Article
Engineering, Environmental
Kaiyue Huo, Yujie Liu, Rui Huang, Yiting Zhang, Honglu Liu, You Che, Chao Yang
Summary: A novel promoter engineering-based strategy was developed to create efficient PNP-mineralizing bacteria by introducing a complete PNP biodegradation pathway from Pseudomonas sp. strain WBC-3 into the genome of a biosafety and soil-dwelling bacterium Pseudomonas putida KT2440. Five strong promoters were identified from P. putida KT2440 and independently inserted into the genome to create a mutant strain with the highest PNP degradation rate and strong tolerance against high concentrations of PNP, demonstrating complete and efficient PNP mineralization. This strategy opens new avenues for creating efficient degraders for bioremediation.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Engineering, Chemical
Yen-Hui Lin, Bing-Han Ho
Summary: The biodegradation of phenol, sodium salicylate, and 4-chlorophenol by Pseudomonas putida was studied. Experimental results showed that Pseudomonas putida had a faster degradation rate for sodium salicylate compared to phenol, and its degradation rate was less sensitive to substrate inhibition. In the binary substrate system, sodium salicylate had a higher competitive inhibition on phenol biodegradation. In the ternary substrate system, the complete degradation of phenol and sodium salicylate required 14 and 11.5 days respectively, and a 90% removal efficiency for 4-chlorophenol was achieved within 14 days. The experimental results of the chemostat system agreed with the model prediction.
Article
Environmental Sciences
Yuxin Zhao, You Che, Fang Zhang, Jiacheng Wang, Weixia Gao, Tong Zhang, Chao Yang
Summary: In this study, an efficient pathway construction strategy for integrating synthetic biodegradation pathways into the chromosome of Pseudomonas putida KT2440 was developed. The strategy resulted in improved chromosomal integration efficiencies and highlighted the potential for in situ bioremediation. Additionally, the strategy can be expanded to a broad range of bacterial species for widespread applications in bioremediation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Saba Miri, Seyyed Mohammadreza Davoodi, Mohammad Hossein Karimi Darvanjooghi, Satinder Kaur Brar, Tarek Rouissi, Richard Martel
Summary: The study investigated the co-metabolic biodegradation of p-xylene by Pseudomonas putida in the presence of Benzene, Toluene, and Ethylbenzene. The GMDH model was proposed to predict the transformation of non-growth substrates in the presence of multiple growth substrates, showing potential for accurately predicting the degradation process in co-metabolic systems.
PROCESS BIOCHEMISTRY
(2021)
Article
Environmental Sciences
Kaiyue Huo, Siqi Wang, Wanwan Zhao, Hongfu Guo, Weini Xiong, Ruihua Liu, Chao Yang
Summary: In this study, the first engineered DCA-mineralizing strain KTU-P8DCA was constructed by enhancing pathway expression with a strong promoter P8 in the biosafety strain Pseudomonas putida KT2440. Strain KTU-P8DCA exhibited superior tolerance to high concentrations of DCA and showed excellent genetic stability in continuous passage culture. This research has significant implications for the bioremediation of sites heavily contaminated with DCA.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Mourad Fedeila, Zohra Hachaichi-Sadouk, Luis Fernando Bautista, Raquel Simarro
Summary: Two bacterial strains, Pseudomonas aeruginosa and Pseudomonas putida, isolated from industrial effluents in El-Harrach, Algeria, were able to degrade clopidogrel bisulfate under aerobic conditions. The consortium showed high efficiency in removing clopi-dogrel bisulfate at concentrations ranging from 5 to 1500 mg/L within 96 hours. The study also demonstrated the influence of pH, temperature, and supplemental sources of carbon and nitrogen on the biodegradation of clopidogrel bisulfate.
