Article
Biochemistry & Molecular Biology
Fateme Eidizade, Mohammad Soukhtanloo, Rahele Zhiani, Jamshid Mehrzad, Farshad Mirzavi
Summary: This research investigates the effects of urolithin-B on glioblastoma multiforme (GBM) U87 cells. The results suggest that urolithin-B can decrease cell viability, induce necrosis and cell cycle arrest, increase reactive oxygen species production, and reduce cell migration in U87 cells. These findings indicate that urolithin-B may be a promising compound for GBM treatment.
Article
Cell Biology
Mathilde Kerherve, Sara Rosinska, Kilian Trillet, Alya Zeinaty, Magalie Feyeux, Steven Nedellec, Julie Gavard
Summary: Glioblastoma multiforme (GBM) is a rare but devastating brain tumor in adults, with current treatments being generally ineffective. This study focused on a population of self-sustaining transformed cells with stem-like properties (GSCs) within GBM, which play a crucial role in tumor initiation, growth, invasion, and recurrence. An ex vivo model was established to explore the invasive behavior of GSCs, and it was found that the glycoprotein Neuropilin-1 (NRP1) contributes to their spreading and invasion. This study proposes a reliable approach to studying invasive properties of patient glioma cells and identifies NRP1 as a mediator in this malignant process.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Reihaneh Alsadat Mahmoudian, Maryam Lotfi Gharaie, Mohammad Reza Abbaszadegan, Ali Alasti, Mohammad Mahdi Forghanifard, Atena Mansouri, Mehran Gholamin
Summary: This study investigated the expression of MMP-13, TWIST1, and CD44 in ESCC and found significant correlations with clinical features, such as tumor progression and invasion. The results suggest that the TWIST1-CD44-MMP-13 axis may be involved in tumor aggressiveness and could serve as diagnostic markers and therapeutic targets in ESCC.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Oncology
Lei Wei, Mika Pan, Qiulan Jiang, Beiquan Hu, Jianyi Zhao, Chun Zou, Liechun Chen, Chunhai Tang, Donghua Zou
Summary: This study found that Eukaryotic Initiation Factor 4A-3 (EIF4A3) is upregulated in glioblastoma (GBM) and high expression is associated with poor prognosis. Further experiments showed that EIF4A3 promotes proliferation and migration of GBM cells and interacts with the Notch1 signaling pathway. Therefore, EIF4A3 may serve as a potential prognostic factor for GBM.
Article
Biochemistry & Molecular Biology
Matthew Thomas Ferreira, Juliano Andreoli Miyake, Renata Nascimento Gomes, Fabio Feitoza, Pollyana Bulgarelli Stevannato, Andrew Silva da Cunha, Fernanda de Oliveira Serachi, Alexandros Theodoros Panagopoulos, Alison Colquhoun
Summary: The study demonstrated the importance of PGE(2) signaling through the EP2 and EP4 receptors in controlling GBM cell biology, as well as a relationship between COX1 and MMP2 in these cells. These findings suggest a potential novel therapeutic target for drug development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Han-Soo Kim, Ha Jeong Kim, Mi Ra Lee, Ilkyu Han
Summary: The study found that the expression level of EMMPRIN in osteosarcoma is associated with metastasis-free survival, and EMMPRIN plays an important role in osteosarcoma metastasis by regulating the production of MMP and VEGF.
Article
Oncology
Jung-A Choi, Hyosun Kim, Hyunja Kwon, Elizabeth Hyeji Lee, Hanbyoul Cho, Joon-Yong Chung, Jae-Hoon Kim
Summary: This study examined the clinical value and therapeutic potential of ascitic autotaxin (ATX) in epithelial ovarian cancer (EOC). The results showed that elevated levels of ascitic ATX were associated with advanced stage, high grade, and a short disease-free period in EOC patients. Combining ascitic ATX and serum CA-125 levels improved the diagnostic accuracy for EOC, and functional studies revealed that ascitic ATX played a role in maintaining cancer stem cell-like characteristics and invadopodia formation in EOC.
BRITISH JOURNAL OF CANCER
(2023)
Article
Pharmacology & Pharmacy
Zhang'an Dai, Lin Cai, Yingyu Chen, Silu Wang, Qian Zhang, Chengde Wang, Ming Tu, Zhangzhang Zhu, Qun Li, Xianghe Lu
Summary: Brusatol can inhibit the proliferation and invasion of glioblastoma cells by suppressing the expression of ECM1, indicating its potential as a novel effective anticancer drug for glioblastoma cells.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mohammad Jalili-Nik, Amir R. Afshari, Hamed Sabri, Bahram Bibak, Hamid Mollazadeh, Amirhossein Sahebkar
Summary: The study demonstrated that Zerumbone has inhibitory effects on migration and invasion of U-87 MG GBM cells, by suppressing the enzymatic activity and protein expression of MMP-2/-9, regulating the expression levels of IL-1 beta and MCP-1 genes, as well as inhibiting the expression of Akt and p44/42 MAPK. These findings support the potential of Zerumbone as a promising phytochemical for anti-metastatic effects in the treatment of GBM.
