Article
Fisheries
Vojtech Kaspar, Martin Hubalek, Martin Psenicka, Katsutoshi Arai, John B. Taggart, Roman Franek
Summary: Methods of induction of uniparental inheritance have been developed for model organisms as well as for species of commercial interest, with cold-shock androgenesis showing promise in successfully inducing haploid progeny in common carp. Compared to other techniques, cold-shock androgenesis was found to be effective in common carp, providing a new possibility for isogenic line production.
Article
Oncology
Mateusz Sikora, Katarzyna Krajewska, Klaudia Marcinkowska, Anna Raciborska, Rafal Jakub Wiglusz, Agnieszka Smieszek
Summary: Osteosarcoma is a malignant bone tumor that mainly affects children and elderly people. The primary treatment methods for osteosarcoma are still underdeveloped, leading to poor survival rates. This study aimed to establish the expression profile of molecular markers related to osteosarcoma survival, progression, and metastasis. The findings may facilitate the selection of reliable cellular models for pre-clinical investigations on osteosarcoma therapy development.
Article
Agriculture, Dairy & Animal Science
Qiaoxin Wang, Xiaohui Li, Hang Sha, Xiangzhong Luo, Guiwei Zou, Hongwei Liang
Summary: Hypoxia stress is a serious issue in fish culture, causing physical and morphological changes, injury, and even death to fish. In this study, differentially expressed miRNAs in silver carp under hypoxia stress were identified through high-throughput sequencing, providing valuable resources for further research on the regulatory mechanism of miRNAs in silver carp under hypoxia stress.
Article
Biochemistry & Molecular Biology
Eun Pyo Kim, Giup Jang, Jin-Wook Kim, Sang Wha Kim, Heaji Chung, Yun Jung Yang, Wan Hee Kim, Geon A. Kim
Summary: Canine mammary gland tumor is commonly diagnosed in intact female dogs. Early and accurate diagnosis is crucial for prognosis. This study analyzed differentially expressed microRNAs and mRNA levels to investigate the malignancy and biopsy locations of the tumors. The miRNA cfa-miR-503 showed altered expression in response to malignancy and could potentially be used as a diagnostic biomarker and anti-tumor drug.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Rongrong Liu, Fei Meng, Xin Li, Hua Li, Guiwen Yang, Shijuan Shan
Summary: This study cloned and characterized common carp STING (CcSTING), and found that it plays a critical role in the immune response against RNA viruses. CcSTING is mainly located in the cytoplasm within the endoplasmic reticulum, and has strong expression in organs such as gills and head kidney. Additionally, overexpression of CcSTING can significantly suppress the replication of SVCV in EPC cells, likely through enhancing IFN and NF-κB activity and up-regulating the phosphorylation of TBK1 and p65.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biology
Qiongxian Yan, Kaijun Wang, Xuefeng Han, Zhiliang Tan
Summary: This study investigated the effects of a high rice-based diet on spleen growth and microRNA expression in goats, showing that the diet promoted the growth of the heart and spleen, as well as regulating the expression of multiple microRNAs and their target genes related to splenocyte proliferation and apoptosis.
Article
Plant Sciences
Abhishek Bohra, Prasad Gandham, Abhishek Rathore, Vivek Thakur, Rachit K. Saxena, S. J. Satheesh Naik, Rajeev K. Varshney, Narendra P. Singh
Summary: The comparative analysis of genome-wide miRNAs and their gene targets between cytoplasmic male sterile (CMS) and fertile lines of pigeonpea suggests a possible role of miRNA-regulated pathways in reproductive development.
Article
Multidisciplinary Sciences
Zhilin Ning, Xinjiang Tan, Yuan Yuan, Ke Huang, Yuwen Pan, Lei Tian, Yan Lu, Xiaoji Wang, Ruicheng Qi, Dongsheng Lu, Yajun Yang, Yaqun Guan, Dolikun Mamatyusupu, Shuhua Xu
Summary: This study analyzed the expression profiles of highly differentiated genes with eastern and western origins in Uyghurs, and found that highly differentiated expression quantitative trait loci (eQTLs) are associated with immunity and metabolism. European-origin alleles tend to be more biasedly expressed, and highly differentiated allele-specific expression (ASE) is enriched in diabetes-associated genes, likely affecting diabetes susceptibility in Uyghurs.