JOURNAL OF CONTAMINANT HYDROLOGY
(2023)
Article
Agricultural Engineering
Shoushuai Feng, Liangqi Gong, Yanke Zhang, Yanjun Tong, Hailing Zhang, Deqiang Zhu, Xing Huang, Hailin Yang
Summary: Bioaugmentation using a bacterial consortium of Pseudomonas putida SW-3 and Rhodococcus ruber SS-4 successfully degraded benzene, toluene, and styrene pollutants with an efficiency of up to 97.9%. The balanced bacterial community structure established after intervention led to an increase in the abundance of BTS-degrading bacteria, enhancing the overall degradation capability of the bacterial community.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Liang Wang, Zhongce Hu, Mian Hu, Jun Zhao, Peijie Zhou, Yongjie Zhang, Xin Zheng, Yifeng Zhang, Zhong-Ting Hu, Zhiyan Pan
Summary: This study demonstrates that the photosynthetic bacteria driven cometabolic biodegradation system associated with exogenous carbon sources is an effective microbial technique for treating 4-chlorophenol. The research also proposes a new plausible biodegradation pathway for 4-chlorophenol in the cometabolic system based on the detection of cyclohexanone generation. The study provides new insights into the application of cometabolic technology for chlorophenol treatment in industrial hypersaline wastewater.
BIORESOURCE TECHNOLOGY
(2022)
Article
Infectious Diseases
Ana Candela, Manuel J. Arroyo, Maria Sanchez-Cueto, Mercedes Marin, Emilia Cercenado, Gema Mendez, Patricia Munoz, Luis Mancera, David Rodriguez-Temporal, Belen Rodriguez-Sanchez
Summary: The study evaluated matrix-assisted laser desorption ionization-iime of flight mass spectrometry (MALDI-TOF MS) and Fourier-transform infrared spectroscopy (FTIR-S) as diagnostic alternatives for the detection of Pseudomonas aeruginosa sequence type (ST) 175 isolates involved in a hospital outbreak. The results showed that the combination of these two technologies, along with machine learning tools, can effectively monitor high-risk clones and isolates in nosocomial outbreaks.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Engineering, Environmental
Han Lin, Wenqi Guan, Yibo Xiao, Yujiao Qu, Yuan Ren
Summary: This study investigates the effects of polymerized ferric sulfate (PFS), fulvic acid (FA), and extracellular microalgal metabolites (EMA) on stimulating 2-chlorophenol (2-CP) degradation by Acinetobacter sp. strain ZY1 and elucidates the underlying mechanisms. The addition of these biostimulants increased 2-CP degradation efficiency and restored ZY1 morphology under 2-CP stress. Transcriptomic analysis showed upregulation of genes related to membrane composition, transmembrane transport, metabolism, and cell proliferation, leading to enhanced 2-CP degradation.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Ali Assadi, Roya Alimoradzadeh, Hossein Movahedyan, Mohammad Mehdi Amin
Summary: This study investigated the microbial and biodegradation kinetics of acclimated-mixed sludge to 4-chlorophenol in an aerobic sequencing batch reactor. The biodegradation kinetics were predicted accurately using the Haldane formulae. However, pure strains isolated from mixed sludge did not show higher 4-CP-removal efficiency due to the presence of an intermediate meta-cleavage product.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Multidisciplinary Sciences
Saurabh Gangola, Samiksha Joshi, Saurabh Kumar, Barkha Sharma, Anita Sharma
Summary: The study identified a bacterial isolate 2D with high tolerance and effective biodegradation rate for multiple pesticides. Analysis showed the highest similarity of 2D with Bacillus cereus, and the study on laccase under pesticide stress conditions suggested significantly increased enzyme production.
Article
Agronomy
Stefania Fontanazza, Alessia Restuccia, Giovanni Mauromicale, Aurelio Scavo, Cristina Abbate
Summary: The study aimed to identify microorganisms responsible for degrading BP mulch films through a combination of biodegradation and molecular tests. A field trial revealed a higher abundance of Pseudomonas putida in BP plots, showing potential for accelerating the degradation of BP mulch films and reducing plastic pollution in agriculture.
