Review
Medicine, General & Internal
Michael. M. M. Gilbert, Samuel. C. C. Mathes, Avinash. S. S. Mahajan, Craig. A. A. Rohan, Jeffrey. B. B. Travers, Anita Thyagarajan
Summary: Sirtuins are a family of proteins that play a role in maintaining the structure and function of the skin, particularly in dermal fibroblasts. Dermal fibroblasts are important in wound healing and maintaining the integrity of the skin, but can undergo cellular senescence as they age. Sirtuins have a potential role in modulating skin conditions and further research is needed for clinical implications in dermatology.
FRONTIERS IN MEDICINE
(2023)
Article
Dermatology
Chie Fujisawa, Makoto Hamanoue, Yayoi Kawano, Daiki Murata, Yuri Akishima-Fukasawa, Tetsuya Okaneya, Takeo Minematsu, Hiromi Sanada, Kayo Tsuburaya, Takuma Isshiki, Tetsuo Mikami, Takehisa Hanawa, Yoshikiyo Akasaka
Summary: miR-146b-5p plays an anti-fibrotic role in skin wounds by suppressing PDGFR alpha and reducing fibrosis. Fibroblastic cells in adipose tissue may pick up and contain miR-146b-5p through exosomes, playing an important role in fibrosis inhibition.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Dermatology
Taihao Quan, Yaping Xiang, Yingchun Liu, Zhaoping Qin, Yan Yang, George Bou-Gharios, John J. Voorhees, Andrzej A. Dlugosz, Gary J. Fisher
Summary: The aging process affects the structure and function of dermal collagen, leading to thinning, fragility, wrinkles, laxity, impaired wound healing, and a microenvironment conducive to cancer. CCN1 protein is elevated in aged human skin, and transgenic mice expressing CCN1 in dermal fibroblasts display accelerated skin dermal aging. This mouse model provides a novel tool for understanding and studying the mechanisms of skin aging.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Developmental Biology
Venkata Thulabandu, Timothy Nehila, James W. Ferguson, Radhika P. Atit
Summary: The study reveals that dermal EZH2 acts as a coordinator in skin development, regulating dermal fibroblast differentiation and Wnt/β-catenin signaling, as well as controlling the initiation of secondary hair follicles. Lack of EZH2 leads to increased epidermal proliferation and differentiation, which can be rescued by inhibiting RA signaling.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Eyleen Corrales, Ella Levit-Zerdoun, Patrick Metzger, Silke Kowar, Manching Ku, Tilman Brummer, Melanie Boerries
Summary: Vemurafenib induces paradoxical changes in human dermal fibroblast (HDF) due to a permissive chromatin landscape, leading to compensatory effects during combination therapies with less specific MAPK/ERK inhibitors. The importance of evaluating the effects of drugs on non-transformed stromal components, especially in combination therapies, is highlighted.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Dermatology
Hiroaki Iwata, Naoya Haga, Hideyuki Ujiie
Summary: Psoriasis is an immune-mediated inflammatory disease characterized by keratinocyte hyperproliferation. Research suggests that in psoriatic lesional skin, dermal fibroblasts overexpress EREG through stimulation with IL-17A and TNF-alpha, contributing to epidermal hyperproliferation.
JOURNAL OF DERMATOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Paula O. Cooper, MaryEllen R. Haas, Satish Kumar R. Noonepalle, Brett A. Shook
Summary: Irregular inflammatory responses contribute to tissue dysfunction and inefficient repair. Skin serves as a powerful model for studying inflammation regulation, particularly in the context of rapid immune responses and subsequent inflammation resolution. Understanding the role of tissue-resident mesenchymal cells in inflammation may lead to new targets for controlling inflammation and promoting repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Mirjana Antonijevic, Despoina Charou, Isbaal Ramos, Maria Valcarcel, Achille Gravanis, Patricia Villace, Noelle Callizot, Marc Since, Patrick Dallemagne, Ioannis Charalampopoulos, Christophe Rochais
Summary: Numerous studies have investigated the role of BDNF and its receptor TrkB in neurodegenerative diseases. BDNF activates the TrkB receptor, promoting neuronal survival and differentiation. In this study, a small data set of compounds was designed and synthesized based on the structure of a previously published activator of TrkB. The compounds were evaluated for their ADME and toxicity profiles, as well as their biological properties and affinity to promote neurite differentiation through the TrkB receptor.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biology
Emanuel Rognoni, Georgina Goss, Toru Hiratsuka, Kalle H. Sipila, Thomas Kirk, Katharina Kober, Prudence PokWai Lui, Victoria Sk Tsang, Nathan J. Hawkshaw, Suzanne M. Pilkington, Inchul Cho, Niwa Ali, Lesley E. Rhodes, Fiona M. Watt
Summary: Solar ultraviolet radiation leads to selective loss of fibroblasts in the skin, with repair predominantly mediated by papillary fibroblast proliferation following acute exposure. Chronic exposure results in permanent loss of papillary fibroblasts, compensated partially by fibroblast membrane protrusion expansion. Loss of papillary fibroblasts is primarily induced by a deregulated inflammatory response, with infiltrating T cells supporting fibroblast survival under UVR-induced environmental stress.
