Article
Biotechnology & Applied Microbiology
Souvik Ghosh, Anastasiya Boersch, Shreemoyee Ghosh, Mihaela Zavolan
Summary: Our study explores the effects of Collagen I and Matrigel on the transcriptional profile of cells in a cell culture system. The results indicate a robust transcriptional response to Collagen I in Huh-7 cells, distinct from that induced by Matrigel, potentially aiding in defining appropriate culture and transcription pathways for hepatoma cell lines. This study could help in circumventing bottlenecks of limited availability of primary hepatocytes for preclinical studies of drug targets as systems mimicking the in vivo structure and function of liver cells are still being developed.
Article
Food Science & Technology
Julia Waizenegger, Josephin Glueck, Marcus Henricsson, Claudia Luckert, Albert Braeuning, Stefanie Hessel-Pras
Summary: The study found that PAs downregulate the expression of genes related to bile acid synthesis and transport in human liver cells, potentially leading to progressive impairment of bile acid homeostasis and accumulation of toxic bile acids. Additionally, important biliary efflux mechanisms are disturbed due to PA exposure, which may play a role in the development of severe liver damage in humans intoxicated with PAs.
Article
Cell Biology
Remy Vu, Suoqin Jin, Peng Sun, Daniel Haensel, Quy Hoa Nguyen, Morgan Dragan, Kai Kessenbrock, Qing Nie, Xing Dai
Summary: This study used single-cell RNA sequencing to analyze the cellular and communication changes in young and aged skin wounds. It found that aged skin wounds have a more pronounced inflammatory response and higher abundance of inflammatory/glycolytic macrophages. The study also predicted differences in cell-cell communication between young and aged skin wounds.
Article
Nanoscience & Nanotechnology
Heijun Jeong, Eiyong Park, Sungjoon Lim
Summary: The study introduces a four-dimensional printed memory metasurface for absorbing and reflecting functions within the millimeter-wavelength range, utilizing electromagnetic property memory to achieve the electromagnetic performance of the metasurface. Experimental results demonstrate the potential of this metasurface to significantly contribute to reconfigurable intelligent surfaces and wireless devices.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Physics, Multidisciplinary
Tiago Mendes-Santo, Markus Schmitt, Markus Heyl
Summary: Spectral functions are crucial for bridging experimental probes and theoretical models in condensed matter physics. In this study, we propose a versatile method using neural quantum states to obtain spectral properties through simulations of the dynamics of initially localized excitations in real or momentum space. We apply this method to compute the dynamical structure factor near the quantum critical points of different two-dimensional Ising models, including ones that describe the complex density wave orders of Rydberg atom arrays. By combining it with deep network architectures, our method accurately describes the dynamical structure factors of arrays with up to 24 x 24 spins, including the diverging timescales at critical points.
PHYSICAL REVIEW LETTERS
(2023)
Article
Medicine, Research & Experimental
Katsuyoshi Takata, Lauren C. Chong, Daisuke Ennishi, Tomohiro Aoki, Michael Yu Li, Avinash Thakur, Shannon Healy, Elena Vigano, Tao Dao, Daniel Kwon, Gerben Duns, Julie S. Nielsen, Susana Ben-Neriah, Ethan Tse, Stacy S. Hung, Merrill Boyle, Sung Soo Mun, Christopher M. Bourne, Bruce Woolcock, Adele Telenius, Makoto Kishida, Shinya Rai, Allen W. Zhang, Ali Bashashati, Saeed Saberi, Gianluca D'Antonio, Brad H. Nelson, Sohrab P. Shah, Pamela A. Hoodless, Ari M. Melnick, Randy D. Gascoyne, Joseph M. Connors, David A. Scheinberg, Wendy Beguelin, David W. Scott, Christian Steidl
Summary: PRAME is a key player in the cancer testis antigen family, with deletions in DLBCL being associated with poor outcome, immune escape, and cold tumor microenvironments. Additionally, PRAME downregulation is linked to EZH2 mutations, with PRAME directly interacting with EZH2 as a negative regulator.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Toxicology
Ana Paula Oliveira De Quadros, Bruna Oshiiwa, Marcel Petreanu, Rivaldo Niero, Paulo Cesar Pires Rosa, Alexandra Christine Helena Frankland Sawaya, Mario Sergio Mantovani, Isabel O'Neill De Mascarenhas Gaivao, Edson Luis Maistro
Summary: Rubus rosifolius, a common medicinal plant in southern Brazil, has been found to have genotoxic and apoptotic effects in in vitro studies, indicating potential risks in human use.
TOXICOLOGY IN VITRO
(2023)
Review
Immunology
Ying Fu, Luman Wang, Baichao Yu, Damo Xu, Yiwei Chu
Summary: B-cell-mediated humoral immune response is crucial for protection against infectious diseases. The metabolism of B cells plays a significant role in their homeostasis, development, and function. While the impact of immunometabolism on B cells is not as deeply studied as that on T cells, there is growing evidence suggesting that intracellular metabolism influences B cell functions through transcriptional regulation and signal transduction. This review provides an update on the recent developments in B cell immunometabolism, including its role in development, differentiation, and function in metabolic, autoimmune, and inflammatory disorders.
