Review
Cell Biology
Candice Qiu Xia Yam, Hong Hwa Lim, Uttam Surana
Summary: The article mainly discusses the role of the DNA damage checkpoint in cell cycle regulation, and how cells repair and continue cell cycle progression when damaged. It also mentions a potential unusual cellular response that cells may take when corrective measures fail.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Osama Garwain, Xiaoming Sun, Divya Ramalingam Iyer, Rui Li, Lihua Julie Zhu, Paul D. Kaufman
Summary: The depletion of Ki-67 in vertebrate mammals can cause DNA damage, particularly in mitotic cells. The C-terminal chromatin-binding domain of Ki-67 plays a crucial role in protecting cells from this damage. Simultaneous depletion of Ki-67 and p53 results in increased chromosome bridges and micronuclei, highlighting the importance of Ki-67 in maintaining genome stability.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Cell Biology
Fernando Luna-Maldonado, Marco A. Andonegui-Elguera, Jose Diaz-Chavez, Luis A. Herrera
Summary: This review highlights the importance of genomic stability for cellular function, and discusses the crosstalk between SAC and DDR proteins throughout the cell cycle, providing new insights into maintaining genome stability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shuangyan Yao, Yuting Feng, Yan Zhang, Jinrong Feng
Summary: Cells face constant genetic assaults that cause DNA damage, leading to genome instability. Eukaryotic cells have evolved a DNA damage response system to monitor and repair these lesions. Studies show that DDR genes in pathogenic fungus Candida albicans are functionally similar to those in Saccharomyces cerevisiae, but may act through distinct mechanisms, particularly in resisting DNA damage stress induced by reactive oxygen species.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Cell Biology
Marina Rodriguez-Munoz, Martina Serrat, David Soler, Anna Genesca, Teresa Anglada
Summary: Chromosome bridges in cancer cells are often formed and may break during mitosis, with a direct correlation between the distance between bridge kinetochores and bridge breakage. The mechanisms responsible for chromosome bridge breakage during mitosis may depend on the separation between the bridge kinetochores. Previous studies have suggested mechanical stress or biochemical digestion as possible causes of bridge breakage in interphase cells, suggesting a multifactorial model for bridge breakage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Iraia Garcia-Santisteban, Alba Llopis, Lenno Krenning, Jon Vallejo-Rodriguez, Bram van den Broek, Ana M. Zubiaga, Rene H. Medema
Summary: ATM becomes dispensable for G1 checkpoint maintenance as early as 1 hour after DSB induction, while CHK2 kinase activity is necessary to maintain G1 arrest independently of other proteins, suggesting that the G1 arrest is sustained in a lesion-independent manner.
Review
Oncology
Ignacio Campillo-Marcos, Raul Garcia-Gonzalez, Elena Navarro-Carrasco, Pedro A. Lazo
Summary: VRK1 is a nuclear Ser-Thr chromatin kinase that is not mutated in cancer, but is overexpressed in many types of tumors and linked to poor prognosis. It plays crucial roles in regulating cell proliferation and DNA damage response pathways, having both pro-tumorigenic effects and protective anti-tumor roles depending on the cellular context.
Article
Biochemistry & Molecular Biology
Thomas Eekhout, Jose Antonio Pedroza-Garcia, Pooneh Kalhorzadeh, Geert De Jaeger, Lieven De Veylder
Summary: During DNA replication, the WEE1 kinase inhibits cyclin-dependent kinases (CDKs) to safeguard genomic integrity. Wee1 mutant plants are hypersensitive to replication stress due to their inability to respond properly to DNA replication problems. The pol alpha-2 mutant, with a mutated catalytic subunit of DNA polymerase alpha, acts as a suppressor mutant of wee1 by causing instability and interaction loss, leading to a prolonged S-phase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Scott Bachus, Drayson Graves, Lauren Fulham, Nikolas Akkerman, Caelan Stephanson, Jessica Shieh, Peter Pelka
Summary: The NIMA family of serine/threonine kinases is involved in diverse cellular processes, and its members have been studied in various organisms. The roles of NIMA kinases in mitosis and cell division have been well-established, but their functions beyond these processes remain poorly understood. The mammalian Nek family of kinases, in particular, requires further investigation to uncover their diverse roles in cellular activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Congqi Shi, Kaiyu Qin, Anqi Lin, Aimin Jiang, Quan Cheng, Zaoqu Liu, Jian Zhang, Peng Luo
Summary: This study summarizes currently identified and promising biomarkers for predicting the response of oncology patients to immune checkpoint inhibitors, and explores the mechanism of combination therapy with immune checkpoint inhibitors and DNA damage repair inhibitors.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Cell Biology
Inmaculada Ayala, Antonino Colanzi
Summary: The Golgi complex plays a central role in secretory traffic and can form a structure called the Golgi ribbon. The balanced formation and cleavage of membrane tubules connecting the stacks are crucial for cell division and spindle formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xianghua Zhang, Ji Eun Park, Eun Ho Kim, Jihee Hong, Ki-Tae Hwang, Young A. Kim, Chang-Young Jang
Summary: The timely and temporal phosphorylation of proteins during mitosis is critical, with mitotic kinases activating and phosphatases repressing the process. The mitotic exit, or transition from mitosis to interphase, involves removal of mitotic phosphorylation by protein phosphatases, including PP1 and PP2A. A new mitotic phosphatase relay, involving Wip1/PPM1D phosphatase activity, is essential for CPC translocation in anaphase, demonstrating spatiotemporal regulation of mitotic exit to prevent tumor initiation and progression.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Styliani Iliaki, Rudi Beyaert, Inna S. Afonina
Summary: PLK1 is a Ser/Thr kinase that plays crucial roles in cell cycle regulation, DNA damage response, apoptosis, and cancer progression. Overexpression of PLK1 is associated with poor prognosis in cancer, making it an attractive therapeutic target.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Cell Biology
Patrick Partscht, Borhan Uddin, Elmar Schiebel
Summary: This study reveals that there is redundancy among the three human CDC14 paralogues, CDC14A, CDC14B, and CDC14C, and their specific roles in cell cycle control are not clear. The functions of CDC14 proteins in the cell cycle are not conserved between yeast and human cells.
