Article
Biochemistry & Molecular Biology
Nguyen-Tuong Ho, Shu-Wei Lin, Yi-Rong Lee, Chii-Ruey Tzeng, Shu-Huei Kao
Summary: OPNb and OPNc are overexpressed in endometriosis and adenomyosis, and CD44v and the epithelial-mesenchymal transition (EMT) process are also upregulated. Overexpression of OPNb and OPNc splice variants leads to morphological changes, actin remodeling, cell proliferation, cell migration, and EMT through binding with CD44 and alpha v beta 3, subsequently activating the PI3K and NF-kappa B pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Fiona L. Cousins, Ronald Pandoy, Shiying Jin, Caroline E. Gargett
Summary: This review discusses the discovery of human and mouse endometrial epithelial stem/progenitor cells, their markers and location, as well as their roles in endometrial dynamics, including regeneration and re-epithelialization during the menstrual cycle. Additionally, it outlines the techniques used to identify these cells and their potential role in endometrial proliferative disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Michael P. Vu, Catherine Cheng
Summary: The eye lens plays a crucial role in focusing light onto the retina, while cataracts are a major cause of blindness worldwide. Recent studies have shown the importance of Eph-ephrin bidirectional signaling in maintaining lens transparency. This study investigated the expression of Eph receptors and ephrin ligands in different cell fractions of the lens, revealing the presence of multiple receptor-ligand pairs that may contribute to lens homeostasis.
Article
Genetics & Heredity
Mojtaba Najafi, Yongzhi Guo, Goran Andersson, Patrice Humblot, Erik Bongcam-Rudloff
Summary: LPS, a component of Gram-negative bacteria outer membrane, is involved in the pathogenic processes of mastitis and metritis in dairy cattle. In vitro studies have shown that LPS stimulates the proliferation of bovine endometrial epithelial cells. Transcriptomic analysis of these cells treated with LPS revealed differentially expressed genes associated with proliferation and apoptosis. The analysis also identified putative transcription factors and their interactions, providing insights into the mechanisms underlying the proliferative and apoptosis processes. Additionally, the study shed light on the early signaling and metabolic pathways involved in the immune response and cell proliferation in bovine endometrial epithelial cells infected with Escherichia coli-LPS.
Article
Reproductive Biology
Xiaoxiao Gao, Xiaodan Li, Zhibo Wang, Kang Li, Yaxu Liang, Xiaolei Yao, Guomin Zhang, Feng Wang
Summary: Supplementing L-arginine (L-Arg) promotes the growth of endometrial ductal gland invaginations (DGI) and alleviates oxidative stress in nutrient-restricted sheep. In vitro, L-Arg reduces reactive oxygen species (ROS) release, enhances antioxidant enzyme activity, improves cell viability, and alleviates cell cycle arrest and apoptosis caused by oxidative stress.
Article
Food Science & Technology
Qianhong Ye, Haoyu Li, Baoyang Xu, Ziyi He, Xianghua Yan
Summary: The study finds that butyrate improves endometrial epithelial cell receptivity by inhibiting cell proliferation, increasing pinocytosis activity on the cell surface, and enhancing adhesiveness to porcine trophoblast cells. Butyrate also increases prostaglandin synthesis and significantly affects purine metabolism, pyrimidine metabolism, and the FoxO signaling pathway. The H3K9ac/FoxO1/PCNA pathway is shown to contribute to the effects of cell proliferation inhibition and uterine receptivity improvement induced by butyrate through siRNA and ChIP-seq experiments.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Veterinary Sciences
Luying Cui, Yang Qu, Hele Cai, Heng Wang, Junsheng Dong, Jun Li, Chen Qian, Jianji Li
Summary: Meloxicam has been used to relieve pain and control inflammation in cows with metritis and endometritis. The study found that meloxicam inhibited cell cycle progression and reduced cell proliferation in lipopolysaccharide-stimulated BEEC, possibly mediated by Wnt/beta-catenin and PI3K/AKT pathways.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Medicine, General & Internal
Christos Masaoutis, Natalia Georgantzoglou, Panagiotis Sarantis, Irene Theochari, Nikolaos Tsoukalas, Mattheos Bobos, Paraskevi Alexandrou, Alexandros Pergaris, Dimitra Rontogianni, Stamatios Theocharis
Summary: The study found that Eph receptors are expressed differently in thymic epithelial tumors, with correlations to tumor subtypes and Masaoka stage.