Review
Neurosciences
Jae-Seon So, Hyeono Kim, Kyung-Seok Han
Summary: Glioblastoma is a highly malignant form of primary brain tumor with very low complete resection rate, as the GBM cells interact with various brain cells and modify the extracellular matrix to facilitate invasion. Ca2+ signaling and glutamate release play key roles in GBM cell migration and gene expression. Therapeutic interventions targeting these mechanisms may help inhibit GBM invasion.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Medicine, Research & Experimental
Preety Choudhary, Tapasi Roy, Abhishek Chatterjee, Vineet Kumar Mishra, Suyash Pant, Snehasikta Swarnakar
Summary: Forced swim stress induces gastric ulceration by promoting ROS overproduction, while melatonin provides gastroprotection by reducing inflammation burden, inhibiting MMP-3 activity, and other mechanisms.
Article
Chemistry, Analytical
Xintong Lu, Luoyu Ding, Haohan Song, Wenxin Yu, Chaoqing Dong, Jicun Ren
Summary: A novel in situ single molecule spectroscopy method based on fluorescent correlation spectroscopy (FCS) technique was proposed for measuring MMP-9 activity in single living cells. The method utilizes a fluorescent specific peptide and a reference dye as dual probes, and ratiometric translational diffusion time as the measurement parameter.
Article
Cardiac & Cardiovascular Systems
Lisa E. Dorn, William Lawrence, Jennifer M. Petrosino, Xianyao Xu, Thomas J. Hund, Bryan A. Whitson, Matthew S. Stratton, Paul M. L. Janssen, Peter J. Mohler, Anders Schlosser, Grith L. Sorensen, Federica Accornero
Summary: This study identified a novel role for nonmyocyte-derived MFAP4 in cardiac remodeling, playing a crucial role in cardiac adaptation to stress. Lack of MFAP4 resulted in increased cardiac hypertrophy, worsened cardiac function, and dysregulated G protein-coupled receptor and integrin signaling in the heart.
CIRCULATION RESEARCH
(2021)
Article
Materials Science, Multidisciplinary
Makafui Y. Folikumah, Marc Behl, Andreas Lendlein, Axel T. Neffe
Summary: In situ hydrogelation of injectable precursors upon biological stimulus can be achieved under mild conditions and has potential applications in targeted tissues or enzyme detection. This study explores the use of hydrolytic enzymes to initiate the formation of covalent hydrogel networks through a cleavage-leading-to-gelation strategy. The system consists of a 4-arm polyethylene glycol-thiodepsipeptide conjugate and a maleimide functionalized 4-armed PEG. The hydrogels formed rapidly upon addition of MMP-2 or MMP-9, demonstrating the specific input of enzymatic reactions.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2023)
Article
Developmental Biology
Tomoaki Yoshida, Kazuhide Takada, Shihoko Komine-Aizawa, Yoshimasa Kamei, Osamu Ishihara, Satoshi Hayakawa
Summary: Recent studies indicate that Lactobacillus crispatus can promote trophoblast invasion by increasing the activation of MMP-1 and MMP-2, without affecting migration. This suggests that the dominance of Lactobacillus in the endometrium may be beneficial for implantation, but further research is needed to determine if it is favorable for successful placentation at the early stage of pregnancy.
Article
Clinical Neurology
Akihiro Inoue, Takanori Ohnishi, Shohei Kohno, Shiro Ohue, Masahiro Nishikawa, Satoshi Suehiro, Shirabe Matsumoto, Saya Ozaki, Mana Fukushima, Mie Kurata, Riko Kitazawa, Seiji Shigekawa, Hideaki Watanabe, Takeharu Kunieda
Summary: GBM largely caused by GSCs that cannot be fully removed during surgery; Met-PET imaging helps identify areas with infiltrating GSCs and active tumor cells; aggressive surgical excision including Met-positive areas at TNR 1.4 is recommended for maximum resection of GBMs.
