Article
Zoology
Qiang Yang, Chuan-Min Qiao, Wei-Wei Liu, Hao-Yun Jiang, Qi-Qi Jing, Ya-Ya Liao, Jun Ren, Yu-Yun Xing
Summary: Through CRISPR/Cas9 editing, we unexpectedly obtained BMPR-IB knockout and mutant piglets while attempting to generate g.A746G substitution in the BMPR-IB gene. These piglets exhibited severe skeletal dysplasia and showed differential protein expression compared to the wild-type controls.
ZOOLOGICAL RESEARCH
(2022)
Article
Dentistry, Oral Surgery & Medicine
Danyang Song, Sui Yang, Tao Tan, Ruijie Wang, Zeyun Ma, Yixiang Wang, Lei Wang
Summary: This study investigated the role of ODAM in promoting junctional epithelium-related gene expression in ALC through the ODAM-WNT1 axis, shedding new light on the function of ODAM and JE formation.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Environmental Sciences
Natsumi Fujiwara, Shohei Yamashita, Motoki Okamoto, Marion A. Cooley, Kazumi Ozaki, Eric T. Everett, Maiko Suzuki
Summary: In this study, it was found that perfluorooctanoic acid (PFOA) negatively affects tooth enamel development through cell death mechanisms involving necrosis/necroptosis and apoptosis, mediated by the ROS-MAPK/ERK signaling pathway. PFOA also suppressed cell proliferation and viability. This research provides initial evidence that PFOA could potentially contribute to cryptogenic enamel malformation.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Furong Xie, Xueqin Zhu, Xiao Liu, Hui Chen, Jun Wang
Summary: This study reveals the functional role of WTAP-mediated m6A RNA methylation in mammalian amelogenesis. The results show that WTAP is widely expressed in the early stages of tooth development, and its loss leads to severe developmental defects in enamel formation. Mechanistically, WTAP loss inhibits the expression of Sonic hedgehog. These findings provide new insights into the importance of m6A methylation in enamel development.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Rozana Shemirani, Gan Lin, Dawud Abduweli Uyghurturk, Michael Le, Yukiko Nakano
Summary: This study identifies direct targets of miR-exon4 and reveals the regulatory mechanism of miR-exon4 in the expression of Runx2. The findings suggest that miR-exon4 directly targets Nfia and Prkch to negatively regulate Runx2 in osteoblasts and ameloblasts.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Ji-Hye Jang, Min-Seong Kim, Ainsley Mike Antao, Won-Jun Jo, Hyung-Joon Kim, Su-Jin Kim, Myeong-Jun Choi, Suresh Ramakrishna, Kye-Seong Kim
Summary: This study identified O-cyclic phytosphingosine-1-phosphate (cP1P) as a novel factor that significantly enhances the differentiation potential of human embryonic stem cells (hESCs) into cardiomyocytes. Treatment with cP1P was found to increase the beating colony number and contracting area of cardiomyocytes. Furthermore, the molecular mechanism of cP1P regulating SMAD1/5/8 signaling via the ALK3/BMP receptor cascade during cardiac differentiation was elucidated. The results provide a new insight for using cP1P to improve the quality of cardiomyocyte differentiation for regenerative therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Fen Xiao, Chen-Yi Tang, Hao-Neng Tang, Hui-Xuan Wu, Nan Hu, Long Li, Hou-De Zhou
Summary: This study revealed that Lnc-FR332443 inhibits adipogenesis by regulating the expression of Runx1, suggesting it as a potential drug target for obesity prevention and treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Akiko Suzuki, Hiroki Yoshioka, Teng Liu, Aania Gull, Naina Singh, Thanh Le, Zhongming Zhao, Junichi Iwata
Summary: This study reveals the regulatory network of genes and microRNAs (miRNAs) related to amelogenesis imperfecta. miR-16-5p and miR-27b-3p were identified as potential pathogenic miRNAs, as they significantly inhibited ameloblast differentiation by regulating mAIGenes.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Xinchao Miao, Kunimichi Niibe, Maolin Zhang, Zeni Liu, Praphawi Nattasit, Yumi Ohori-Morita, Takashi Nakamura, Xinquan Jiang, Hiroshi Egusa