Article
Reproductive Biology
Shijie Lyu, Yaying Zhai, Xiaoting Zhu, Qiaoting Shi, Fuying Chen, Geyang Zhang, Zijing Zhang, Eryao Wang
Summary: Embryonic mortality is a major factor contributing to reduced conception rates in the cattle industry. Insufficient endometrial receptivity is a key factor causing embryo implantation failure and losses. The study found that bta-miR-200b can influence the apoptosis of bovine endometrial epithelial cells by targeting the MYB gene.
Article
Biochemistry & Molecular Biology
Katsuaki Ieguchi, Takeshi Tomita, Toshifumi Takao, Tsutomu Omori, Taishi Mishima, Isao Shimizu, Massimiliano Tognolini, Alessio Lodola, Takuya Tsunoda, Shinichi Kobayashi, Satoshi Wada, Yoshiro Maru
Summary: The study revealed that tumor-derived ephrin-A1 plays a significant role in a worse prognosis, with ADAM-mediated cleavage of ephrin-A1 being a key factor in lung metastasis. Inhibition of cleavage or antagonization of receptors can greatly reduce lung metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yoshiko Hashimoto, Tomoko Tsuzuki-Nakao, Naoko Kida, Yoshiyuki Matsuo, Tetsuo Maruyama, Hidetaka Okada, Kiichi Hirota
Summary: The endometrium undergoes repeated changes during the menstrual cycle, and chronic endometritis can lead to implantation failure and miscarriage. In this study, the effects of inflammatory cytokines, hypoxia-inducible factor (HIF), and inflammation on endometrial epithelial cells were investigated. The results showed that pro-inflammatory factors and hypoxia activated HIF-1 and promoted epithelial-mesenchymal transition (EMT) in endometrial epithelial cells.
Article
Food Science & Technology
Chang-qian Fang, Yuan Teng, Yi-ting Wang, Yuan-yuan Zhao, Xian Zheng, Lan Long, Jian Zhang, Ren-dong Zheng, Xiao-long Cao, Cui-hua Jiang
Summary: This study identified arjunolic acid (AA) as a selective inhibitor of glucagon secretion from the Cyclocarya paliurus leaves. AA not only improved hyperglycemia and hyperglucagonemia in T2DM mice, but also enhanced pancreatic islet morphology and function. The selective inhibition of AA on glucagon secretion was regulated by the interaction between ephrin-A1 and EphA4, subsequently activating the PI3K and Akt pathway.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Cell & Tissue Engineering
Siyu Xia, Ming Wu, Xinhao Zhou, Xiu Zhang, Lina Ye, Kang Zhang, Yiyi Kang, Jun Liu, Yunci Zhang, Wang Wu, Dirong Dong, Hong Chen, Hui Li
Summary: Conditionally reprogrammed physiological endometrial epithelial cells provide a novel therapeutic strategy for IUA by repairing the injured endometrium and improving pregnancy rate.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Endocrinology & Metabolism
Fang Ren, Yizi Wang, Peng Chen, Cuishan Guo
Summary: The downregulation of SPOCK2 was found to promote cell proliferation, adhesion, and invasion in human endometrial epithelial cells, suggesting its involvement in the pathogenesis and progression of endometriosis, as well as its malignant transformation.
GYNECOLOGICAL ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Rachelle Mendoza, Nayanendu Saha, Amir Momeni, Elmer Gabutan, Mouyed Alawad, Amir Dehghani, John Diks, Bo Lin, Donghai Wang, Mohamed Alshal, William Fyke, Bingcheng Wang, Juha P. Himanen, Prem Premsrirut, Dimitar B. Nikolov
Summary: Research has shown that serum levels of ephrin-A1 and ADAM12 are significantly elevated in COVID-19 patients and correlate with disease progression, indicating potential targets for therapeutic development. Conversely, TNF alpha and ADAM17 levels were elevated in a small subset of patients, suggesting a different inflammatory pathway in COVID-19 progression.