NEUROSURGICAL REVIEW
(2021)
Article
Clinical Neurology
Akihiro Inoue, Shohei Kohno, Takanori Ohnishi, Naoya Nishida, Satoshi Suehiro, Yawara Nakamura, Shirabe Matsumoto, Masahiro Nishikawa, Saya Ozaki, Seiji Shigekawa, Hideaki Watanabe, Hidenori Senba, Hironobu Nakaguchi, Mashio Taniwaki, Bunzo Matsuura, Riko Kitazawa, Takeharu Kunieda
Summary: ICG endoscopy can differentiate tumors from normal pituitary gland by evaluating elapsed times to fluorescence in each structure, aiding in achieving complete tumor resection while preserving endocrinological functions.
NEUROSURGICAL REVIEW
(2021)
Article
Biochemistry & Molecular Biology
Kazuya Miyanishi, Arisa Sato, Nanako Kihara, Ryo Utsunomiya, Junya Tanaka
Summary: Microglial cells in normal mature brains play a crucial role in phagocytosing synapses to maintain neural circuits and affect brain functions. Impaired synaptic elimination by microglia can disrupt the E/I balance, leading to various problems in memory, cognition, sleep, movement, social behaviors, and thinking. Additionally, disturbances in microglial phagocytosis functions are linked to neurological, mental, and developmental disorders like Parkinson's and Alzheimer's diseases, autism spectrum disorder, ADHD, and schizophrenia.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Oncology
Masahiro Nishikawa, Akihiro Inoue, Takanori Ohnishi, Hajime Yano, Yonehiro Kanemura, Shohei Kohno, Shiro Ohue, Saya Ozaki, Shirabe Matsumoto, Satoshi Suehiro, Yawara Nakamura, Seiji Shigekawa, Hideaki Watanabe, Riko Kitazawa, Junya Tanaka, Takeharu Kunieda
Summary: This study focused on the expression and functions of CD44 and VEGF in bevacizumab therapy. It found that GBMs expressing high P/C ratio of CD44 were more refractory to Bev and had shorter survival time. CD44 may reduce the antitumor effect of Bev and could be a useful biomarker predicting responsiveness to Bev in GBM.
Article
Clinical Neurology
Akihiro Inoue, Masahiro Nishikawa, Takanori Ohnishi, Hajime Yano, Yonehiro Kanemura, Yoshihiro Ohtsuka, Saya Ozaki, Yawara Nakamura, Shirabe Matsumoto, Satoshi Suehiro, Daisuke Yamashita, Seiji Shigekawa, Hideaki Watanabe, Riko Kitazawa, Junya Tanaka, Takeharu Kunieda
Summary: In this study, the expression of lactate dehydrogenase A (LDH-A) and pyruvate dehydrogenase (PDH) was analyzed in 20 patients with glioblastoma multiforme. The results showed that high-invasive GBM expressed LDH-A at significantly higher levels compared to low-invasive GBM, and low-invasive GBM exhibited significantly higher expression of PDH. Moreover, highly invasive GSC lines had higher LDH-A expression and lower PDH expression, along with lower oxygen consumption and adenosine triphosphate production, compared to low-invasive GSC lines.
WORLD NEUROSURGERY
(2021)
Article
Neurosciences
Satoshi Tada, Mohammed E. Choudhury, Madoka Kubo, Rina Ando, Junya Tanaka, Masahiro Nagai
Summary: This study demonstrates the potential ameliorative effects of zonisamide on microglial mitochondrial dysfunction in Parkinson's disease, providing novel evidence for its clinical use as an antiparkinsonian drug.
Article
Clinical Neurology
Akihiro Inoue, Hideaki Watanabe, Takuya Kondo, Eiji Katayama, Yukihiro Miyazaki, Satoshi Suehiro, Daisuke Yamashita, Mashio Taniwaki, Mie Kurata, Seiji Shigekawa, Riko Kitazawa, Takeharu Kunieda
Summary: This study investigates the usefulness and significance of intraoperative rapid immunohistochemistry (IHC) in the treatment of primary central nervous system lymphoma (PCNSL). The results show that intraoperative rapid IHC plays an important role in determining treatment strategies and facilitating early initiation of chemotherapy.
Review
Oncology
Saya Ozaki, Kanta Mikami, Takeharu Kunieda, Junya Tanaka
Summary: CLICs are a type of ion channel proteins with potential as prognostic markers in cancer. This review focuses on CLIC2 and discusses its role in the metastasis and invasion of malignant tumor cells. CLIC2 may prevent malignant cell invasion and metastasis by inhibiting the activity of matrix metalloproteinase 14.