Article
Biochemical Research Methods
Nicholas Tan Kwan Zen, Kerwin Kwek Zeming, Kim Leng Teo, Mavis Loberas, Jialing Lee, Chin Ren Goh, Da Hou Yang, Steve Oh, James Hui Hoi Po, Simon M. Cool, Han Wei Hou, Jongyoon Han
Summary: The growing interest in regenerative medicine has led to the development of new cell therapies using stem cells. The bone marrow aspirate (BMA) is an important source of mesenchymal stem cells (MSCs), but the conventional method for harvesting MSCs from BMA has limitations. To address these limitations, a scalable microfluidic technology based on deterministic lateral displacement (DLD) has been developed for efficient MSC isolation. The DLD platform allows for fast and effective isolation of MSCs from BMA, improving the recovery rate and reducing processing time.
Article
Biochemical Research Methods
Nicholas Tan Kwan Zen, Kerwin Kwek Zeming, Kim Leng Teo, Mavis Loberas, Jialing Lee, Chin Ren Goh, Da Hou Yang, Steve Oh, James Hui Hoi Po, Simon M. Cool, Han Wei Hou, Jongyoon Han
Summary: The growing interest in regenerative medicine has led to the development of a scalable microfluidic technology called deterministic lateral displacement (DLD) for efficient isolation of mesenchymal stem cells (MSCs) from bone marrow aspirate. This passive, label-free cell sorting method utilizes morphological differences between MSCs and blood cells for effective separation. Compared to centrifugation methods, the multi-chip DLD system achieves a 2-fold increase in MSC recovery with a processing time of 20±5 minutes for 2.5 mL of raw bone marrow aspirate. This technology has the potential to significantly improve the efficiency of downstream cell therapy in clinical settings.
Article
Chemistry, Multidisciplinary
Aixiang Ding, Oju Jeon, David Cleveland, Kaelyn L. Gasvoda, Derrick Wells, Sang Jin Lee, Eben Alsberg
Summary: 4D bioprinting is a promising technique for creating complex cell-laden constructs for tissue/organ regeneration. This study introduces a new single-component micro-flake hydrogel (MFH) system as a bioink for 4D bioprinting. The MFH exhibits scalable production, simple composition, and shear-thinning, shear-yielding, and rapid self-healing properties.
ADVANCED MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Vanessa Naffaa, Isabelle Hochar, Cheryane Lama, Romain Magny, Anne Regazzetti, Pierre Gressens, Olivier Laprevote, Nicolas Auzeil, Anne-Laure Schang
Summary: This study evaluated the effect of bisphenol A (BPA) on oligodendrocyte differentiation by analyzing the cell lipidome. The results suggest that BPA disrupts lipid remodeling during early oligodendrocyte differentiation.
Article
Materials Science, Multidisciplinary
Lei Wang, Mingfeng Liu, Jiangxu Li, Ronghan Li, Hui Ma, Xing-Qiu Chen
Summary: First-principles calculations were used to study the electronic structures, lattice dynamics, electron-phonon coupling, and superconductivity of TBML and TBBL. Both materials were found to be nodal line semimetals, with varying EPC constants and critical temperatures. Applying strain can enhance the superconductivity of TBBL.
Article
Food Science & Technology
Josephin Gluck, Marcus Henricsson, Albert Braeuning, Stefanie Hessel-Pras
Summary: Pyrrolizidine alkaloids (PAs) are secondary plant metabolites found in various plant species and can cause acute intoxication and severe hepatotoxicity in humans. Research on acute human PA intoxication has shown a connection between PA exposure and induction of cholestasis. It was found that PAs have significant structure-dependent effects on bile acid homeostasis, particularly diester type PAs causing the most severe dysregulation of genes associated with cholestasis.
Article
Cell Biology
Madeleine Eichler, Ute Distler, Usman Nasrullah, Aswini Krishnan, Manuel Kaulich, Koraljka Husnjak, Wolfgang Eberhardt, Krishnaraj Rajalingam, Stefan Tenzer, Josef Pfeilschifter, Gergely Imre
Summary: This study uncovers a previously unknown mechanism of caspase-2 tumor suppressor function in human tumor cells, in which selective cleavage of p54nrb disrupts its role in RNA editing, transport, and transcriptional regulation, leading to reduced tumorigenic potential.
CELL DEATH & DISEASE
(2022)
Article
Orthopedics
Ko Matsudaira, Hiroyuki Oka, Yasushi Oshima, Hirotaka Chikuda, Yuki Taniguchi, Yoshitaka Matsubayashi, Mika Kawaguchi, Emiko Sato, Haruka Murano, Thomas Laurent, Sakae Tanaka, Anne F. Mannion
BMC MUSCULOSKELETAL DISORDERS
(2018)
Article
Integrative & Complementary Medicine
Thomas Laurent, Yuji Okuda, Takeshi Chijimatsu, Miki Umeki, Satoru Kobayashi, Yutaro Kataoka, Iwao Tatsuguchi, Satoshi Mochizuki, Hiroaki Oda
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2013)
Article
Infectious Diseases
Hiroyuki Kunishima, Kaoru Ito, Thomas Laurent, Machiko Abe
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2018)
Article
Multidisciplinary Sciences
Daisuke Yamajuku, Takahiko Inagaki, Tomonori Haruma, Shingo Okubo, Yutaro Kataoka, Satoru Kobayashi, Keisuke Ikegami, Thomas Laurent, Tomoko Kojima, Keiji Noutomi, Seiichi Hashimoto, Hiroaki Oda
SCIENTIFIC REPORTS
(2012)
Article
Biology
Thomas Laurent, Yutaro Kataoka, Satoru Kobayashi, Misaki Ando, Seishi Nagamori, Hiroaki Oda