Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 113, Issue 12, Pages 3843-3852Publisher
WILEY
DOI: 10.1002/jcb.24298
Keywords
ZEBRAFISH; SAP30L; TRANSCRIPTION; MICROARRAY; HEMATOPOIESIS; CARDIOGENESIS
Categories
Funding
- Biocenter Finland
- Tampere Tuberculosis Foundation
- Emil Aaltonen Foundation
- Academy of Finland Research Council for Health [115260, 121003, 1286223, 135980, 135736, 139225]
- Foundation for Paediatric Research in Finland
- Finnish Medical Foundation
- Competitive Research Funding of Tampere University Hospital [9J062, 9K073, 9M052, 9L070, 9K093, 9L075, 9M080, 9M093]
- Marie Curie International Reintegration Grant within the 7th European Community Framework Programme
- Sigrid Juselius Foundation
- Nona and Kullervo Vare Foundation
- Paivikki and Sakari Sohlberg Foundation
- Academy of Finland (AKA) [135736, 121003, 115260, 115260, 135736, 121003, 139225, 139225] Funding Source: Academy of Finland (AKA)
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The Sin3A-associated proteins SAP30 and SAP30L share 70% sequence identity and are part of the multiprotein Sin3A corepressor complex. They participate in gene repression events by linking members of the complex and stabilizing interactions among the protein members as well as between proteins and DNA. While most organisms have both SAP30 and SAP30L, the zebrafish is exceptional because it only has SAP30L. Here we demonstrate that SAP30L is expressed ubiquitously in embryonic and adult zebrafish tissues. Knockdown of SAP30L using morpholino-mediated technology resulted in a morphant phenotype manifesting as cardiac insufficiency and defective hemoglobinization of red blood cells. A microarray analysis of gene expression in SAP30L morphant embryos revealed changes in the expression of genes involved in regulation of transcription, TGF-beta signaling, Wnt-family transcription factors, and nuclear genes encoding mitochondrial proteins. The expression of the heart-specific nkx2.5 gene was markedly down-regulated in SAP30L morphants, and the cardiac phenotype could be partially rescued by nkx2.5 mRNA. In addition, changes were detected in the expression of genes known to be important in hemoglobin synthesis and erythropoiesis. Our results demonstrate that SAP30L regulates several transcriptional pathways in zebrafish embryos and is involved in the development of cardiac and hematopoietic systems. J. Cell. Biochem. 113: 38433852, 2012. (C) 2012 Wiley Periodicals, Inc.
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