Article
Multidisciplinary Sciences
Eugenia Wulff-Fuentes, Jeffrey Boakye, Kaeley Kroenke, Rex R. Berendt, Carla Martinez-Morant, Michaela Pereckas, John A. Hanover, Stephanie Olivier-Van Stichelen
Summary: The study investigates the role of O-GlcNAcylation in regulating the solubility and aggregation of OTX2 protein, potentially preventing OTX2 aggregation through its interaction with CCT5 protein. Additionally, O-GlcNAc-depleted OTX2 is detrimental to cancer cells.
Article
Biochemistry & Molecular Biology
Feng Liu, Yiling Cao, Chun Zhang, Hua Su
Summary: Glomerulosclerosis is a major histopathological change in diabetic kidney diseases, characterized by excessive deposition of extracellular matrix in the glomerulus. TGF-beta has been implicated as a major pathogenic factor, but current monoclonal antibody therapies have been ineffective. This study identified a novel lncRNA, DANCR, which acts as a negative regulator of TGF-beta/Smad signaling in mesangial cells. DANCR stabilizes NLK mRNA, leading to enhanced phosphorylation of activated Smad2/3 in the nucleus. This discovery provides a potential therapeutic target against diabetic glomerulosclerosis.
Article
Medicine, Research & Experimental
Ling Hu, Xi Pan, Jinyue Hu, Hong Zeng, Xueting Liu, Manli Jiang, Binyuan Jiang
Summary: Proteasome inhibitors can reduce the lethal effect of paclitaxel on nasopharyngeal cancer cells, and their combination with paclitaxel interferes with the release of apoptotic signals such as MCL1, BCL2, and Caspase-9.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Jackie Trink, Usman Ahmed, Kian O'Neil, Renzhong Li, Bo Gao, Joan C. C. Krepinsky
Summary: Diabetic kidney disease (DKD) is characterized by glomerular accumulation of extracellular matrix (ECM) proteins, and high glucose (HG) induction of glomerular mesangial cell (MC) profibrotic responses plays a central role in its pathogenesis. The endoplasmic reticulum resident GRP78 translocates to the cell surface in response to HG and mediates Akt activation and downstream profibrotic responses in MC. This study investigates whether cell surface GRP78 (csGRP78) regulates thrombospondin 1 (TSP1) expression and TGF beta 1 activation in response to HG.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Food Science & Technology
Yingbo Peng, Wanhong He, Shuang Teng, Muneer Ahmed Jamali, Severiano R. Silva, Alfredo Jorge Costa Teixeira
Summary: In this study, we investigated the degradation of intramuscular connective tissue (IMCT) using cathepsin L. Our findings showed that cathepsin L mainly degrades the non-helical peptides of collagen in IMCT, rather than the triple helix region. We also observed that cathepsin L has a more pronounced degradation effect on the structural protein DCN at a pH of 5.0. Additionally, cathepsin L treatment resulted in a more significant decrease in the thermal denaturation temperatures (T-P and T-O) of IMCT, especially at a pH of 5.0.
Article
Medicine, General & Internal
Zhonghui Zhang, Liang Zhong, Wenran Dan, Xuan Chu, Chen Liu, Xu Luo, Peng Wan, Zhenyan Liu, Yang Lu, Xiao Wang, Beizhong Liu
Summary: The research reveals that ZFP91 enhances AML cell proliferation and inhibits apoptosis by interacting with RIP1 and inhibiting its K48-linked ubiquitination. These findings provide evidence that targeted inhibition of ZFP91 could be a potential approach to treat AML.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Michael Gekle, Sigrid Mildenberger
Summary: Mineralocorticoid aldosterone induces cytosolic alkalization in rat glomerular mesangial cells through NHE and ERK1/2 activation, promoting cell proliferation and collagen secretion.
