Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1212, Issue 1-2, Pages 82-88Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2008.10.036
Keywords
Erythropoietin; Recombinant human EPO; Lectin affinity; Wheat germ agglutinin; Lateral flow test
Funding
- Phadia AB, Uppsala, Sweden
- Vinnova, Swedish Agency for Innovation system
Ask authors/readers for more resources
This work exploits the combination of the lectin affinity chromatography (LAC) with all ultra-sensitive immunochromatographic assay to differentiate several types of erythropoietin (EPC). The chromatographic behaviours of different commercial types of recombinant human EPO (rhEPO), EPO analogues (Aranesp) and urine human EPO (uhEPO) from healthy individuals on eight lectin-Sepharose columns, have been worked out. Results show that when using wheat germ agglutinin (WGA)-Sepharose columns, a careful desorption regime starting with very low concentration (2mM) of the competitive sugar N-acetylglucosamine (GlcNAc) makes it possible to efficiently distinguish endogenous EPO from recombinant EPO and EPO analogues. (C) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available