Article
Engineering, Environmental
Panlin Wang, Jindi Zhao, Yongqiang Ruan, Xinyi Cai, Jin Li, Lihui Zhang, He Huang
Summary: Two strains of Pseudomonas aeruginosa were able to survive with polypropylene as the sole source of carbon, leading to weight loss and changes in the surface structure of PP.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Environmental Sciences
Carlos Costa, Anais Santos, Milena A. Vega
Summary: This study investigated the kinetics of biodegradation of Arab Light crude oil by two oleophilic strains (Bacillus licheniformis and Pseudomonas putida). The results showed that at an initial crude oil concentration of 8.6 g/L and a temperature of 30 °C, the degradation process followed first-order kinetics with degradation rates of 705 mg/Ld and 843 mg/Ld for P. putida and B. licheniformis, respectively. These findings are important for utilizing microorganisms in bioremediation of petroleum alkane contamination.
Editorial Material
Environmental Sciences
Bin Cao, April Z. Gu, Pei-Ying Hong, Renata Ivanek, Baikun Li, Aijie Wang, JingYi Wu
ENVIRONMENTAL RESEARCH
(2021)
Article
Thermodynamics
Jingxin Zhang, Danning Gu, Jiaqi Chen, Yiliang He, Yanjun Dai, Kai-Chee Loh, Yen Wah Tong
Summary: The study examines the efficiency of using biogas generated from anaerobic digestion of food waste for electricity and heat generation, as well as the energy balance after scaling up the system. Through system optimization, significant improvements in thermal and electrical energy outputs can be achieved, enabling the efficient utilization of waste resources and provision of green energy for communities.
ENERGY CONVERSION AND MANAGEMENT
(2021)
Article
Green & Sustainable Science & Technology
Joseph Jegan Roy, Srinivasan Madhavi, Bin Cao
Summary: Spent lithium-ion batteries containing toxic metals and electrolytes can be extracted and reused through bioleaching to reduce environmental risks. This study successfully recovered cobalt and lithium using Acidithiobacillus ferrooxidans in high-density bioleaching, with over 90% cobalt extraction confirmed.
JOURNAL OF CLEANER PRODUCTION
(2021)
Review
Environmental Sciences
Shan Wu, Yichao Wu, Bin Cao, Qiaoyun Huang, Peng Cai
Summary: The potential role of soil biofilms in the spread of ARGs is discussed in this review, aiming to expand the limited knowledge about ARGs in the soil microenvironment. Techniques such as microfluidic platforms and high-throughput sequencing are also mentioned as tools for studying soil biofilms and transfer of ARGs.
CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sakcham Bairoliya, Jonas Koh Zhi Xiang, Bin Cao
Summary: The article summarizes key findings on eDNA in environmental samples and discusses methods to extract and quantify eDNA, as well as the importance of considering eDNA in experimental results interpretation. The authors suggest factors to consider when designing experiments and analyzing data to account for the contribution of eDNA to environmental DNA-based community analyses.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Guang-Lei Ma, Hartono Candra, Li Mei Pang, Juan Xiong, Yichen Ding, Hoa Thi Tran, Zhen Jie Low, Hong Ye, Min Liu, Jie Zheng, Mingliang Fang, Bin Cao, Zhao-Xun Liang
Summary: Naturally occurring hydrazones are rare, but widely used in the synthesis of organic compounds and functional materials. In this study, a novel family of microbial metabolites, tasikamides, was discovered. These tasikamides have a unique cyclic pentapeptide scaffold and contain a hydrazone group. The biosynthesis of tasikamides was found to require two gene clusters, one for the cyclic peptide scaffold and another for the synthesis of alkyl 5-hydroxylanthranilate. The findings revealed a novel mechanism for the formation of specialized metabolites through the coupling of two biosynthetic pathways.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Minh Phuong Do, Joseph Jegan Roy, Bin Cao, Madhavi Srinivasan
Summary: The study successfully regenerated important materials from end-of-life lithium-ion batteries through bioleaching, offering a new solution to address the global issue of electronic waste.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Environmental Sciences
Ming-Juan Cui, Aloysius Teng, Jian Chu, Bin Cao
Summary: This study presented a quantitative, high-throughput urease activity assay based on a 96-well plate, which can be used for comparing and rapidly screening urease-producing bacteria. The assay revealed bacterial strains with high urease activity and showed the inhibitory effect of calcium on urease activity. The method has potential wide applications in environmental, civil, and medical research.