Article
Chemistry, Physical
Tianhao Wang, Jianbo Tong, Xing Zhang, Hao Luo, Lei Xu, Zhe Wang
Summary: Protein degradation and synthesis are crucial for regulating biological activities. USP14, a member of DUBs, plays a critical role in protein degradation. Abnormal expression of USP14 is associated with malignant tumors and other diseases.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Jeffrey C. Hsiao, Atara R. Neugroschl, Ashley J. Chui, Cornelius Y. Taabazuing, Andrew R. Griswold, Qinghui Wang, Hsin-Che Huang, Elizabeth L. Orth-He, Daniel P. Ball, Giorgos Hiotis, Daniel A. Bachovchin
Summary: CARD8, a pattern-recognition receptor, is involved in the activation of the inflammasome. The degradation of CARD8 by the 20S proteasome plays a crucial role in the control of inflammasome activation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Engineering, Biomedical
Ruiqi Wang, Boyang Chen, Haiying Wei, Wei Yan, Yuping Wu, Cao Wang, Bosong Zhang, Fengzhen Liu, Hui Tian, Xiongbiao Chen, Weiming Tian
Summary: This study investigates the use of O-HA + D hydrogels to deactivate TGF-beta 1 and reduce scar formation in glaucoma filtration surgery. The results demonstrate that O-HA + D hydrogels can effectively lower the concentration of TGF-beta 1 in aqueous humor, inhibit collagen production, and reduce inflammation and collagen degradation in wounds.
MATERIALS TODAY BIO
(2022)
Article
Food Science & Technology
Yaolei Zhang, Mengmeng Li, Yuanxiao Liu, Erqi Guan, Ke Bian
Summary: This study investigated the degradation of AFB(1) by water-assisted microwave irradiation, showing that WMI effectively degrades AFB(1) following pseudo-first-order reaction kinetics. Six new main degradation products were identified, and a possible degradation pathway was proposed using ultra-high-performance liquid chromatography Q-Orbitrap mass spectrometry. The variety and quantity of AFB(1) degradation products and the degradation pathway were influenced by microwave heating conditions.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Yanhua Xu, Huiyan Dong, Chenxi Liu, Haiwei Lou, Renyong Zhao
Summary: In this study, a bacterium called Pseudomonas aeruginosa M-4 was isolated from rotten wood, and its culture supernatant showed a high degradation rate of 56.79% for the mycotoxin Aflatoxin B1 (AFB1). Treatment with certain substances decreased the AFB1 degradation rate, but heating the culture supernatant increased it significantly. The bacterium also reduced the content of AFB1 in contaminated maize and transformed it into structurally different compounds with potentially lower toxicity.
Article
Agriculture, Dairy & Animal Science
Monika Jamiol, Jacek Wawrzykowski, Kamila Bulak, Marta Kankofer
Summary: The protein N-glycosylation and O-glycosylation have a modulating effect on the adhesion and viability of placental cells during early-mid pregnancy. Decorin and tunicamycin were shown to have anti-adhesive properties with respect to caruncular cells of the pregnant bovine uterus.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Review
Chemistry, Medicinal
Marianne E. Maresh, Andres F. Salazar-Chaparro, Darci J. Trader
Summary: Regulating protein production and degradation is crucial for cellular homeostasis, with the proteasome playing a key role in maintaining proper protein levels. Disease states such as cancer and neurodegenerative diseases can impact proteasome activity, making it a potential target for therapeutic intervention. Studies involving chemical modulators and inhibitors have shed light on the proteasome's therapeutic potential.
FUTURE MEDICINAL CHEMISTRY
(2021)
Article
Food Science & Technology
Wei Li, Wenjun Li, Chao Zhang, Ning Xu, Caixia Fu, Chao Wang, Deyuan Li, Qian Wu
Summary: The degradation conditions of aflatoxin B1 (AFB1) by T. halophilus in MRS liquid culture were investigated. Factors such as the inoculum amount, fermentation time, and fermentation temperature were examined, and the degradation rate of AFB1 was measured. The study found that AFB1 degradation increased with temperature, reaching a maximum value of 75.9% +/- 4.92%. However, at temperatures exceeding 32 degrees C, the degradation rate decreased. Furthermore, the activity of AFB1 degradation by T. halophilus was influenced by the starting concentration of the strain and the presence of certain metal ions.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
