Article
Chemistry, Analytical
Feng Yang, Delia Li, Regina Kufer, Lance Cadang, Jennifer Zhang, Lu Dai, Jia Guo, Stefanie Wohlrab, Midori Greenwood-Goodwin, Amy Shen, Dana Duan, Hong Li, Inn H. Yuk
Summary: By employing new strategies and methods, the research team successfully increased the detection sensitivity of residual host cell proteins in drug products and identified the largest number of mouse proteins in a single analysis, filling a significant gap in the field of HCP analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Aiping Wang, Jinran Du, Hua Feng, Jingming Zhou, Yumei Chen, Yankai Liu, Min Jiang, Rui Jia, Yuanyuan Tian, Gaiping Zhang
Summary: This study successfully cloned the BTV1 VP2 gene and expressed the protein in insect cells, leading to the production of effective vaccines. Three anti-BTV1 VP2 monoclonal antibodies were generated, and a novel B-cell epitope on the VP2 protein was identified, laying a foundation for the development of diagnostic and prophylactic methods for bluetongue disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Yair Kaufman, K. C. Hunt, Gabriel Hale, Matthew McClure, David Latulippe, Madhavi Sivan, Jack Wilson, Rachel Dorin, Yury Agroskin, Marty Siwak, Daniele Gerion
Summary: Membrane fouling by monoclonal antibodies (mAbs) is a challenge in virus-filtration processes, and the differences in fouling between different mAbs have not been fully explained. This study found that low concentration aggregates of mAbs can foul membranes through an adsorptive mechanism driven by hydrophobic forces. The charge of the mAbs plays a secondary role in fouling. These insights can potentially be used to mitigate fouling in other virus-filtration and/or ultrafiltration membranes.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Ismael Obaidi, Leticia Martins Mota, Andrew Quigley, Michael Butler
Summary: In an experiment using CHO cell lines to produce mAbs with various protein hydrolysates, it was found that the presence of hydrolysates led to increased cell growth density and mAb productivity. Different hydrolysates had varying effects on the enhancement of mAb productivity, with UPcotton hydrolysate showing the most significant improvement.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Jing-ying Sun, Chun-yan Guo, Guo-rong Wang, Li-ting Yan, Qing Feng, Yan Li, Xue-ping Huo, Xin Xie, Jun Hu, Li-jun Sun
Summary: Our previous studies found that the H1-50 monoclonal antibody cross-reacts with pancreatic tissue and islet beta-cells, and further investigation revealed that it binds to PHB protein. We screened the binding epitopes using a phage 12-peptide library and identified a specific binding sequence of H1-50 mAb to influenza A virus HA. The study provides new insights into the role of influenza virus in type 1 diabetes.
CURRENT MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Federico Bertoglio, Doris Meier, Nora Langreder, Stephan Steinke, Ulfert Rand, Luca Simonelli, Philip Alexander Heine, Rico Ballmann, Kai-Thomas Schneider, Kristian Daniel Ralph Roth, Maximilian Ruschig, Peggy Riese, Kathrin Eschke, Yeonsu Kim, Dorina Schaeckermann, Mattia Pedotti, Philipp Kuhn, Susanne Zock-Emmenthal, Johannes Woehrle, Normann Kilb, Tobias Herz, Marlies Becker, Martina Grasshoff, Esther Veronika Wenzel, Giulio Russo, Andrea Kroeger, Linda Brunotte, Stephan Ludwig, Viola Fuehner, Stefan Daniel Kraemer, Stefan Duebel, Luca Varani, Gunter Roth, Luka Cicin-Sain, Maren Schubert, Michael Hust
Summary: COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2, which utilizes human ACE2 enzyme as receptor for cell entry. Researchers have used phage display to select anti-SARS-CoV-2 spike antibodies from universal antibody gene libraries, showing potential inhibition of infection.
NATURE COMMUNICATIONS
(2021)
Review
Immunology
Olivier Tshiani Mbaya, Philippe Mukumbayi, Sabue Mulangu
Summary: The unprecedented West Africa Ebola outbreak from 2013-2016 accelerated the development of medical countermeasures against Ebola virus disease. In the recent second-largest Ebola outbreak in the Democratic Republic of the Congo, two IPs, REGN-EB3 and mAb114, showed efficacy compared to the control arm ZMapp in an RCT. The FDA approved both medications, marking a significant advancement in EVD therapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Editorial Material
Microbiology
Nicasio Mancini
Summary: M. P. Doyle, J. R. Genualdi, A. L. Bailey, N. Kose et al. report on the cloning of fully human monoclonal antibodies directed against yellow fever virus (YFV), with mAb YFV-136 showing interesting cross-YFV substrain-neutralizing features. These antibodies hold significance not only for potential clinical use but also for enhancing our understanding of YFV biology and developing effective therapeutic and preventive strategies for YFV and other flaviviruses.