NATIONAL SCIENCE REVIEW
(2023)
Article
Environmental Sciences
Sib Sankar Giri, Min Jung Kim, Sang Guen Kim, Sang Wha Kim, Jeong Woo Kang, Jun Kwon, Sung Bin Lee, Won Joon Jung, Venkatachalam Sukumaran, Se Chang Park
Summary: Curcumin, a compound found in turmeric, has been shown to have antioxidant, anti-inflammatory, and immunostimulatory effects in fish. This study demonstrated that dietary supplementation of curcumin can protect fish against lead-induced toxicity by reducing lead accumulation, improving survival rates, reversing biochemical parameter changes, and enhancing immune responses.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Md Alamgir Kabir, Md. Golam Rabbane, Marco R. Hernandez, Md Aftab Ali Shaikh, Mohammad Moniruzzaman, Xuexiu Chang
Summary: This study investigated the toxicity of high concentrations of cadmium (Cd) on intestinal immunity and microbial diversity in common carp. The results showed that high Cd concentration caused intestinal inflammation, immunosuppression, and changes in the gut microbiome, leading to detrimental effects on the intestinal homeostasis and health status of the fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2024)
Article
Fisheries
Songhuan Chang, Jiali Wang, Chuanju Dong, Yanliang Jiang
Summary: Common carp is an important freshwater farmed fish species worldwide and is susceptible to pathogen Aeromonas hydrophila. In this study, 16S rDNA sequencing was used to analyze the composition and biodiversity of intestinal microbiota in common carp following A. hydrophila infection. The results showed that the diversity and richness of microbiota decreased after infection, and the relative abundance of specific genera was significantly altered. Further analysis revealed potential correlations between certain genera and host resistance against pathogens. This study contributes to our understanding of the underlying mechanisms of intestinal microbial community in resisting pathogens and may provide strategies for disease control in aquaculture.
AQUACULTURE REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Valentina K. Todorova, Stephanie D. Byrum, Samuel G. Mackintosh, Azemat Jamshidi-Parsian, Allen J. Gies, Charity L. Washam, Samir V. Jenkins, Timothy Spiva, Emily Bowman, Nathan S. Reyna, Robert J. Griffin, Issam Makhoul
Summary: This study characterized the microRNA and proteins released in vitro from HBV-related HCC cell lines and normal hepatocyte cell lines using microRNA sequencing and mass spectrometry. The dysregulation of these molecules in exosomes from HCC cells was associated with pathways related to HBV, tumor activity, exosome formation, and protein binding. The results provide insight into the role of HBV infection in HCC development and potential targets for treatment and predictive biomarkers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Ingrid Jakobsen, Max Sundkvist, Niclas Bjorn, Henrik Green, Kourosh Lotfi
Summary: This pilot study explored the treatment-induced expression patterns in AML patients and identified the activation of pathways associated with NF-kappa B signaling, cell cycle progression, oncogenesis, and anti-apoptotic behavior. Furthermore, the significant upregulation of cytidine deaminase, which plays a key role in the deamination of Ara-C, was found in patients who did not achieve complete remission. This study provides a potential method for elucidating new drug targets and biomarkers for evaluating treatment response in AML patients.
Article
Biotechnology & Applied Microbiology
Tianning Chen, Kailun Huo, Dece Kong, Shan Su, Tieyi Yang, Weiwei Zhang, Jin Shao
Summary: By studying peripheral blood samples from early postmenopausal women, we identified key lncRNAs that are associated with the progression of postmenopausal osteoporosis. One of the candidates, MIR22HG, was found to potentially serve as a miRNA precursor and regulate the FoxO signaling pathway in bone metabolism. These findings are of great significance for predicting and diagnosing early postmenopausal osteoporosis (PMOP).
Article
Environmental Sciences
Seyyed Morteza Hoseini, Kave Khosraviani, Fatemeh Hosseinpour Delavar, Mohammad Arghideh, Fatemeh Zavvar, Seyed Hossein Hoseinifar, Hien Van Doan, Erfan Zabihi, Miriam Reverter
Summary: This study investigated the combined effects of copper and polyvinyl chloride (PVC) microparticles on metal accumulation, histopathological biomarkers, and targeted transcriptomics in Cyprinus carpio liver. The presence of PVC microparticles in water facilitated hepatic copper accumulation. All treatments induced significant hepatic stress and inflammation, but transcriptional responses related to detoxification pathways and apoptotic mechanisms were mixed and often down-regulated in fish exposed to copper and/or PVC microparticles. Exposure to copper and/or PVC microparticles resulted in hyperemia, leukocyte infiltration, and an increase in melanomacrophage centers number and area. Generally, the severity of the lesions followed the order: PVC microparticles < copper < copper+ PVC microparticles. In conclusion, PVC microparticles act as a copper vector, facilitating copper accumulation in the fish liver and increasing tissue damage.
MARINE POLLUTION BULLETIN
(2022)