ENVIRONMENTAL RESEARCH
(2022)
Article
Engineering, Environmental
Sakcham Bairoliya, Apoorva Goel, Manisha Mukherjee, Jonas Koh Zhi Xiang, Bin Cao
Summary: Monochloramine disinfection of drinking water leads to the release of extracellular DNA and RNA, which may impact microbiome analysis and pose health concerns.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Engineering, Environmental
Yi-Nan Liu, Sha Deng, Sakcham Bairoliya, Bin Cao
Summary: In the past two decades, the concept of microbial resources has evolved from conserved strains and genomic data to functional microbial consortia, superior phenotypes, biochemical and signaling systems, efficient enzymes, secondary metabolites, and genetic traits. However, the mining of microbial resources from water is severely underexplored compared to chemical and energy resources. This review summarizes various approaches used to mine microbial resources from water, discusses their advantages and limitations, and explores future directions in this field.
RESOURCES CONSERVATION AND RECYCLING
(2023)
Article
Chemistry, Multidisciplinary
Joseph Jegan Roy, Ernest Jun Jie Tang, Minh Phuong Do, Bin Cao, Madhavi Srinivasan
Summary: Research on recycling electrode materials, particularly anode graphite, from spent lithium-ion batteries has gained attention due to economic benefits and environmental concerns. This study successfully recycled anode graphite from bioleaching residue, achieving a purity of 99.78% and demonstrating excellent electrochemical performance.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Environmental Sciences
Jonas Koh, Sakcham Bairoliya, Maria Salta, Zin Thida Cho, Jenny Fong, Mei Lin Neo, Simon Cragg, Bin Cao
Summary: In this study, plastic debris collected from various coastal ecosystems around Singapore were found to be predominantly influenced by sediment rather than the plastic materials. The plastisphere communities on the plastic debris included potential plastic degraders, bacteria involved in biogeochemical cycles, hygienically relevant bacteria, and potential pathogens. This study highlights the urgent need to understand the fate and ecological impacts of plastic debris accumulated in coastal habitats.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Agricultural Engineering
Jiachen Sun, Le Zhang, Kai-Chee Loh
Summary: The pursuit of affordable biofuels requires continuous improvement of utilization strategies, focusing on cost-effective raw materials, accessible bioprocessing, and high-quality products. This study proposes an integrated approach combining synergistic alkali pretreatment and arrested AD, exploring the interaction between initial pH, cell viability/functionality, and VFA production. The results demonstrate the feasibility of combining pretreatment and methanogenesis arrest for cost-effective and scalable biofuels production.
BIORESOURCE TECHNOLOGY
(2023)
Article
Microbiology
Amit Kumar, Daphne H. P. Ng, Sakcham Bairoliya, Bin Cao
Summary: In densely populated cities with limited land, storing surface water in underground spaces is a potential solution to meet the rising demand for clean water. Additionally, the deployment of floating solar photovoltaic (PV) systems over water has increased due to the imperative need for renewable solar energy and limited land resources. However, the influence of the absence of sunlight on microbial community function and water quality is largely unknown. This study aimed to reveal microbial processes and water quality dynamics in surface water stored in the dark.
MICROBIOLOGY SPECTRUM
(2022)
Article
Engineering, Environmental
Sakcham Bairoliya, Apoorva Goel, Manisha Mukherjee, Jonas Koh Zhi Xiang, Bin Cao
Summary: Monochloramine induces the release of DNA and RNA in drinking water, with sequencing of the eDNA able to assemble the whole genome of the model organism, while the eRNA contains non-coding RNA and mRNA, potentially serving as signaling molecules in environmental systems.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
