Journal
JOURNAL OF CEREAL SCIENCE
Volume 58, Issue 3, Pages 472-478Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jcs.2013.09.009
Keywords
Wheat; alpha-Amylase; Rht; 1BS/1RS
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Funding
- Harper Adams University
- Home-Grown Cereals Authority
- UK Biotechnology and Biological Sciences Research Council
- Biotechnology and Biological Sciences Research Council [BB/D007348/1, BBS/E/C/00005202, BB/D019001/1] Funding Source: researchfish
- BBSRC [BB/D007348/1, BB/D019001/1, BBS/E/C/00005202] Funding Source: UKRI
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To study the role of abscisic acid (ABA) and gibberellin (GA) sensitivity in regulating pre-maturity alpha-amylase (PMA) in wheat grains, plants were grown in a glasshouse under cold-shock and ambient conditions. alpha-amylase activity in response to applied ABA and GA was measured in detached-grains with the embryo removed (in vitro) and in intact-grains attached to the plant (in situ). The in vitro experiment was conducted using Spark (low PMA-susceptible genotype) and Rialto (highly PMA-susceptible genotype), with the aim of defining the time point for GA-sensitivity. The results showed an increase in GA-sensitivity at about 640 degree days after anthesis (DAA) in Rialto. There was no evidence for a change in ABA-sensitivity in either variety. The in situ experiments were conducted using genotypes from a Spark x Rialto doubled haploid population segregating for the Rht-D1a (tall) or Rht-D1b allele and for the presence or absence of 1BS/1RS. For Rht-D1a (tall) or Rht-D1b genotypes with or without 1BS/1RS, the cold-shock significantly increased GA-sensitivity, whereas there was no significant change in ABA-sensitivity. These results show PMA is related to an increase in GA-sensitivity that occurs in the aleurone at around 640 degree DAA, and can be enhanced by environmental factors (e.g. cold-shock). (C) 2013 Elsevier Ltd. All rights reserved.
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