Summary: Amelogenin is a major component of enamel proteins, but the role of Amelx transcriptional activation in regulating ameloblast differentiation from iPSCs is unclear. This study induced Amelx expression in mouse iPSCs and used a three-stage strategy to differentiate cells into ameloblast lineage. Lithium chloride was found to enhance differentiation of dental epithelial cells, leading to the formation of ameloblast lineage with specific markers and calcium deposition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Kokoro Iwata, Keita Kawarabayashi, Keigo Yoshizaki, Tian Tian, Kan Saito, Asuna Sugimoto, Rika Kurogoushi, Aya Yamada, Akihito Yamamoto, Yasuei Kudo, Naozumi Ishimaru, Satoshi Fukumoto, Tsutomu Iwamoto
Summary: The study demonstrated the role of a newly identified ECM protein, Vwde, in regulating cell behaviors and enamel formation in teeth. Knockout mice lacking Vwde showed enamel hypoplasia phenotype.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xinchao Miao, Kunimichi Niibe, Yunyu Fu, Maolin Zhang, Praphawi Nattasit, Yumi Ohori-Morita, Takashi Nakamura, Xinquan Jiang, Hiroshi Egusa
Summary: This study reveals that Epfn activation promotes ameloblast induction from mouse iPSCs by upregulating BMP-Smad activity, suggesting Epfn as a promising target for enamel and tooth regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuyeon Jang, Tae Sub Park, Byung-Chul Park, Young Mok Lee, Tae-Hwe Heo, Hyun Sik Jun
Summary: This study investigated the impact of G6PT deficiency on monocyte-to-macrophage differentiation and found that G6PT-deficient monocytes exhibit immature differentiation into macrophages. Additionally, G6PT deficiency is associated with abnormal glucose metabolism and reduced secretion of inflammatory cytokines. These findings highlight the significance of G6PT in maintaining glucose homeostasis and supporting immune response in GSD-Ib.
Article
Biochemistry & Molecular Biology
Wenjie Yu, Cheng-Zhen Chen, Yanxia Peng, Ze Li, Yan Gao, Shuang Liang, Bao Yuan, Nam-Hyung Kim, Hao Jiang, Jia-Bao Zhang
Summary: In this study, it was found that inhibition of KRAS in 3T3-L1 and C2C12 cells altered the expression of key proteins involved in cell proliferation, autophagy, and adipogenic differentiation, leading to reduced proliferation capacity, enhanced differentiation into mature adipocytes, and increased lipid accumulation. Additionally, KRAS inhibition decreased the activation levels of several signaling pathways during differentiation. These results demonstrate the unique regulatory effects of KRAS on cell processes related to adipogenesis and lipid accumulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Caihong Fang, Mingli He, Di Li, Qiong Xu
Summary: Aberrant elevation of osteoclast differentiation and function in inflammatory bone diseases is regulated by YTHDF2, which plays a negative regulatory role via the NF-kappa B and MAPK signaling pathways.
CELLULAR SIGNALLING
(2021)
Article
Biochemistry & Molecular Biology
Dong-Feng Yuan, Hao-Ran Wang, Zong-Fei Wang, Guang-Hui Liang, Wen-Qun Xing, Jian-Jun Qin
Summary: The study revealed that circZMYM4 overexpression suppressed proliferation, metastasis, and apoptosis of LUAD cells, while miR-587 overexpression weakened these effects. CircZMYM4 regulates the miR-587/ODAM axis to suppress LUAD progression.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Kyeong-Rok Kang, Jae-Sung Kim, Jeong-Yeon Seo, Hyang Lim, Tae-Hyeon Kim, Sun-Kyoung Yu, Heung-Joong Kim, Chun Sung Kim, Hong Sung Chun, Joo-Cheol Park, Do Kyung Kim
Summary: The present study investigates the role of NAMPT in odontogenic differentiation during tooth development in mice. The up-regulation of NAMPT promotes odontogenic differentiation and mineralization, while the inhibition of NAMPT attenuates odontogenic differentiation.
KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Hoon Seonwoo, Han-Wool Choung, Sangbae Park, Kyoung Soon Choi, Kyoung-Je Jang, Jangho Kim, Ki-Taek Lim, Yeonju Kim, Pankaj Garg, Shambhavi Pandey, Juo Lee, Joo-Cheol Park, Yun-Hoon Choung, Pill-Hoon Choung, Soo Young Kim, Jong Hoon Chung
Summary: Natural calcium phosphate cements (CPCs) derived from sintered animal bone have low mechanical strength, but incorporating reduced graphene oxide (RGO) and applying pulsed electromagnetic fields (PEMFs) can significantly improve their mechanical properties and osteogenic differentiation ability. The chemical bonding between RGO and CPCs enhances the mechanical properties, while PEMFs enhance osteogenic differentiation of stem cells. RGO-CPCs with PEMFs show improved biocompatibility and osteoinductivity in both in vitro and in vivo studies.
Article
Dentistry, Oral Surgery & Medicine
Shengfeng Bai, Ji-Hyun Lee, Chul Son, Dong-Seol Lee, Joo-Cheol Park
Summary: The study demonstrates that CPNE7 plays a regulatory role in periodontal ligament (PDL) repair by supporting PDL alignment through TAU-mediated cytoskeleton reorganization and direct regulation of CAP-mediated PDL attachments of PDLCs.
JOURNAL OF CLINICAL PERIODONTOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yoon Seon Lee, Yeoung-Hyun Park, You-Mi Seo, Hye-Kyung Lee, Joo-Cheol Park
Summary: This study identified that the Smad4-dependent TGF-beta 1/BMP2 signaling pathway regulates the expression of Cpne7 in dental epithelial cells, and the CPNE7 protein mediates odontoblast differentiation through epithelial-mesenchymal interaction. The results showed that conditioned medium treated with TGF-beta 1/BMP2 can induce the formation of tubular dentin.
Article
Cell Biology
Hye Kyung Lee, Ludwig Knabl, Juan Moliva, Ludwig Knabl, Anne P. Werner, Seyhan Boyoglu-Barnum, Sebastian Kapferer, Birgit Pateter, Mary Walter, Nancy J. Sullivan, Priscilla A. Furth, Lothar Hennighausen
Summary: The study reveals that octogenarians who were previously infected with SARS-CoV-2 demonstrate a sustained IgG antibody response for 15 months and exhibit a 35-fold increase in antibody levels after receiving a single dose of the BNT162b2 mRNA vaccine. In contrast, antibody responses in naive individuals only increase by 6-fold after the second vaccine dose. Furthermore, the previously infected elderly individuals show higher spike-specific ACE2 antibody binding responses after two vaccine doses compared to the naive cohort. RNA sequencing analysis indicates the activation of interferon-induced genetic programs in the previously infected individuals, and a preferential increase of specific IGHV clonal transcripts is observed only in the previously infected nuns.
Article
Multidisciplinary Sciences
Hye Kyung Lee, Jinyoung Go, Heungsup Sung, Seong Who Kim, Mary Walter, Ludwig Knabl, Priscilla A. Furth, Lothar Hennighausen, Jin Won Huh
Summary: Heterologous ChAdOx1-BNT162b2 vaccination induces a stronger immune response than BNT162b2-BNT162b2 vaccination, as evidenced by increased antibody levels against the ancestral SARS-CoV-2 strain and activation of interferon pathways. This study also revealed enriched B cell and CD4(+) T cell responses, as well as a stronger clonal expansion of memory B cells with ChAdOx1-BNT162b2 vaccination.