Article
Immunology
Timm Weber, Julian Potthoff, Sven Bizu, Maurice Labuhn, Leona Dold, Till Schoofs, Marcel Horning, Meryem S. Ercanoglu, Christoph Kreer, Lutz Gieselmann, Kanika Vanshylla, Bettina Langhans, Hanna Janicki, Luisa J. Stroeh, Elena Knops, Dirk Nierhoff, Ulrich Spengler, Rolf Kaiser, Pamela J. Bjorkman, Thomas Krey, Dorothea Bankwitz, Nico Pfeifer, Thomas Pietschmann, Andrew Flyak, Florian Klein
Summary: The study identified a small percentage of HCV-infected individuals who produced highly effective HCV-neutralizing antibodies with exceptional potency and breadth. Analysis of antibody structures and genetic mutations revealed the importance of specific gene segments and mutation sites for high neutralizing activity.
Article
Virology
Toshitada Takemori, Akiko Sugimoto-Ishige, Hironori Nishitsuji, Yushi Futamura, Michishige Harada, Tomomi Kimura-Someya, Takehisa Matsumoto, Teruki Honma, Miho Tanaka, Masami Yaguchi, Kyoichi Isono, Haruhiko Koseki, Hiroyuki Osada, Daiki Miki, Takashi Saito, Takashi Tanaka, Takehiro Fukami, Toshio Goto, Mikako Shirouzu, Kunitada Shimotohno, Kazuaki Chayama
Summary: Entry inhibitors are being developed to enhance the treatment of HBV patients with oral nucleoside/nucleotide analogues (NA). Therapeutic MAbs with neutralizing activity against HBs antigens have been developed to amplify the effectiveness of NA therapy. Targeting NTCP can provide an effective treatment option for patients with chronic hepatitis B, regardless of viral genotype, mutations, and the presence of subviral particles.
JOURNAL OF VIROLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sebastien Viollet, Elise Enouf, Justine Picot, Lena Noel, Simon Huet, Deborah Le Pennec, Thomas Secher, Nathalie Heuze-Vourc'h, Olivier Kitten, Mathieu Cinier
Summary: Nanofitins are small and hyperthermostable protein scaffolds that can be used for topical therapeutics, particularly for pulmonary infectious diseases. They have been developed as highly specific anti-SARS-CoV-2 molecules that show therapeutic efficacy both in vitro and in vivo. The multimeric construction of different Nanofitins seems to involve a conformation switch of the spike trimer, and the tetrameric construction remains stable and active after nebulization.
Article
Immunology
Brian M. Petersen, Sophia A. Ulmer, Emily R. Rhodes, Matias F. Gutierrez-Gonzalez, Brandon J. Dekosky, Kayla G. Sprenger, Timothy A. Whitehead
Summary: Monoclonal antibodies are key therapeutics for cancer, inflammation, and infectious diseases. Natural human antibody repertoire-based scoring matrices can accurately predict mutations in therapeutic antibodies. High frequency mutations in natural human antibody repertoires have the potential to improve existing therapeutic antibodies and reduce immunogenicity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Ryan J. Malonis, James T. Earnest, Arthur S. Kim, Matthew Angeliadis, Frederick W. Holtsberg, M. Javad Aman, Rohit K. Jangra, Kartik Chandran, Johanna P. Daily, Michael S. Diamond, Margaret Kielian, Jonathan R. Lai
Summary: This study isolated and characterized MAYV-reactive human monoclonal antibodies from a CHIKV-convalescent donor, revealing five mAbs that can potently neutralize multiple alphaviruses. Two of these broadly neutralizing mAbs provided protection against musculoskeletal disease induced by CHIKV and MAYV in mice.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Doina Atanasiu, Wan Ting Saw, Tina M. Cairns, Roselyn J. Eisenberg, Gary H. Cohen
Summary: The study reveals that herpes simplex virus entry and cell-cell fusion rely on specific glycoproteins, with virus-neutralizing antibodies playing a crucial role in blocking these processes. By combining different antibodies, the research aims to determine their impact on the complex, providing insights for the design of effective therapeutic approaches.
JOURNAL OF VIROLOGY
(2021)
Review
Biochemistry & Molecular Biology
Alessandro Rocca, Carlotta Biagi, Sara Scarpini, Arianna Dondi, Silvia Vandini, Luca Pierantoni, Marcello Lanari
Summary: Respiratory syncytial virus (RSV) is the main cause of acute respiratory tract infections in children worldwide, and while only symptomatic treatment is available, passive immune prevention methods are being developed for all infants. The high cost of passive immunoprophylaxis limits its prescription to high-risk children, though most hospitalized RSV-infected children do not fully meet the criteria for immune prophylaxis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)