Article
Biochemistry & Molecular Biology
Lin Leilei, Sun Xue, Li Yan, Luo Yuyuan, Wang Ying, Qiu Wenke, Yu Xuesong, Li Ming
Summary: Recent studies have shown that TGF-beta 1 secreted from hepatocytes exposed to PM2.5 may lead to activation of hepatic stellate cells, potentially contributing to liver fibrosis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Nutrition & Dietetics
You-Jung Hong, Gil-Hwan Kim, Yongdo Park, Hye-Jin Jo, Min-Woo Nam, Dong-Gu Kim, Hwangeui Cho, Hyun-Joo Shim, Jong-Sik Jin, Hyunsoo Rho, Chang-Yeob Han
Summary: This study demonstrates that Suaeda glauca extract (SGE) has therapeutic effects on liver fibrosis. SGE supplementation reduces liver fibrosis induced by CCl4, decreases HSC activation and inflammation, and improves blood biochemical parameters. The anti-fibrotic effects of SGE are mediated through the inhibition of TGF beta 1-Smad2/3 signaling in HSCs.
Article
Pharmacology & Pharmacy
Na Jiang, Jing Zhang, Jian Ping, Lieming Xu
Summary: This article investigates the role of salvianolic acid B (Sal B) in autophagy of hepatic stellate cells (HSCs) in liver fibrosis. The study shows that TGF-beta 1 induces autophagosome formation and flux in HSCs, while Sal B inhibits autophagy and activation of HSCs by suppressing autophagosome formation and flux. Furthermore, Sal B inhibits TGF-beta 1-induced autophagy and activation of HSCs by downregulating the MAPK signaling pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Ting-Ting Li, Xiao-Wei Su, Lin-Lin Chen, Wan-Nian Zhang, Jun-Ping Zhang, Yan Wang, Wei-Heng Xu
Summary: Hepatic fibrosis is a stage of chronic liver disease that can lead to cirrhosis or liver cancer. The activation of hepatic stellate cells (HSCs) is a crucial event in fibrosis development and inhibiting this activation has been shown to alleviate fibrosis. Roxarsone, an organoarsenic additive used in livestock production, has been found to inhibit HSC activation and improve liver function in a mouse model of fibrosis. These findings suggest that Roxarsone could be a potential treatment for liver fibrosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cell & Tissue Engineering
Fei Yin, Li-Cui Mao, Qi-Qi Cai, Wen-Hua Jiang
Summary: Studies have shown that HGF-transfected hUCMSCs can decrease cell viability of HSCs-T6, promote apoptosis, inhibit activation, and reduce the expression of Collagen I, Collagen III, TGF-beta 1, Smad2, and Smad3, possibly through inhibiting the TGF-beta 1/Smads signaling pathway. These findings suggest that HGF-transfected hUCMSCs may be a novel therapeutic approach for treating LF.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Maximilian Schinagl, Tamara Tomin, Juergen Gindlhuber, Sophie Honeder, Raphael Pfleger, Matthias Schittmayer, Michael Trauner, Ruth Birner-Gruenberger
Summary: This study investigated the activation of hepatic stellate cells (HSC) through an increase in growth medium serum concentration, revealing cellular processes involved in HSC transformation. Activated HSC showed increased production of ribosomal proteins and proteins related to migration and cell cycle control, along with a decrease in cholesterol and fatty acid biosynthesis proteins. The findings provide insights into HSC activation characteristics and present a accessible model for studying HSC activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Parasitology
Ping Huang, Huihui Ma, Yun Cao, Tingzheng Zhan, Tingting Zhang, Xinyi Wang, Yanan Zhang, Jing Xu, Chaoming Xia
Summary: This study demonstrates the crucial role of hepatic stellate cells (HSCs) regulated by the TGF-beta 1/Smad signaling pathway in liver fibrosis during Schistosoma japonicum infection.
