed for measuring blood phenylalanine (Phe) in PKU patients. Compared to traditional venous blood collection methods, VAMS method is more convenient and provides accurate and reliable results.
CLINICAL BIOCHEMISTRY
(2023)
Article
Medicine, General & Internal
Jessica Beyerl, Raquel Rubio-Acero, Noemi Castelletti, Ivana Paunovic, Inge Kroidl, Zohaib N. Khan, Abhishek Bakuli, Andreas Tautz, Judith Oft, Michael Hoelscher, Andreas Wieser
Summary: Since 2020, SARS-CoV-2 has spread pandemically, infecting over 119 million people and causing over 2.6 million deaths. Symptoms vary greatly and different populations react differently to the disease. Measurement of specific anti-SARS-CoV-2 antibodies is crucial for assessing infection spread and vaccination success.
Article
Medical Laboratory Technology
Rachel S. Carling, Erin C. Emmett, Stuart J. Moat
Summary: This study evaluated the accuracy, imprecision, blood volume, and hematocrit effects of using volumetric devices and conventional DBS cards for measuring phenylalanine and tyrosine. The results showed that volumetric devices performed better in terms of accuracy and were less affected by hematocrit compared to the conventional method.
CLINICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Justyna Spiewak, Ivan Doykov, Apostolos Papandreou, Jenny Haellqvist, Philippa Mills, Peter T. Clayton, Paul Gissen, Kevin Mills, Wendy E. Heywood
Summary: Dried blood spot (DBS) biomarkers are convenient for monitoring specific lysosomal storage diseases (LSDs), but may also have relevance for other LSDs. A study was conducted to determine the specificity and utility of glycosphingolipidoses biomarkers in a DBS cohort of patients with various LSDs. The findings indicated that DBS glucosylceramide isoform profiling increased the specificity for the detection of Niemann-Pick disease type C (NPC), thereby improving diagnostic accuracy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Abhishek Jain, Montana Morris, Elizabeth Z. Lin, Sajid A. Khan, Xiaomei Ma, Nicole C. Deziel, Krystal J. Godri Pollitt, Caroline H. Johnson
Summary: Dried blood spot (DBS) metabolomics has many applications but faces challenges in accurate metabolite quantification due to the hematocrit effect and unknown blood-spotting volumes. This study compared five normalization methods and found that Hb normalization outperformed others in terms of intragroup variation, differential metabolic analysis, and classification accuracy. The study also demonstrated a correlation between specific gravity (SG) and Hb in adults and proposed an equation to use SG as a surrogate for Hb normalization. This comparative study provides a robust methodological platform for future environmental epidemiological studies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Rima Jeske, Uta Merle, Barbara Mueller, Tim Waterboer, Julia Butt
Summary: This study tested the compatibility of dried blood spot (DBS) samples with a bead-based multiplex serology assay for detecting SARS-CoV-2 antibodies. The results showed a high correlation between DBS samples and serum samples, and the classification of SARS-CoV-2 seropositivity remained consistent when adjusting the cutoffs. This technology enables powerful sero-epidemiological studies in resource-poor settings.
Article
Medicine, General & Internal
Verena Damiani, Erika Pizzinato, Ilaria Cicalini, Gianmaria Demattia, Mirco Zucchelli, Luca Natale, Claudia Palmarini, Claudia Di Marzio, Luca Federici, Vincenzo De Laurenzi, Damiana Pieragostino
Summary: The aim of this study was to improve the detection of SARS-CoV-2 nucleocapsid antibodies by developing a simple and reliable DELFIA immunoassay on dried blood spots (DBSs). The results showed that the DBS-DELFIA had a wider dynamic range and higher sensitivity for detecting SARS-CoV-2 nucleocapsid antibodies. Furthermore, a strong correlation was found between the DBS-DELFIA and ELISA immunoassays. Therefore, the combination of dried blood sampling with DELFIA technology provides an easier, minimally invasive, and accurate measurement of SARS-CoV-2 nucleocapsid antibodies in previously infected individuals.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)