Journal
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
Volume 85, Issue 10, Pages 1419-1422Publisher
WILEY
DOI: 10.1002/jctb.2433
Keywords
recombinant rhamnosidase; Clostridium stercorarium; citrus peel waste; naringin; rhamnose
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Funding
- CSIR [38(1133)/07/EMR-II]
- CSIR, New Delhi
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The citrus fruit processing industry generates substantial quantities of waste rich in glycosylated phenolic substances such as naringin, which are a valuable natural source of polyphenols as well as L-rhamnopyranose. Naringin is the major polyphenol in bitter orange peel and its hydrolysis by alpha-L-rhamnosidase (EC 3.2.1.40) catalyzes the cleavage of the terminal rhamnosyl groups to form prunin and rhamnose. In this work, a recombinant alpha-L-rhamnosidase from C. stercorarium was shown to be suitable for narigin hydrolysis. The recombinant rhamnosidase was found to be relatively stable at 60 degrees C, and a residual activity close to 50% after 180 min of incubation was demonstrated. The purified enzyme established hydrolysis of naringin extracted from citrus peel waste (CPW). The result indicated that recombinant alpha-L-rhamnosidase has industrial applicability and is an interesting candidate for producing rhamnose from citrus peel. (C) 2010 Society of Chemical Industry
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