PARASITES & VECTORS
(2022)
Article
Gastroenterology & Hepatology
Shiwei Wang, Lingling He, Fan Xiao, Meixin Gao, Herui Wei, Junru Yang, Yang Shu, Fuyang Zhang, Xiaohui Ye, Ping Li, Xiaohua Hao, Xingang Zhou, Hongshan Wei
Summary: GLT25D1 is upregulated in hepatic stellate cells (HSCs) during liver fibrosis and promotes the progression of fibrosis by affecting HSC activation and collagen stability.
JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY
(2023)
Article
Gastroenterology & Hepatology
Taixiong Xue, Lin Yue, Guonian Zhu, Zui Tan, Hongyao Liu, Cailing Gan, Chen Fan, Xingping Su, Yuting Xie, Tinghong Ye
Summary: In this study, a phenylacrylic acid derivative, FA11, was synthesized and found to have excellent antifibrotic activity, making it a potential candidate for the treatment of liver fibrosis.
LIVER INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Minako Sakurai, Peter Weber, Gretchen Wolff, Annika Wieder, Julia Szendroedi, Stephan Herzig, Bilgen Ekim uestuenel
Summary: Non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), and liver fibrosis are progressive liver diseases associated with metabolic syndrome. The elevated expression of TSC22D4 in patients with type 2 diabetes, NAFLD, and NASH suggests its involvement in the progression of these diseases. The study reveals that TSC22D4 contributes to the activation, proliferation, and migration of hepatic stellate cells (HSCs), implicating it as a key regulator in the development of liver fibrosis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Zuoning Han, Yanling Ma, Gary Cao, Zhengping Ma, Ruihua Chen, Mary Ellen Cvijic, Dong Cheng
Summary: The study found that αV01 integrin plays a role in non-canonical TGF-0 signaling pathways and that αV01 inhibition may be an effective strategy for the treatment of liver fibrosis.
BIOCHEMICAL JOURNAL
(2021)
Article
Pathology
Hayato Urushima, Hideto Yuasa, Tsutomu Matsubara, Noriyuki Kuroda, Yaiko Hara, Kouji Inoue, Kenjiro Wake, Tetsuji Sato, Scott L. Friedman, Kazuo Ikeda
Summary: This study revealed the activation mechanism of HSCs, identifying the relationship between adherens junctions and HSC activation, recognizing the crucial role of E-cadherin in the activation of hepatic stellate cells, and discovering the critical role of TAZ-mediated mechanotransduction in HSC activation.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Soonjae Hwang, Sangbin Park, Uzma Yaseen, Ho-Jae Lee, Ji-Young Cha
Summary: Liver fibrosis is a progressive and debilitating disease characterized by excessive deposition of extracellular matrix proteins. Our study reveals that KLF10 acts as a negative regulator of TGF-beta signaling pathway, suppressing hepatic stellate cell activation and fibrogenesis through modulation of ATF3 expression. Targeting the KLF10-ATF3 axis may have therapeutic implications for liver fibrosis treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Min Shen, Yujia Li, Yingqian Wang, Jiangjuan Shao, Feng Zhang, Guoping Yin, Anping Chen, Zili Zhang, Shizhong Zheng
Summary: The study reveals that under conditions of ferroptosis, the total levels of m(6)A modification are significantly increased, potentially triggering autophagy activation by stabilizing BECN1 mRNA. YTHDF1, identified as a key m(6)A reader protein, promotes BECN1 mRNA stability and autophagy activation, making it a potential target for HSC ferroptosis.
Article
Biochemistry & Molecular Biology
Mengzhen Kuang, Hao Wu, Lan Hu, Xinying Guo, Daochuan He, Bo Liu, Mengqian Chen, Jie Gu, Jianxin Gu, Xiaoqing Zeng, Yuanyuan Ruan
Summary: The up-regulation of FUT8 was found to inhibit TGF-beta 1-induced HSC activation in a negative feedback loop, suggesting a potential new therapeutic strategy for liver fibrosis by targeting FUT8.