Review
Biochemistry & Molecular Biology
Gregory A. Johnson, Heewon Seo, Fuller W. Bazer, Guoyao Wu, Avery C. Kramer, Bryan A. McLendon, Joe W. Cain
Summary: Conceptus elongation and early placentation require energy expenditure, increased glucose, fructose, and amino acids in the uterine lumen, and coordination of multiple metabolic pathways for conceptus development.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Katherine M. Halloran, Claire Stenhouse, Robyn M. Moses, Avery C. Kramer, Nirvay Sah, Heewon Seo, Simon G. Lamarre, Gregory A. Johnson, Guoyao Wu, Fuller W. Bazer
Summary: This study demonstrates the importance of one carbon metabolism and serinogenesis in ovine conceptuses during the peri-implantation period of pregnancy. It also reveals that glucose and fructose are the preferred substrates for generating formate required for nucleotide synthesis.
Article
Engineering, Environmental
Nayoung Min, Hahyun Park, Taeyeon Hong, Garam An, Gwonhwa Song, Whasun Lim
Summary: This study evaluated the developmental toxicity of the herbicide prometryn in zebrafish embryos, revealing that it shortened body length, induced organ development defects, reactive oxygen species accumulation, and cell death. It was also found to interfere with mitochondrial function and energy metabolism.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Endocrinology & Metabolism
Sunwoo Park, Jiyeon Ham, Changwon Yang, Wonhyoung Park, Hahyun Park, Garam An, Jisoo Song, Taeyeon Hong, Soo Jin Park, Hee Seung Kim, Gwonhwa Song, Whasun Lim
Summary: This study found that melatonin and dienogest can effectively reduce surgically induced endometriotic lesions. Melatonin functions by inhibiting cell proliferation, inducing apoptosis, and regulating calcium homeostasis. Additionally, it affects mitochondrial function and ATP production, suppresses signaling pathways, and inhibits cell migration as a treatment for endometriosis.
JOURNAL OF PINEAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Hojun Lee, Garam An, Whasun Lim, Gwonhwa Song
Summary: Tebufenpyrad, a pyrazole acaricide and insecticide, has been widely used in several countries for various crops. This study investigated the biochemical and physiological effects of tebufenpyrad on porcine trophectoderm and luminal epithelial cells, revealing its antiproliferative effects, apoptosis induction, reactive oxygen species production, cell cycle arrest, disrupted calcium homeostasis, altered MAPK signaling pathways, reduced cell migration ability, and influence on gene expression related to pregnancy.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Garam An, Miji Kim, Junho Park, Hahyun Park, Taeyeon Hong, Whasun Lim, Gwonhwa Song
Summary: Chloroxylenol is a widely used anti-microbial compound, and recent studies have emphasized the need to assess its potential for ecotoxicity due to increased usage during the coronavirus pandemic and ban on other antimicrobial ingredients. Although the detrimental effect of chloroxylenol on zebrafish viability has been reported, the mechanisms underlying its toxicity are not well understood. This study used the zebrafish model to investigate the responses to chloroxylenol and predict its toxicity to human health and ecology. The findings suggest the risks of chloroxylenol exposure and highlight the importance of further ecotoxicological studies.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Environmental Sciences
Junho Park, Garam An, Hahyun Park, Taeyeon Hong, Whasun Lim, Gwonhwa Song
Summary: This study demonstrated the developmental toxicity of thiabendazole, a benzimidazole fungicide, using zebrafish as a representative toxicological model. It showed various morphological malformations, as well as triggered apoptosis, reactive oxygen species production, and inflammatory response in zebrafish larvae exposed to thiabendazole. The study also revealed the significant changes in PI3K/Akt and MAPK signaling pathways, as well as toxicity in various organs, such as cardiovascular, neurotoxicity, and hepatic and pancreatic toxicity.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Medicine, Research & Experimental
Xilong Li, Fuller W. Bazer, Gregory A. Johnson, Robert C. Burghardt, Guoyao Wu