Article
Immunology
Hye Kyung Lee, Ludwig Knabl, Mary Walter, Ludwig Knabl, Yuhai Dai, Magdalena Fuessl, Yasemin Caf, Claudia Jeller, Philipp Knabl, Martina Obermoser, Christof Baurecht, Norbert Kaiser, August Zabernigg, Gernot M. Wurdinger, Priscilla A. Furth, Lothar Hennighausen
Summary: Antibody response following Omicron infection is less robust, but prior vaccination can enhance the response. Vaccinated patients show significant antibody levels and neutralizing activity immediately after infection, while unvaccinated patients do not. Antibody levels increase within two weeks, with vaccinated individuals having higher neutralizing activity compared to those with prior infection. The transcriptomic response in vaccinated patients shows a blunted interferon response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hye Ri Gug, Yeoung-Hyun Park, Su-Jin Park, Ji Yeon Jang, Ji-Hyun Lee, Dong-Seol Lee, Won -Jun Shon, Joo-Cheol Park
Summary: This study evaluated the potential application of CPNE7-derived functional peptide (CPNE7-DP) in dentin caries. The results showed that CPNE7-DP was stable in caries-like environments and regenerated tubular tertiary dentin, alleviating inflammation and reducing the expression of inflammatory cytokines. CPNE7-DP may help overcome the limitations of current restorative caries treatments.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Dong-Seol Lee, Yeo Joon Song, Hye Ri Gug, Ji-Hyun Lee, Hyun Sook Bae, Joo-Cheol Park
Summary: Tooth development involves cell divisions and interactions, and the NFI-C transcription factor plays a crucial role. This study reveals that Nfic regulates cell proliferation in various mineralized tissue stem cells, and is involved in the expression of pluripotency genes. Especially in dental epithelial stem cells, Nfic regulates epithelial cell proliferation through epithelial-mesenchymal interactions.
STEM CELLS INTERNATIONAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hyang Lim, Ji-Su Oh, Kyeong-Rok Kang, Jeong-Yeon Seo, Do Kyung Kim, Sun-Kyoung Yu, Heung-Joong Kim, Joo-Cheol Park, Jae-Sung Kim
Summary: This study found that 25-HC can induce odontoclastogenesis in MDPC-23 cells by modulating the RANK-RANKL-OPG axis, potentially contributing to the pathophysiological processes of internal tooth resorption.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Juhyun Lee, Geumbit Hwang, Hyeri Gug, Ji-Hyun Lee, Su-Jin Park, Joo-Cheol Park
Summary: This study investigated the effects of toothpastes containing CPNE7-derived oligopeptide (CPNE7-DP) and other active desensitizing ingredients on dentin microleakage, tubule occlusion, and tertiary dentin formation. The results showed that toothpaste with CPNE7-DP had a long-term dentin sealing effect and promoted the formation of new hard tissue.
Article
Hematology
Hye Kyung Lee, Manuela A. Hoechstetter, Maike Buchner, Trang Thu Pham, Jin Won Huh, Katharina Mueller, Sabine Zange, Heiner von Buttlar, Philipp Girl, Roman Woelfel, Lisa Brandmeier, Lisa Pfeuffer, Priscilla A. Furth, Clemens -Martin Wendtner, Lothar Hennighausen
Summary: Patients with CLL treated with B-cell pathway inhibitors and anti-CD20 antibodies have low humoral response rates after SARS-CoV-2 vaccination. A study compared the transcriptional response of peripheral blood mononuclear cells with antibody and T-cell response rates in CLL/SLL patients who received heterologous BNT162b2/ChAdOx1 vaccination. The antibody response rate after two doses was 40%, which increased to 53% after the booster dose. The T-cell response rate after two doses was 80%, increasing to 93% after the booster.
Article
Biology
Markus Hoffmann, Nico Trummer, Leon Schwartz, Jakub Jankowski, Hye Kyung Lee, Lina-Liv Willruth, Olga Lazareva, Kevin Yuan, Nina Baumgarten, Florian Schmidt, Jan Baumbach, Marcel H. Schulz, David B. Blumenthal, Lothar Hennighausen, Markus List
Summary: The study developed an automated pipeline that prioritizes tissue-specific transcription factors (TFs) from multimodal data and generates an interactive web report. The pipeline successfully identified known TFs as well as previously unreported TFs active in lactating mouse mammary glands. Additionally, the researchers studied a variety of ENCODE datasets and observed assay-specific differences.
Article
Multidisciplinary Sciences
Geumbit Hwang, Hyejin Seo, Joo-Cheol Park
Summary: Cpne7 deficiency leads to hepatic lipid metabolism abnormalities, excessive ROS production, and mitochondrial dysfunction. These findings are important for understanding the pathogenesis of NAFLD and suggest Cpne7 as a potential therapeutic target for NAFLD.
