Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1180, Issue 1-2, Pages 179-186Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2007.11.110
Keywords
pharmaceutical analysis; linezolid; chiral purity
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A method for the enantioseparation of linezolid, the first compound of a truly new class of antibiotics-the oxazolidinones, was developed. The elaborated method of linezolid enantiomers separation was successfully performed using an anionic single-isomer cyclodextrin-heptakis-(2,3-diacetyl-6-sulfato)-beta-cyclodextrin (HDAS-beta-CD) as a resolving agent with the help of the charged resolving agent migration model (CHARM model). The best results were obtained with 27.5 mM HDAS-beta-CD dissolved in 50 mM borate buffer, pH 9.0, 15 degrees C, normal polarity. The facile strategies for the reversal of the enantiomers elution order are also described. Afterwards, the optimized method was validated in terms of sensitivity, linearity, accuracy and precision. (c) 2007 Elsevier B.V. All rights reserved.
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