Article
Chemistry, Analytical
Ruben Pawellek, Ulrike Holzgrabe
Summary: Charged aerosol detection (CAD) has been introduced into European Pharmacopoeia (Ph. Eur.) monographs for assessing impurities of vigabatrin, facilitated by ion pair chromatography (IPC) using tridecafluoroheptanoic acid (TDFHA) as an ion-pairing reagent. However, high levels of background noise from the ion-pairing reagent have impaired UV-CAD detection. By evaluating the mobile phase composition's influence on background noise using response surface methodology, an alternative method using mixed-mode chromatography (MMC) was developed, enabling better selectivity and shorter analysis time. The MMC method shows improved LOQs, reduced instrumentation needs, and halved sample concentration compared to IPC.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Applied
M. R. Infantes-Garcia, S. H. E. Verkempinck, J. M. Guevara-Zambrano, M. E. Hendrickx, T. Grauwet
Summary: The developed HPLC-CAD method allows for simultaneous quantification of multiple lipid digestion products, providing an interesting analytical platform for studying the molecular mechanism of lipid digestion.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Food Science & Technology
Aneta Slawinska, Ewa Jablonska-Rys, Anna Stachniuk
Summary: The refractive index detector lacks sensitivity, reproducibility, and is susceptible to interference. This study introduced a new method using HPLC-CAD for identifying and quantifying free sugars in mushrooms. The developed method showed good linearity and reproducibility, successfully detecting fructose, mannitol, and glucose in mushroom samples, with mannitol being the most abundant sugar in white Agaricus bisporus and trehalose in Pleurotus ostreatus.
FOOD ANALYTICAL METHODS
(2021)
Article
Food Science & Technology
Kanta Sato, Tetsushi Yamamoto, Kuniko Mitamura, Atsushi Taga
Summary: By utilizing an analytical method based on charged aerosol detection (CAD), fructosyl oligosaccharides, including 1-kestose and nystose, were successfully identified and analyzed in maple syrup. This method is simple, reliable, and capable of analyzing up to hepta-saccharides within 30 minutes with high validation values. The discovery of a fructosyl oligosaccharide called neokestose in maple syrup sheds light on its saccharide profile.
Article
Biochemical Research Methods
Imad A. Haidar Ahmad, Andrei Blasko, Heather Wang, Tian Lu, Ian Mangion, Erik L. Regalado
Summary: The use of quantitative LC-CAD for poor UV absorbing analytes in multicomponent mixtures has grown exponentially in recent years. Optimizing the power function value (PFV) can lead to a more linear detection signal with higher signal-to-noise ratio and lower relative standard deviation. Different regression models and interpolation/extrapolation processes can be used for LC-CAD assay development, with first-order regression via interpolation being a safe choice for highly regulated laboratories and log-log regression with extrapolation enabling high-throughput assays.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Mengjun Xie, Yueting Yu, Ziyu Zhu, Liping Deng, Bo Ren, Mei Zhang
Summary: The study aimed to establish a method for determining six components in the Bushen Huoxue prescription and to increase the use of CAD in traditional Chinese medicine compounds. The results showed that the method had high accuracy, good repeatability, and simple operation, providing references for improving the quality standard of the Bushen Huoxue prescription.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
Ruben Pawellek, Tibor Muellner, Paul Gamache, Ulrike Holzgrabe
Summary: Charged aerosol detection (CAD) is a universal technique increasingly used for drug quality control in liquid chromatography, but its response is inherently nonlinear. One effective approach to linearize the detector's signal output is to utilize the instrument's built-in power function value (PFV) setting. Two independent methods for determining the optimal PFV significantly improve the accuracy of the method in predicting the optimal PFV for each analyte based on experimental results.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Multidisciplinary
Ruben Pawellek, Jovana Krmar, Adrian Leistner, Nevena Djajic, Biljana Otasevic, Ana Protic, Ulrike Holzgrabe
Summary: The study accurately predicted the CAD response of homologous fatty acids under varying experimental conditions using a GBT model, showing high predictive ability.
JOURNAL OF CHEMINFORMATICS
(2021)
Article
Biochemical Research Methods
Jiarui Gao, Fan Wang, Bingqi Zhu, Ping Li, Zhijian Wang, Jian Wang
Summary: A sensitive and direct method using high performance liquid chromatography coupled with diode array detector and charged aerosol detector (HPLC-DAD-CAD) was developed for the analysis of impurities in quinolone antibiotics. The method achieved good separation and accurate detection of impurities in quinolone antibiotics through optimizing chromatographic conditions.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Materials Science, Multidisciplinary
Yiheng Ping, Lei Lei, Peng Qiao, Jun Zheng, Shixu Tao, Yi Cheng, Hao Mao, Hongping Ma, Shiqing Xu
Summary: The study demonstrates the X-ray activated UV afterglow in LiLuF4: Ce microcrystals, with the presence of strong scintillation emissions originating from Ce3+ ions. It is found that a slight excess of LiF content in the precursors solution is crucial for achieving pure LiLuF4: Ce microcrystals. The results also indicate that LiLuF4 matrix is more suitable for UV scintillation and afterglow from Ce3+ activators compared to NaLuF4 and NaGdF4 hosts.
Article
Biochemistry & Molecular Biology
Magdalena Roslon, Malgorzata Jaworska, Elzbieta L. Anuszewska
Summary: A method using high-performance liquid chromatography with a charged aerosol detector method (HPLC-CAD) was developed for the separation and determination of phospholipids from cell membranes. The method achieved improved recovery of the tested phospholipids through an improved lipid isolation procedure and ultrasound-assisted extraction. The method showed good performance in terms of analytical range, precision, intermediate precision, and accuracy, and can be used to assess metabolic changes between different types of cells.
Article
Chemistry, Analytical
Carolin Langer, Regine Suess
Summary: A simple and time-saving HPLC method was developed for simultaneous analysis of hydrophobic drugs and conventional liposomal components, with wide calibration range and excellent precision achieved.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
P. Lezana, M. F. Garcia-Mayoral, B. Lamothe, M. Pena-Abaurrea
Summary: This study describes a simultaneous mixed-mode HPLC method combined with a universal and non-selective-response detector for the complete ethoxymer profiling of alcohol ethoxy sulphate mixtures. The developed methodology provides extensive information regarding the detailed individual ethoxymer profile of AES formulations, showing promising applications in surfactant industry for specific synthesis routes and detergency properties. The method shows excellent correlation with NMR experiments, as well as high precision and linearity along the working concentration range, indicating its potential for routine surfactant screenings.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Astronomy & Astrophysics
S. R. Kulkarni
Summary: The diffuse far-ultraviolet (FUV) background has been a subject of interest to astronomers since the 1970s. It plays a central role in the physics of diffuse atomic phases. The high latitude diffuse FUV emission is believed to have three components: stellar FUV reflected by dust grains, FUV from other galaxies and the intergalactic medium, and an unknown offset component. This paper investigates the production of FUV photons by conventional sources and concludes that most, if not all, of the offset component can be explained by the radiation from these sources.
PUBLICATIONS OF THE ASTRONOMICAL SOCIETY OF THE PACIFIC
(2022)
Article
Engineering, Electrical & Electronic
Jiyunting Sun, Pepijn Veefkind, Peter van Velthoven, Pieternel F. Levelt
Summary: This study found a strong correlation between AERONET AAOD and satellite retrieved UVAI, and developed a numerical relation by DNN to predict global AAOD and SSA. The DNN predicted aerosol absorption is satisfying for samples with AOD larger than 0.1, and it performs better for smaller absorbing aerosols.
IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING
(2021)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)