JOURNAL OF CELL SCIENCE
(2021)
Article
Oncology
Radhika Koranne, Kayla Brown, Hannah Vandenbroek, William R. R. Taylor
Summary: C9ORF78 is a poorly characterized protein found in diverse eukaryotes. It has been suggested to be involved in growth regulatory pathways in malignant tissues. This study discovers a potential function of regulating the spliceosome, new subcellular localization in nuclei and association with kinetochores/centromeres in mitotic cells.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Xiao Qi Wang, Chung Mau Lo, Lin Chen, Elly S-W Ngan, Aimin Xu, Randy Y. C. Poon
CELL DEATH AND DIFFERENTIATION
(2017)
Correction
Biochemistry & Molecular Biology
Si Xie, Oliver Mortusewicz, Hoi Tang Ma, Patrick Herr, Randy Y. C. Poon, Thomas Helleday, Chengmin Qian
Article
Oncology
Shan Huang, Rui Tang, Randy Y. C. Poon
Article
Cell Biology
Hoi Tang Ma, Randy Yat Choi Poon
Article
Neurosciences
Fei Ye, Eunchai Kang, Chuan Yu, Xuyu Qian, Fadi Jacob, Cong Yu, Mao Mao, Randy Y. C. Poon, Jieun Kim, Hongjun Song, Guo-li Ming, Mingjie Zhang
Article
Oncology
Wei Xia, Chung Mau Lo, Randy Y. C. Poon, Tan To Cheung, Albert C. Y. Chan, Lin Chen, Sitian Yang, George S. W. Tsao, Xiao Qi Wang
Article
Cell Biology
Hoi Tang Ma, Randy Y. C. Poon
Article
Cell Biology
Lau Yan Ng, Hoi Tang Ma, Julio C. Y. Liu, Xiner Huang, Nelson Lee, Randy Y. C. Poon
Article
Cell Biology
Xiaofang Zeng, Wendy Kaichun Xu, Tsun Ming Lok, Hoi Tang Ma, Randy Y. C. Poon
CELL DEATH & DISEASE
(2019)
Article
Oncology
Joyce P. Y. Mak, Hoi Tang Ma, Randy Y. C. Poon
MOLECULAR CANCER THERAPEUTICS
(2020)
Article
Biochemistry & Molecular Biology
Yang Wang, Randy Y. C. Poon
Summary: The anti-apoptotic protein MCL1 is gradually degraded during mitotic arrest, and the initial level of MCL1 is determined by the mitochondrial ubiquitin ligase MARCH5. Knockout of MARCH5 increases mitotic MCL1 but paradoxically enhances mitotic apoptosis in a BAK-dependent manner. MARCH5 regulates mitotic apoptosis through mechanisms independent of MCL1, including mitochondrial maintenance.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemistry & Molecular Biology
Lau Yan Ng, Hoi Tang Ma, Randy Y. C. Poon
Summary: In this study, an inverse relationship between cyclin A and CDC25A, two CDK activators, was uncovered. Destruction of cyclin A promoted an acute accumulation of CDC25A, mainly through transcriptional upregulation rather than protein stability changes. This compensatory mechanism involving CDC25A ensures timely mitotic entry at different levels of cyclin A deficiency.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Tsz Yin Lau, Randy Y. C. Poon
Summary: Whole-genome duplication (WGD) is a common genomic abnormality in cancers that provides redundant genes and facilitates clonal evolution in cancer cells. After WGD, the increased DNA and centrosome burden lead to genome instability, caused by factors such as DNA damage, replication stress, and altered spindle morphology. Cancer cells can overcome these obstacles through mechanisms like attenuating the p53-dependent G(1) checkpoint and forming pseudobipolar spindles with supernumerary centrosomes. These survival tactics and resulting genome instability confer proliferative advantage and therapeutic resistance to polyploid cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Tsz Kwan Yeung, Sehong Kim, Hoi Tang Ma, Randy Y. C. Poon
Summary: Switching genes on and off is crucial for understanding their functions. A contemporary approach involves using CRISPR to knockout genes and then turning off the rescue construct to study gene inactivation. This study developed a pair of switches controlled by inducible promoters and degrons, allowing efficient and tight regulation of gene activation and inactivation.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemical Research Methods
Randy Y. C. Poon
CELL CYCLE OSCILLATORS: METHODS AND PROTOCOLS
(2016)