Summary: This study used gilts as an animal model to investigate the effects of dietary supplementation with L-citrulline (Cit) on placental angiogenesis and embryonic survival. Compared to the control group, Cit supplementation significantly increased the number of viable fetuses, placental blood vessels, placental weight, and fluid volumes. Additionally, Cit supplementation enhanced the synthesis of NO and polyamines, as well as the expression of angiogenic factors and aquaporins in the placenta. Overall, dietary Cit supplementation improved conceptus development and survival by enhancing placental NO and polyamine synthesis and promoting angiogenesis.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Article
Food Science & Technology
Garam An, Junho Park, Whasun Lim, Gwonhwa Song
Summary: This study aimed to investigate the harmful effects of folpet on bovine mammary glands and milk production using MAC-T cells. The results showed that folpet exhibited cytotoxicity against MAC-T cells, causing apoptosis and cell death. Furthermore, folpet treatment induced oxidative stress and activated MAPK signaling pathways. This is the first report highlighting the detrimental impacts of folpet on bovine mammary glands and the dairy industry, providing insights into the intracellular mechanisms using MAC-T cells.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Food Science & Technology
Jiyeon Ham, Jisoo Song, Gwonhwa Song, Whasun Lim
Summary: Through experiments on porcine uterine epithelial cells and trophectoderm cells, we found that Oryzalin can inhibit cell growth and proliferation, and also affect the cell cycle progression. In addition, it can induce apoptosis and disrupt mitochondrial membrane polarity. We also confirmed that Oryzalin can decrease adenosine triphosphate (ATP) production and increase reactive oxygen species (ROS) generation in cells. In summary, Oryzalin causes implantation failure through ROS-mediated cellular signaling regulation and inhibition of migration potential.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Reproductive Biology
Wonhyoung Park, Junho Park, Sunwoo Park, Whasun Lim, Gwonhwa Song
Summary: This study found that picolinafen has a significant impact on the viability of porcine trophectoderm and luminal epithelial cells involved in the implantation process during early pregnancy. It increased apoptosis, disrupted mitochondrial function, and caused intracellular ROS accumulation, which led to a reduction in calcium levels. Furthermore, it inhibited the migration of porcine trophectoderm cells. These effects were mediated by the activation of the MAPK and PI3K signaling pathways.
Article
Reproductive Biology
Makenzie G. Newton, Claire Stenhouse, Katherine M. Halloran, Nirvay Sah, Robyn M. Moses, Wenliang He, Guoyao Wu, Fuller W. Bazer
Summary: The synthesis and secretion of polyamines in the uterus of ewes are regulated by changes in progesterone, estradiol, and their receptors. These hormones and receptors play important roles in the reproductive performance of mammals by affecting the secretion and transport of nutrients in the uterine lumen.
BIOLOGY OF REPRODUCTION
(2023)
Article
Reproductive Biology
Claire Stenhouse, Katherine M. Halloran, Makenzie G. Newton, Robyn M. Moses, Nirvay Sah, Larry J. Suva, Dana Gaddy, Fuller W. Bazer
Summary: Tissue-nonspecific alkaline phosphatase (TNSALP) plays a critical role in phosphate transport and homeostasis at the maternal-conceptus interface in ruminants.
BIOLOGY OF REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Miji Kim, Taeyeon Hong, Garam An, Whasun Lim, Gwonhwa Song
Summary: This study demonstrated the developmental toxicity and mechanism of action of the herbicide benfluralin using zebrafish as a model. Benfluralin caused morphological and physiological alterations, increased reactive oxygen species (ROS) levels, induced heart and vascular structure malformations, and inhibited liver development. It also affected normal growth by abnormal expression of cell cycle regulatory genes and increased oxidative stress, inflammation, and apoptosis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Garam An, Junho Park, Whasun Lim, Gwonhwa Song
Summary: In this study, we investigated the hepatotoxicity and mechanism of action of pyridaben using zebrafish and ZFL cells. Pyridaben caused liver size reduction and apoptosis, and these effects were associated with uncontrolled intracellular calcium flow and downregulation of the PI3K/Akt signaling cascade.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)