Article
Neuroimaging
Akihiro Inoue, Hideaki Watanabe, Satoshi Suehiro, Naoya Nishida, Yasuhiro Shiraishi, Taichi Furumochi, Yoshihiro Takimoto, Takanori Ohnishi, Seiji Shigekawa, Takeharu Kunieda
Summary: The clinical usefulness of zero-echo-time (ZTE)-based MRI in planning surgical approach and providing anatomical guidance during transcranial surgery was evaluated. Results showed that ZTE/MRA fusion images and 3D ZTE-based MRI models allowed clear visualization of cranial and intracranial morphology without radiation exposure or the use of contrast medium. However, ZTE-based MRI was not helpful in surgery for skull base lesions. This low-invasive technology was useful for preoperative planning and guidance in a subset of neurosurgical diseases.
NEURORADIOLOGY JOURNAL
(2023)
Article
Clinical Neurology
Akihiro Inoue, Shirabe Matsumoto, Takanori Ohnishi, Yukihiro Miyazaki, Shingo Kinnami, Kazuhisa Kanno, Takatsugu Honda, Mie Kurata, Mashio Taniwaki, Kosuke Kusakabe, Satoshi Suehiro, Daisuke Yamashita, Seiji Shigekawa, Hideaki Watanabe, Riko Kitazawa, Takeharu Kunieda
Summary: PCNSL and GBM can be accurately differentiated through various examinations, including measurements of serum soluble interleukin-2 receptor and 02-microglobulin in cerebrospinal fluid, as well as imaging studies and tumor markers. The concentrations of 02-microglobulin in CSF ≥2.0 mg/L and tumor-to-contralateral normal brain tissue ratio ≥2.4 on 18F-FDG-PET can quantitatively differentiate PCNSL from GBM, which may serve as clinically useful indicators.
WORLD NEUROSURGERY
(2023)
Article
Cell Biology
Ryo Utsunomiya, Kanta Mikami, Tomomi Doi, Mohammed E. Choudhury, Toshihiro Jogamoto, Naohito Tokunaga, Eiichi Ishii, Mariko Eguchi, Hajime Yano, Junya Tanaka
Summary: In addition to genetic factors, environmental factors also play a significant role in the development of attention deficit/hyperactivity disorder (ADHD). This study used Lister hooded rats as ADHD model animals and found that environmental conditions had a notable impact on the behavior and neural activity of the rats. Specifically, rats raised in an enriched environment showed improved ADHD-like behaviors, which were associated with reduced neuronal activity in the medial prefrontal cortex.
Article
Neurosciences
Mohammed E. Choudhury, Saya Ozaki, Noriyuki Miyaue, Taisei Matsuura, Kanta Mikami, Afsana Islam, Madoka Kubo, Rina Ando, Hajime Yano, Takeharu Kunieda, Masahiro Nagai, Junya Tanaka
Summary: Activated microglia potentially cause neurodegeneration in Parkinson's disease (PD). CLIC2 increases MMP-9 activity in microglia, which is involved in the pathogenesis of PD.
Article
Rehabilitation
Satoru Taguchi, Mohammed E. Choudhury, Kanta Mikami, Ryo Utsunomiya, Hajime Yano, Junya Tanaka
Summary: This study suggests that long-term exercise represses early response genes, increases resistance to stress, diminishes anxiety-related behavior, and improves social behavior.
ANNALS OF REHABILITATION MEDICINE-ARM
(2022)
Review
Oncology
Akihiro Inoue, Takanori Ohnishi, Masahiro Nishikawa, Yoshihiro Ohtsuka, Kosuke Kusakabe, Hajime Yano, Junya Tanaka, Takeharu Kunieda
Summary: Recurrence in glioblastoma may be caused by surviving glioma stem-like cells expressing high levels of CD44, which promote invasion and proliferation of tumor cells via various signaling pathways. Severe hypoxia activates genes related to cell invasion, whereas moderate hypoxia activates genes related to cell proliferation.
Article
Biochemistry & Molecular Biology
Akihiro Inoue, Takanori Ohnishi, Masahiro Nishikawa, Hideaki Watanabe, Kosuke Kusakabe, Mashio Taniwaki, Hajime Yano, Yoshihiro Ohtsuka, Shirabe Matsumoto, Satoshi Suehiro, Daisuke Yamashita, Seiji Shigekawa, Hisaaki Takahashi, Riko Kitazawa, Junya Tanaka, Takeharu Kunieda
Summary: This study analyzed the relationship between CD44 expression and magnetic resonance imaging (MRI)-based invasiveness of recurrent glioblastoma multiforme (GBM). High-invasive GBM showed significantly higher CD44 expression and was associated with worse survival. In addition, the study investigated the relationship between 5-aminolevulinic acid (5-ALA) spectroscopy-based tumor invasiveness and CD44 expression.
