Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 109, Issue 4, Pages 747-754Publisher
WILEY-BLACKWELL
DOI: 10.1002/jcb.22453
Keywords
HUMAN UMBILICAL CORD; MESENCHYMAL STEM CELL; GERM LIKE CELLS; IN VITRO DIFFERENTIATION
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Funding
- National Natural Science Foundation of China [30772354]
- Science and Technology Planning Project of Guangdong Province, China [2006B35901013, 20078080701022]
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Recent studies have demonstrated that mesenchymal stem cells could differentiate into germ cells under appropriate conditions. We sought to determine whether human umbilical cord Wharton's jelly-derived mesenchymal stem cells (HUMSCs) could form germ cells in vitro. HUMSCs were induced to differentiate into germ cells in all-trans retinoic acid, testosterone and testicular-cell-conditioned medium prepared from newborn male mouse testes. HUMSCs formed tadpole-like cells after induction with different reagents and showed both mRNA and protein expression of germ-cell-specific markers Oct4 (POUE5), Ckit, CD49(f) (alpha 6), Stella (DDPA3), and Vasa (DDX4). Our results may provide a new route for reproductive therapy involving HUMSCs and a novel in vitro model to investigate the molecular mechanisms that regulate the development of the mammalian germ lineage. J. Cell. Biochem. 109: 747-754, 2010. (C) 2010 Wiley-Liss. Inc.
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