Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1178, Issue 1-2, Pages 145-153Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2007.11.071
Keywords
hemoglobin; lysozyme; glucose-6-phospate dehydrogenase; poly(ethylene glycol); sodium poly(acrylic acid); aqueous two-phase system; liquid - liquid extraction
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The partition of hemoglobin, lysozyme and glucose-6-phospate dehydrogenase (G6PDH) in a novel inexpensive aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The effect of NaCl and Na2SO4, pH and PEG molecular size on the partitioning has been studied. At high pH (above 9), hemoglobin partitions strongly to the PEG-phase. Although some precipitation of hemoglobin occurs, high recovery values are obtained particularly for lysozyme and G6PDH. The partitioning forces are dominated by the hydrophobic and electrochemical (salt) effects, since the positively charged lysozyme and negatively charged G6PDH partitions to the non-charged PEG and the strongly negatively charged polyacrylate enriched phase, respectively. (c) 2007 Elsevier B.V. All rights reserved.
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