Journal
JOURNAL OF CHEMICAL NEUROANATOMY
Volume 61-62, Issue -, Pages 176-188Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchemneu.2014.10.001
Keywords
Paired associates learning; Spatial memory; Microglial morphology; Dentate gyrus; Cebus apella
Categories
Funding
- Fundacao de Amparo a Pesquisa do Para-FADESP
- Pro-Reitoria de Pesquisa e Pos-Graduacao da Universidade Federal do Para Brazilian Research Council-CNPq [307749/2004-5, 471077/2007-0, EDITAL 02/2014-PIAPA]
- FINEP, Instituto Brasileiro de Neurociencias-IBNnet
Ask authors/readers for more resources
We investigated whether the morphology of microglia in the molecular layer of the dentate gyrus (DG-Mol) or in the lacunosum molecular layer of CA1 (CA1-LMol) was correlated with spatial learning and memory in the capuchin monkey (Cebus apella). Learning and memory was tested in 4 monkeys with visuo-spatial, paired associated learning (PAL) tasks from the Cambridge battery of neuropsychological tests. After testing, monkeys were sacrificed, and hippocampi were sectioned. We specifically immunolabeled microglia with an antibody against the adapter binding, ionized calcium protein. Microglia were selected from the middle and outer thirds of the DG-Mol (n = 268) and the CA1-LMol (n = 185) for three-dimensional reconstructions created with Neurolucida and Neuroexplorer software. Cluster and discriminant analyses, based on microglial morphometric parameters, identified two major morphological microglia phenotypes (types I and II) found in both the CA1-LMol and DG-Mol of all individuals. Compared to type II, type I microglia were significantly smaller, thinner, more tortuous and ramified, and less complex (lower fractal dimensions). PAL performance was both linearly and non-linearly correlated with type I microglial morphological features from the rostral and caudal DG-Mol, but not with microglia from the CA1-LMol. These differences in microglial morphology and correlations with PAL performance were consistent with previous proposals of hippocampal regional contributions for spatial learning and memory. Our results suggested that at least two morphological microglial phenotypes provided distinct physiological roles to learning-associated activity in the rostral and caudal DG-Mol of the monkey brain. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available