GLYCOCONJUGATE JOURNAL
(2021)
Article
Engineering, Biomedical
Yao Teng, Zixuan Zhao, Farah Tasnim, Xiaozhong Huang, Hanry Yu
Summary: This study introduces a new drug testing platform, SteatoChip, which improves the formation and function of HepaRG organoids by in situ differentiation and perfusion in a uniform size distribution, showing enhanced hepatic differentiation and improved functionality, potentially accelerating drug development for nonalcoholic fatty liver disease (NAFLD).
Article
Engineering, Biomedical
Zhihua Li, Zijian Chen, Yanan Gao, Yi Xing, Yuping Zhou, Yucheng Luo, Weihong Xu, Zhengchang Chen, Xu Gao, Kapish Gupta, Karthic Anbalakan, Lei Chen, Chuang Liu, Jian Kong, Hwa Liang Leo, Chengzhi Hu, Hanry Yu, Qiongyu Guo
Summary: A new type of magnetic micro-anchor, SM(2)A, has been developed for magnetic guided endovascular localization. It demonstrates stable anchoring in the desired site and produces the necessary action. This micro-anchor can be a promising platform for precision treatment and diagnosis.
Article
Engineering, Biomedical
Yucheng Luo, Yutao Ma, Zijian Chen, Yanan Gao, Yuping Zhou, Xiaoya Liu, Xuezhe Liu, Xu Gao, Zhihua Li, Chuang Liu, Hwa Liang Leo, Hanry Yu, Qiongyu Guo
Summary: Particulate embolic agents with calibrated sizes have gained significant interest in therapeutic embolotherapies. In this study, shape-anisotropic microembolics based on PVA were developed using a simple fabrication method. The embolization behaviors of these nonspherical microembolics were evaluated in vitro and in vivo models. The results demonstrated that compared to microspheres, the elongated microembolics displayed superior endovascular navigation capability, penetration depth, and embolization stability.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Multidisciplinary Sciences
Yi-An Yang, Emmanuelle Nguyen, Gautham Hari Narayana Sankara Narayana, Melina Heuze, Chaoyu Fu, Hanry Yu, Rene-Marc Mege, Benoit Ladoux, Michael P. Sheetz
Summary: E-cadherin is a key molecule involved in mechanotransduction between cells in tissues. Epithelial cells are able to test and respond to the mechanical properties of neighboring cells through contractions of E-cadherin adhesions. This process requires the involvement of multiple proteins.
Article
Chemistry, Analytical
Xiaolin Wu, Cheryl Chan, Stacy L. Springs, Yie Hou Lee, Timothy K. Lu, Hanry Yu
Summary: WS-RADICA is a new nucleic acid detection method that enables rapid, sensitive, and quantitative detection of nucleic acids. It has a lower detection limit and higher inhibitor tolerance, and has been validated for nucleic acids derived from live viruses.
ANALYTICA CHIMICA ACTA
(2022)
Article
Physics, Applied
Yan Kong, Shujian Ong, Mei Hui Liu, Hanry Yu, Dejian Huang
Summary: This study successfully fabricated edible microbeads using food-grade sodium alginate and enhanced their cytoaffinity with gelatin coatings. The modified microbeads have self-fluorescence and can be used as microcarriers for cell culture and meat production.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2022)
Article
Food Science & Technology
Sean Chia, Gavin Teo, Shi Jie Tay, Larry Sai Weng Loo, Corrine Wan, Lyn Chiin Sim, Hanry Yu, Ian Walsh, Kuin Tian Pang
Summary: This study utilized a glycomic approach to profile meat from different species, revealing distinct glycan profiles and significant differences in glycosylation attributes. By analyzing multi-attribute data, meat from different species could be clearly separated, demonstrating the potential of glycomics-based workflows for product authentication in various industries.
