Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 120, Issue 2, Pages 1763-1772Publisher
WILEY
DOI: 10.1002/jcb.27477
Keywords
interleukin-1; miR-181b; nuclear factor-B; osteosarcoma; phosphatase and tensin homolog
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Funding
- Natural Science Foundation of Hunan Province [2017JJ2368, 2018JJ2602]
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So far, microRNA has attracted plenty of interest due to its role in tumorigenesis. Reportedly, miR-181b may be involved in the tumorigenesis of osteosarcoma (OS). In the current study, we attempted to investigate the detailed function and mechanism of miR-181b in OS carcinogenesis. Herein, miR-181a, miR-181b, miR-181c, and miR-181d expressions in OS tissues were higher than that in nontumor tissue samples as examined real-time polymerase chain reaction. Via direct targeting, miR-181b negatively regulated the expression of phosphatase and tensin homolog (PTEN), a well-known tumor suppressor. Furthermore, a small interfering RNA strategy was used to find that interleukin (IL)-1B and nuclear factor-B (NF-B) regulate miR-181b and PTEN expression. Consequently, the repression of PTEN by miR-181b promotes OS cell proliferation. In summary, our data support a critical role for NF-B-dependent upregulation of miR-181b, which further inhibited PTEN expression and promoted the cell proliferation of OS cell lines. The above findings represent a new pathway for the repression of PTEN and the promotion of cell proliferation upon IL-1 induction.
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