Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 106, Issue 4, Pages 666-672Publisher
WILEY
DOI: 10.1002/jcb.22053
Keywords
NM23/NDPK; NUCLEOSIDE DIPHOSPHATE KINASE; MELANOMA M14 CELLS; IFI16; P53; cMYC; DNA-protein cross-linkage; ChIP
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Funding
- Universita di Roma to the Faculty of Pharmacy
- Faculty of Biological Sciences (MES)
- Fondazione Enrico ed Enrica Sovena
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In the melanoma M14 cell line, we found that the antimetastatic protein NM23/nucleoside diphosphate kinase binds to the promoters Of the oncogene cMYC and of P53, a gene often mutated in human cancer(Cervoni et al. [2006] J. Cell. Biochem. 98:421-428). In a further study, we find now that IFI16, a transcriptional repressor, in both promoters binds to the G-rich fragment that also binds NM23/NDPK. These fragments possess non-B DNA structures. Moreover, by sequential chromatin immunoprecipitation (re-ChIP) we show that the two proteins (IFI16 and NM23/NDPK) are simultaneously bound in vivo to the same DNA fragments. Since P53 stimulates apoptosis and inhibits cellular growth, and cMYC promotes cell growth and, in several instances, also apoptosis, the presence of NM23 and IFI16 on the same DNA fragments suggests their common involvement in the reduced development of some tumors. J. Cell. Biochem. 106: 666-672, 2009. (C) 2009 Wiley-Liss, Inc.
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