Review
Physiology
Sun Hye Shin, Yoon Hwan Lee, Nark-Kyoung Rho, Kui Young Park
Summary: Skin aging is a complex process involving both internal and external factors. Internal aging is associated with programmed aging and cellular senescence caused by oxidative stress and damage. External aging is a result of environmental factors like UV radiation and pollution, leading to DNA damage and cell dysfunction. Understanding these mechanisms is crucial for developing effective anti-aging treatments.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Suphachai Charoensin, Suwatsak Dansakda
Summary: Oxidative stress triggers changes in cellular energy metabolism and adaptive responses, but the polyphenol antioxidant rosmarinic acid can modulate these effects induced by hydrogen peroxide (H2O2). It reduces reactive oxygen species (ROS) levels, glucose consumption, lactate production, and enhances ATP production and glutathione production through the pentose phosphate pathway. Furthermore, rosmarinic acid downregulates glycolytic enzymes and upregulates antioxidant enzymes, protecting dermal fibroblasts from oxidative damage.
Article
Biochemistry & Molecular Biology
Thanh-Diep Ly, Christopher Lindenkamp, Eva Kara, Vanessa Schmidt, Anika Kleine, Bastian Fischer, Doris Hendig, Cornelius Knabbe, Isabel Faust-Hinse
Summary: This study investigates the impact of inflammation on the expression of XYLT1 and XT-I activity, revealing a previously unknown regulatory mechanism involving components of the inflammasome pathway CTSB and caspase-1. These findings contribute to a better understanding of the inflammatory regulation of XT-I.
Review
Biochemistry & Molecular Biology
Ilja L. Kruglikov, Zhuzhen Zhang, Philipp E. Scherer
Summary: Adipocytes in the superficial layer of subcutaneous adipose tissue undergo cyclic de- and re-differentiation, which play a significant role in the development of skin inflammation under different skin conditions. This inflammation can be attributed to the release of lipids during the de-differentiation phase of the hair follicle cycle, or the widespread release of cathelicidin during the anagen phase. In addition, trans-differentiation of dermal adipocytes into myofibroblasts can contribute to the development of acne.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Hao Wu, Jie Wang, Yue Zhao, Youyou Qin, Xi Chen, Yongting Zhou, Hao Pang, Zidi Xu, Xueyi Liu, Ying Yu, Zhibo Xiao
Summary: Aging is a major cause of degenerative diseases, and photoaging plays an important role in this process. This study explored the mechanisms of skin photoaging and found that UVB irradiation increases chromatin accessibility in human dermal fibroblasts (UVB-HDFs) and leads to changes in gene expression levels. The study also identified differentially expressed miRNAs in extracellular vesicles derived from UVB-HDFs, with miR-22-5p being significantly upregulated and targeting GDF11. Furthermore, GDF11 expression in human skin tissues was found to be strongly associated with age, and overexpression of GDF11 attenuated UVB-induced damage in HDFs. In animal experiments, EVs derived from miR-22-5p-inhibited HDF spheroids improved skin photoaging. These findings provide a novel approach for treating skin photoaging by regulating GDF11 through cell-free methods.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Agriculture, Multidisciplinary
Takashi Ito, Mao Odama, Eiji Maegawa, Kohsuke Suzuki, Yujiro Fujii, Reika Maeda, Shigeru Murakami
Summary: This study investigated the effects of pentacyclic triterpenoids, including betulinic acid (BA), on the cellular proliferation and senescence of normal human dermal fibroblasts (NHDFs). BA treatment increased the proliferation of young fibroblasts and reduced the proportion of senescent cells in high-passage NHDFs. The downregulation of interferon (IFN)-inducible genes, including IFIT1, IFITM1, IFI6, MX1, and OAS2, by BA suggested the suppression of the type I IFN signaling pathway. BA also downregulated the expression of the IRF9 transcriptional factor downstream of the type 1 IFN signaling pathway.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Deepti Bajpai, Spencer Mehdizadeh, Akihiko Uchiyama, Yuta Inoue, Andrew Sawaya, Andrew Overmiller, Stephen R. Brooks, Kowser Hasneen, Meghan Kellett, Elisabetta Palazzo, Sei-ichiro Motegi, Stuart H. Yuspa, Christophe Cataisson, Maria Morasso
Summary: Cutaneous squamous cell carcinoma (cSCC) is the second most common skin cancer, with disruption of keratinocyte proliferation and differentiation playing a role in its development. DLX3, a transcription factor, is found to be associated with higher grade tumors and poor patient survival in cSCC. Studies on mice deficient in Dlx3 show that Dlx3 may function as a tumor suppressor in skin tumorigenesis, with potential therapeutic implications targeting the EGFR-ERBB2 pathway.