Article
Engineering, Environmental
Tao Yang, Dong Li, Yuhua Yan, Fatima-ezzahra Ettoumi, Ricardo A. Wu, Zisheng Luo, Hanry Yu, Xingyu Lin
Summary: In this study, a hydrogel-mediated RT-LAMP method was developed for rapid and accurate quantification of SARS-CoV-2 virus. The technique offers fast, simple, and highly sensitive detection, and can be used for monitoring cold chain foods.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Materials Science, Biomaterials
Yanan Gao, Jingyu Xiao, Zijian Chen, Yutao Ma, Xiaoya Liu, Dishuang Yang, Hwa Liang Leo, Hanry Yu, Jian Kong, Qiongyu Guo
Summary: Developing a three-dimensional in vitro tumor model with vasculature systems for testing endovascular interventional therapies is challenging. Here, an orthotopic liver tumor spheroid model is developed to evaluate transcatheter arterial chemoembolization (TACE) treatment. The model resembles the unique tumor microenvironment and can efficiently assess various diagnostic and therapeutic strategies.
BIOMATERIALS SCIENCE
(2023)
Review
Cell & Tissue Engineering
Kuin Tian Pang, Larry Sai Weng Loo, Sean Chia, Francesca Yi Teng Ong, Hanry Yu, Ian Walsh
Summary: Stem cells have the unique ability to differentiate into specialized cell types, and skeletal muscle stem cells (MuSCs) play important roles in the growth, repair, and regeneration of skeletal muscle tissues. However, the differentiation, proliferation, and expansion processes of MuSCs face significant challenges, and the molecular role of mechanobiology in MuSC growth, proliferation, and differentiation is poorly understood. This review comprehensively summarizes the impact of different mechanical cues on stem cell growth, proliferation, differentiation, and their potential role in disease development.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Multidisciplinary Sciences
Zixuan Zhao, Xinyi Chen, Anna M. Dowbaj, Aleksandra Sljukic, Kaitlin Bratlie, Luda Lin, Eliza Li Shan Fong, Gowri Manohari Balachander, Zhaowei Chen, Alice Soragni, Meritxell Huch, Yi Arial Zeng, Qun Wang, Hanry Yu
Summary: Organoids are simple tissue-engineered cell-based models that mimic the complex structure and function of in vivo tissues. They have a wide range of applications in developmental studies, disease modeling, and personalized medicine. Various engineering strategies have been developed to support their culture, growth, differentiation, and maturation.
NATURE REVIEWS METHODS PRIMERS
(2022)
Article
Multidisciplinary Sciences
N. Jannah M. Nasir, Alberto Corrias, Hans Heemskerk, Eng Tat Ang, Julia H. Jenkins, S. J. Sebastin, Lisa Tucker-Kellogg
Summary: Chronic and recurring pressure ulcers (PUs) require predictive biomarkers. This study suggests that the panniculus carnosus, thought to be vestigial in humans, might play a crucial role in the chronicity and reinjury of heel PUs.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Meeting Abstract
Toxicology
F. Tasnim, X. Huang, Y. T. Soong, H. Yu
TOXICOLOGY LETTERS
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
Nurul Jannah Nasir, Alberto Corrias, Johannes Heemskerk, Eng Tat Ang, Julia Jenkins, Sandeep Sebastin, Lisa Tucker-Kellogg
Article
Cell Biology
Angeline Rouers, Melissa Hui Yen Chng, Bernett Lee, Menaka P. Rajapakse, Kaval Kaur, Ying Xiu Toh, Durgalakshmi Sathiakumar, Thomas Loy, Tun-Linn Thein, Vanessa W. X. Lim, Amit Singhal, Tsin Wen Yeo, Yee-Sin Leo, Kalpit A. Vora, Danilo Casimiro, Bing Lim, Lisa Tucker-Kellogg, Laura Rivino, Evan W. Newell, Katja Fink
Summary: Prior exposure to dengue virus can have varying effects on subsequent infections, with the study showing different cellular responses associated with disease severity in dengue patients.
CELL REPORTS MEDICINE
(2021)