Article
Dermatology
Marika Quadri, Antonella Comitato, Elisabetta Palazzo, Natascia Tiso, Andreas Rentsch, Giovanni Pellacani, Alessandra Marconi, Valeria Marigo
Summary: Despite ongoing drug resistance mechanisms in metastatic melanoma, utilizing two polymeric-linked dimeric cGMP analogs as protein kinase G activators demonstrated significant anti-cancer effects, with PA5 showing the most potent inhibitory impact on melanoma cells. By interfering with the EGF pathway, PA5 reduced EGFR phosphorylation and activated, phosphorylated forms of protein kinase B and extracellular signal-regulated kinase 1/2 in melanoma cells, highlighting its potential as a therapeutic strategy for melanoma.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Dermatology
Alessandra Marconi, Marika Quadri, Francesca Farnetani, Silvana Ciardo, Elisabetta Palazzo, Roberta Lotti, Anna Maria Cesinaro, Luca Fabbiani, Cristina Vaschieri, Mario Puviani, Cristina Magnoni, Shaniko Kaleci, Carlo Pincelli, Giovanni Pellacani
Summary: In this study, four distinct melanoma subtypes were identified using reflectance confocal microscopy, each with a specific biomolecular profile and biological behavior associated with tumor aggressiveness.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Review
Medicine, General & Internal
Marika Quadri, Alessandra Marconi, Simran K. Sandhu, Alexi Kiss, Tatiana Efimova, Elisabetta Palazzo
Summary: Cutaneous Squamous Cell Carcinoma (cSCC) is a common type of skin cancer. Various in vitro and in vivo models, including three-dimensional models, have been developed to study its development and invasion. While these models contribute to cancer research, animal models are still needed to address certain issues.
FRONTIERS IN MEDICINE
(2022)
Editorial Material
Medicine, General & Internal
Marika Quadri, Cristina Pellegrini, Tatiana Efimova, Elisabetta Palazzo
FRONTIERS IN MEDICINE
(2022)
Article
Cell & Tissue Engineering
Roberta Lotti, Elisabetta Palazzo, Marika Quadri, Marc Dumas, Sylvianne Schnebert, Diego Biondini, Maria Anastasia Bianchini, Carine Nizard, Carlo Pincelli, Alessandra Marconi
Summary: ETA cells represent the first KSC progenitor with exclusive features, and CD271 identifies and modulates ETA cells, thus participating in the early differentiation and regenerative capacity of the human epidermis.
Article
Oncology
Marika Quadri, Natascia Tiso, Francesco Musmeci, Maria I. I. Morasso, Stephen R. R. Brooks, Luca Reggiani Bonetti, Rossana Panini, Roberta Lotti, Alessandra Marconi, Carlo Pincelli, Elisabetta Palazzo
Summary: This study found differential expression of CD271 in low-risk and high-risk cSCC tumors, and demonstrated that CD271 activity can decrease the proliferation and invasiveness of cSCC, while promoting tumor cell differentiation. CD271 also inhibits the metastatic process of cSCC and increases sensitivity to therapy. Therefore, CD271 may be a promising target for future pharmaceutical development.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Immunology
Roberta Lotti, Jennifer E. Hundt, Ralf J. Ludwig, Brydon Bennett, Antonino Amato, Alessandra Marconi, Carlo Pincelli
Summary: Pemphigus is a life-threatening autoimmune bullous disease that affects the skin and mucous membranes. Current therapies aim to suppress the immune system, but PC111, a fully human monoclonal antibody targeting Fas ligand, has shown promising results in reducing blister formation. In vitro and in vivo experiments have confirmed the mechanism of action and efficacy of PC111 in preventing acantholysis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
